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The map label for this gene is thiG

Identifier: 209696381

GI number: 209696381

Start: 3234523

End: 3235293

Strand: Direct

Name: thiG

Synonym: VSAL_I2987

Alternate gene names: 209696381

Gene position: 3234523-3235293 (Clockwise)

Preceding gene: 209696380

Following gene: 209696382

Centisome position: 97.27

GC content: 46.3

Gene sequence:

>771_bases
ATGACACAACCATTGATTATCGCAAATACACCATTTTCTTCTCGTTTGTTTACTGGAACAGGAAAATTCCCCAATTCGTT
AATAATGCAGCAGGCATTAATTGAATCGGGTTCTGAGCTTGCAACGATGGCGTTGAAACGAGTTGATGTGAGCAATCCTG
AGGATGACATTTTAAAGCCAATTATGACGGCGGGCATAAATTTACTGCCTAATACGTCAGGCGCTAAAAATGCCCGAGAA
GCGATCTTTGCGGCTCAGTTAGCCAGAGAGGCGTTGCAAACTAATTGGTTGAAATTGGAAATCCACCCTGATCCAAAATA
CCTAATGCCCGATCCGATTGAGACATTAAAAGCGGCGGAAGAATTAGTTCGCCAAGGATTTATTGTCTTGCCTTATTGTC
ACGCTGATCCGGTGTTATGCAAACGACTTGAAGAAGTCGGTTGTGCGGCAGTGATGCCATTGGGGGCGCCTATTGGGTCA
AACAAAGGCATTGTGAGTCGTGATTTTTTAGAGATTATTATCGATCAAGCAAAAGTGCCCGTTGTGGTTGATGCTGGAAT
TGGCGCACCATCGCATGCGGCCTTTGCGATGGAGTTGGGTGCTGATGCGGTATTGGTGAACACCGCGATTGCGGCAGCAA
GAAATCCAGTGGCGATGGCTACGGCATTTAAGTTGGCGGTTCAGTCTGGACGGTTAGCGTATGAAAATGGGTTGGTGTCT
GTGAGTTCTCAAGCGGTGGCATCGAGCCCATTAACGGCATTTTTGGATTAA

Upstream 100 bases:

>100_bases
GCTATTGACCAACAAATCGTTCCTCGAAGTGAATGGCAACAACGTGGTTTATCTGACGGTGAATCGATTTCTTTATTCCA
AGCCATTGCAGGAGGCTGAT

Downstream 100 bases:

>100_bases
CTCCCAAGGCTAAGGAAAGACGATGACGTTTACTGATGAATGGAAAAAACTGAATTGGGATGAGGTCAAACTCTCCATCT
ACAGTAAAACAGCGCAAGAC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTQPLIIANTPFSSRLFTGTGKFPNSLIMQQALIESGSELATMALKRVDVSNPEDDILKPIMTAGINLLPNTSGAKNARE
AIFAAQLAREALQTNWLKLEIHPDPKYLMPDPIETLKAAEELVRQGFIVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGS
NKGIVSRDFLEIIIDQAKVPVVVDAGIGAPSHAAFAMELGADAVLVNTAIAAARNPVAMATAFKLAVQSGRLAYENGLVS
VSSQAVASSPLTAFLD

Sequences:

>Translated_256_residues
MTQPLIIANTPFSSRLFTGTGKFPNSLIMQQALIESGSELATMALKRVDVSNPEDDILKPIMTAGINLLPNTSGAKNARE
AIFAAQLAREALQTNWLKLEIHPDPKYLMPDPIETLKAAEELVRQGFIVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGS
NKGIVSRDFLEIIIDQAKVPVVVDAGIGAPSHAAFAMELGADAVLVNTAIAAARNPVAMATAFKLAVQSGRLAYENGLVS
VSSQAVASSPLTAFLD
>Mature_255_residues
TQPLIIANTPFSSRLFTGTGKFPNSLIMQQALIESGSELATMALKRVDVSNPEDDILKPIMTAGINLLPNTSGAKNAREA
IFAAQLAREALQTNWLKLEIHPDPKYLMPDPIETLKAAEELVRQGFIVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSN
KGIVSRDFLEIIIDQAKVPVVVDAGIGAPSHAAFAMELGADAVLVNTAIAAARNPVAMATAFKLAVQSGRLAYENGLVSV
SSQAVASSPLTAFLD

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=69.8412698412698, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ALISL (B6EGV5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264312.1
- ProteinModelPortal:   B6EGV5
- GeneID:   6989291
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2987
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27087; Mature: 26956

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: ACT_SITE 98-98 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPLIIANTPFSSRLFTGTGKFPNSLIMQQALIESGSELATMALKRVDVSNPEDDILKP
CCCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHH
IMTAGINLLPNTSGAKNAREAIFAAQLAREALQTNWLKLEIHPDPKYLMPDPIETLKAAE
HHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
ELVRQGFIVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGIVSRDFLEIIIDQAKVP
HHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCC
VVVDAGIGAPSHAAFAMELGADAVLVNTAIAAARNPVAMATAFKLAVQSGRLAYENGLVS
EEEECCCCCCCCHHEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEE
VSSQAVASSPLTAFLD
ECCHHHHCCCCHHHCC
>Mature Secondary Structure 
TQPLIIANTPFSSRLFTGTGKFPNSLIMQQALIESGSELATMALKRVDVSNPEDDILKP
CCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHH
IMTAGINLLPNTSGAKNAREAIFAAQLAREALQTNWLKLEIHPDPKYLMPDPIETLKAAE
HHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
ELVRQGFIVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGIVSRDFLEIIIDQAKVP
HHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCC
VVVDAGIGAPSHAAFAMELGADAVLVNTAIAAARNPVAMATAFKLAVQSGRLAYENGLVS
EEEECCCCCCCCHHEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEE
VSSQAVASSPLTAFLD
ECCHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA