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The map label for this gene is thiF [H]

Identifier: 209696379

GI number: 209696379

Start: 3233468

End: 3234316

Strand: Direct

Name: thiF [H]

Synonym: VSAL_I2985

Alternate gene names: 209696379

Gene position: 3233468-3234316 (Clockwise)

Preceding gene: 209696378

Following gene: 209696380

Centisome position: 97.24

GC content: 42.17

Gene sequence:

>849_bases
ATGAGTAATTTATTGATGGAAGGATTAAATGATCAAGATTTCCTTCGTTATAGCCGACAAATCATGTTGCCTGATATTGG
TGAGCGAGGACAATTGTCTCTTCGAAATAGCACCGTGCTAATGGTTGGCTGCGGAGGATTGGGATCATCTGTTGGAATGT
ATTTGGCTGCCGCTGGCGTGGGAACGTTGATTATTGCCGATGGCGATGACGTTGATCTTAGTAACTTACAGAGGCAAGTG
GTTTATCGTGAGCAGAGTATAAAACAGAATAAAGCGTTAGCGATGGCTCAAGAACTGCAATCAATCAACAAGACGATCAC
GATTGAGGTGATAACGAAGCATCTAACAGAACCTGCGCTTTCTCGATTCATTACTCAGGTAGATGTTGTTCTGGATTGCA
CTGATAACTTAACAACACGCCACAATATTAATGCCGCCTGTGTTCAACATCAGGTGCCTTTAATTTCTGGCGCGGCGATT
GGTTGGCAAGGGCAATTGATGTTGTTTTCAAATAAGAGTGATGAGTTAGGTAACACATCGTCGTCTTGTTATCACTGTCT
GTTCCCTTTTATTGAAAGCCAACAAACGAAGAACTGTCAATCTTTCGGAATTGTTGGGCCAGTGGTTGGCATGATAGGTA
ATCTACAAGCCTTAGAGACAATTAAGTATCTAACGCAACCTGAGTGGATGACATGGAGTTCACTGCATCAATTTAATGGT
AAGTCATTATCTTGGTCGACGATGGGTATTCCCAAAGACCATTGTTGTCCTGTCTGTCAGTCACCAAGTGATTTGCAAGA
TGTTTCACGTGAAACACATCATAAAGAGAGAGAATCGGTCAGTGAATAA

Upstream 100 bases:

>100_bases
GACAATATAGAAAAAGCCGTCAATCAGTTTTCTGCGATTTTAGCCCAACCAAGAACATCCGATTACCTTCTTAAATCGCA
AAATACACTGGAGGTGATGG

Downstream 100 bases:

>100_bases
TTTAATTAGTGTGGAATTAAATGGGGTTGTGCATCGGGTTGAAAAGAAAGTGACGTTATTCATGTTTCTATCTCATCAAC
AAATAGAAACTCAAGGGTGT

Product: thiamine biosynthesis adenylyltransferase ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSNLLMEGLNDQDFLRYSRQIMLPDIGERGQLSLRNSTVLMVGCGGLGSSVGMYLAAAGVGTLIIADGDDVDLSNLQRQV
VYREQSIKQNKALAMAQELQSINKTITIEVITKHLTEPALSRFITQVDVVLDCTDNLTTRHNINAACVQHQVPLISGAAI
GWQGQLMLFSNKSDELGNTSSSCYHCLFPFIESQQTKNCQSFGIVGPVVGMIGNLQALETIKYLTQPEWMTWSSLHQFNG
KSLSWSTMGIPKDHCCPVCQSPSDLQDVSRETHHKERESVSE

Sequences:

