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The map label for this gene is thiH [H]

Identifier: 209696382

GI number: 209696382

Start: 3235316

End: 3236428

Strand: Direct

Name: thiH [H]

Synonym: VSAL_I2988

Alternate gene names: 209696382

Gene position: 3235316-3236428 (Clockwise)

Preceding gene: 209696381

Following gene: 209696386

Centisome position: 97.3

GC content: 42.5

Gene sequence:

>1113_bases
ATGACGTTTACTGATGAATGGAAAAAACTGAATTGGGATGAGGTCAAACTCTCCATCTACAGTAAAACAGCGCAAGACGT
AGAAAGAGCGCTGAACAAACCAAAGCGCAATTTAGACGATTTTAAAGCGTTAATCTCACCAGCCGCTGAAGGGTATTTAG
AGCAAATGGCTCAAGAGTCCGCACGACTGACTCGCCAGCGATTTGGTCATACCATGGGGTTTTATATTCCTCTTTATCTT
TCGAACCTATGTGCAAACGCGTGTACTTATTGTGGGTTCTCGATGGAGAATCGAATTAAACGAAAGACCTTGAATCAAGA
TGAAATTGCTAATGAGATTGATGCGATTAAAGCGATGAATTTTGACAGTGTACTTTTGGTGACTGGAGAGCATGAAACCA
AAGTTGGGATGAATTATTTCCGTGATACGGTTCCTCAAATTAAGCGGTCATTTAATTATTTGGCCATGGAAGTTCAACCA
TTAGATCAACACGAATACGCAGAACTAAAAACATTGGGTTTAGATGCCGTGATGGTGTACCAAGAAACGTACCATCCATC
GACGTATGCGGAACATCATTTGCGAGGTAACAAAACGGACTTTAACTACCGCTTAGAAACGCCGGACCGATTAGCCAAAG
CAGGGATTGATAAAATAGGCATTGGTGCCTTGATTGGTTTAGAAGAGTGGCGCACCGATTGTTTTTATGTGGCTGCGCAT
TTAGATTACTTGGAGCGTACGTACTGGCAAACAAGGTACTCGATTTCCTTTCCACGTTTGCGTCCATGTGAAGGCGCTTT
GCAACCCAAATCAATCATGAGTGATAAACAGCTTGTGCAGTTAATCTGTGCATACCGTTTGTTAAATTCGGAAGTTGAAT
TGTCACTGTCTACTCGTGAGTCTGAACATTTTAGAAATAATGTATTACCGCTTGGTATTACCTCTGTTTCTGCAGCATCA
AAAACTCAACCTGGAGGTTATGCAAATAACGAAGAGGCATTAGAGCAATTTGCTATTAGTGATGAGCGTTCAGCGTCTGC
GGTTGCACAAGCGGTAAAGGCGAGGGGATTTGAACCGGTGTGGAAAGATTGGGAGCGAAGTTATTCGGGTTAG

Upstream 100 bases:

>100_bases
TTAGCGTATGAAAATGGGTTGGTGTCTGTGAGTTCTCAAGCGGTGGCATCGAGCCCATTAACGGCATTTTTGGATTAACT
CCCAAGGCTAAGGAAAGACG

Downstream 100 bases:

>100_bases
TGAACTGTCGCGACGAACCGTGTTTCACGTGAAACTGTTTTCCTCTTCTTTGCTGTATTGAAATTAAAAAGCCGTCATTA
CCATTAAGGTGGTGACGGCT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTFTDEWKKLNWDEVKLSIYSKTAQDVERALNKPKRNLDDFKALISPAAEGYLEQMAQESARLTRQRFGHTMGFYIPLYL
SNLCANACTYCGFSMENRIKRKTLNQDEIANEIDAIKAMNFDSVLLVTGEHETKVGMNYFRDTVPQIKRSFNYLAMEVQP
LDQHEYAELKTLGLDAVMVYQETYHPSTYAEHHLRGNKTDFNYRLETPDRLAKAGIDKIGIGALIGLEEWRTDCFYVAAH
LDYLERTYWQTRYSISFPRLRPCEGALQPKSIMSDKQLVQLICAYRLLNSEVELSLSTRESEHFRNNVLPLGITSVSAAS
KTQPGGYANNEEALEQFAISDERSASAVAQAVKARGFEPVWKDWERSYSG

Sequences:

>Translated_370_residues
MTFTDEWKKLNWDEVKLSIYSKTAQDVERALNKPKRNLDDFKALISPAAEGYLEQMAQESARLTRQRFGHTMGFYIPLYL
SNLCANACTYCGFSMENRIKRKTLNQDEIANEIDAIKAMNFDSVLLVTGEHETKVGMNYFRDTVPQIKRSFNYLAMEVQP
LDQHEYAELKTLGLDAVMVYQETYHPSTYAEHHLRGNKTDFNYRLETPDRLAKAGIDKIGIGALIGLEEWRTDCFYVAAH
LDYLERTYWQTRYSISFPRLRPCEGALQPKSIMSDKQLVQLICAYRLLNSEVELSLSTRESEHFRNNVLPLGITSVSAAS
KTQPGGYANNEEALEQFAISDERSASAVAQAVKARGFEPVWKDWERSYSG
>Mature_369_residues
TFTDEWKKLNWDEVKLSIYSKTAQDVERALNKPKRNLDDFKALISPAAEGYLEQMAQESARLTRQRFGHTMGFYIPLYLS
NLCANACTYCGFSMENRIKRKTLNQDEIANEIDAIKAMNFDSVLLVTGEHETKVGMNYFRDTVPQIKRSFNYLAMEVQPL
DQHEYAELKTLGLDAVMVYQETYHPSTYAEHHLRGNKTDFNYRLETPDRLAKAGIDKIGIGALIGLEEWRTDCFYVAAHL
DYLERTYWQTRYSISFPRLRPCEGALQPKSIMSDKQLVQLICAYRLLNSEVELSLSTRESEHFRNNVLPLGITSVSAASK
TQPGGYANNEEALEQFAISDERSASAVAQAVKARGFEPVWKDWERSYSG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=370, Percent_Identity=64.3243243243243, Blast_Score=510, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42388; Mature: 42257

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTDEWKKLNWDEVKLSIYSKTAQDVERALNKPKRNLDDFKALISPAAEGYLEQMAQES
CCCCHHHHHCCHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARLTRQRFGHTMGFYIPLYLSNLCANACTYCGFSMENRIKRKTLNQDEIANEIDAIKAMN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
FDSVLLVTGEHETKVGMNYFRDTVPQIKRSFNYLAMEVQPLDQHEYAELKTLGLDAVMVY
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEE
QETYHPSTYAEHHLRGNKTDFNYRLETPDRLAKAGIDKIGIGALIGLEEWRTDCFYVAAH
HHHCCCCHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCEGALQPKSIMSDKQLVQLICAYRLLNSEVELSLSTRE
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCH
SEHFRNNVLPLGITSVSAASKTQPGGYANNEEALEQFAISDERSASAVAQAVKARGFEPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
WKDWERSYSG
HHHHHHCCCC
>Mature Secondary Structure 
TFTDEWKKLNWDEVKLSIYSKTAQDVERALNKPKRNLDDFKALISPAAEGYLEQMAQES
CCCHHHHHCCHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARLTRQRFGHTMGFYIPLYLSNLCANACTYCGFSMENRIKRKTLNQDEIANEIDAIKAMN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
FDSVLLVTGEHETKVGMNYFRDTVPQIKRSFNYLAMEVQPLDQHEYAELKTLGLDAVMVY
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEE
QETYHPSTYAEHHLRGNKTDFNYRLETPDRLAKAGIDKIGIGALIGLEEWRTDCFYVAAH
HHHCCCCHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCEGALQPKSIMSDKQLVQLICAYRLLNSEVELSLSTRE
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCH
SEHFRNNVLPLGITSVSAASKTQPGGYANNEEALEQFAISDERSASAVAQAVKARGFEPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
WKDWERSYSG
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]