>Translated_282_residues
MSNLLMEGLNDQDFLRYSRQIMLPDIGERGQLSLRNSTVLMVGCGGLGSSVGMYLAAAGVGTLIIADGDDVDLSNLQRQV
VYREQSIKQNKALAMAQELQSINKTITIEVITKHLTEPALSRFITQVDVVLDCTDNLTTRHNINAACVQHQVPLISGAAI
GWQGQLMLFSNKSDELGNTSSSCYHCLFPFIESQQTKNCQSFGIVGPVVGMIGNLQALETIKYLTQPEWMTWSSLHQFNG
KSLSWSTMGIPKDHCCPVCQSPSDLQDVSRETHHKERESVSE
>Mature_281_residues
SNLLMEGLNDQDFLRYSRQIMLPDIGERGQLSLRNSTVLMVGCGGLGSSVGMYLAAAGVGTLIIADGDDVDLSNLQRQVV
YREQSIKQNKALAMAQELQSINKTITIEVITKHLTEPALSRFITQVDVVLDCTDNLTTRHNINAACVQHQVPLISGAAIG
WQGQLMLFSNKSDELGNTSSSCYHCLFPFIESQQTKNCQSFGIVGPVVGMIGNLQALETIKYLTQPEWMTWSSLHQFNGK
SLSWSTMGIPKDHCCPVCQSPSDLQDVSRETHHKERESVSE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=216, Percent_Identity=41.2037037037037, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI150417996, Length=153, Percent_Identity=28.7581699346405, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI225543285, Length=166, Percent_Identity=28.9156626506024, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI225543288, Length=166, Percent_Identity=28.9156626506024, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4885585, Length=159, Percent_Identity=28.9308176100629, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4885649, Length=149, Percent_Identity=26.8456375838926, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082356, Length=251, Percent_Identity=45.8167330677291, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1787048, Length=254, Percent_Identity=37.7952755905512, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1789177, Length=138, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=256, Percent_Identity=38.671875, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI193203301, Length=152, Percent_Identity=27.6315789473684, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=239, Percent_Identity=30.5439330543933, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320598, Length=143, Percent_Identity=32.1678321678322, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322639, Length=160, Percent_Identity=26.25, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582879, Length=253, Percent_Identity=38.3399209486166, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24660640, Length=154, Percent_Identity=30.5194805194805, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573937, Length=161, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 31094; Mature: 30963

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLMEGLNDQDFLRYSRQIMLPDIGERGQLSLRNSTVLMVGCGGLGSSVGMYLAAAGV
CCCCHHCCCCHHHHHHHHHHEECCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHCCC
GTLIIADGDDVDLSNLQRQVVYREQSIKQNKALAMAQELQSINKTITIEVITKHLTEPAL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHCHHHH
SRFITQVDVVLDCTDNLTTRHNINAACVQHQVPLISGAAIGWQGQLMLFSNKSDELGNTS
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHCCCEEECCEECCCCEEEEEECCCCCCCCCC
SSCYHCLFPFIESQQTKNCQSFGIVGPVVGMIGNLQALETIKYLTQPEWMTWSSLHQFNG
CHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCC
KSLSWSTMGIPKDHCCPVCQSPSDLQDVSRETHHKERESVSE
CCEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNLLMEGLNDQDFLRYSRQIMLPDIGERGQLSLRNSTVLMVGCGGLGSSVGMYLAAAGV
CCCHHCCCCHHHHHHHHHHEECCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHCCC
GTLIIADGDDVDLSNLQRQVVYREQSIKQNKALAMAQELQSINKTITIEVITKHLTEPAL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHCHHHH
SRFITQVDVVLDCTDNLTTRHNINAACVQHQVPLISGAAIGWQGQLMLFSNKSDELGNTS
HHHHHHHHHEEECCCCCCCCCCCCHHHHHHCCCEEECCEECCCCEEEEEECCCCCCCCCC
SSCYHCLFPFIESQQTKNCQSFGIVGPVVGMIGNLQALETIKYLTQPEWMTWSSLHQFNG
CHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCC
KSLSWSTMGIPKDHCCPVCQSPSDLQDVSRETHHKERESVSE
CCEEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]