Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 209696378

GI number: 209696378

Start: 3232795

End: 3233475

Strand: Direct

Name: thiE [H]

Synonym: VSAL_I2984

Alternate gene names: 209696378

Gene position: 3232795-3233475 (Clockwise)

Preceding gene: 209696377

Following gene: 209696379

Centisome position: 97.22

GC content: 40.68

Gene sequence:

>681_bases
ATGTATTCTTTTCCTGCAATCGATAGAGAAAAGTTTGGCCTTTACCCGGTGGTAGATGATGTGTCATGGATAGAGACATT
ACTTAAACTTAATGTTCAAACCATTCAATTACGAATTAAAAACTCGGAACACCCTGATTTAGAAAAGCAGATTATTGAAG
CGATTAGACTCGGTCGTGAATATGATGCTCAAGTTTTTATTAATGATTATTGGCAACTAGCAATAAAGCATGGCGCGTTT
GGTATTCATCTTGGACAAGAAGACATTGAAGTGGCCGATTTACTTGCCATCTCTGATGCCGGTATTTGTTTAGGATTATC
TACTCACGATGACAGTGAGTTAATGACCGTTAAAGCATTGAATCCAAGTTACTTAGCTCTTGGTCATGTTTTCCCTACGC
CGACCAAAGACATGCCATCAGAGCCTCAAGGTTTAGTTAACTTAGCGAACAACAGAAAATTAGCGGGTGAAACACCAACC
GTTGCTATCGGTGGTATTGATTTAACGGTGGCTGAGCAAGTGTGGCAAACCGGAGTTGATAGCATTGCGGTCGTTCGAGC
TATCACTCAAGCCGACAATATAGAAAAAGCCGTCAATCAGTTTTCTGCGATTTTAGCCCAACCAAGAACATCCGATTACC
TTCTTAAATCGCAAAATACACTGGAGGTGATGGATGAGTAA

Upstream 100 bases:

>100_bases
TGTAGATGCAGAACCCGTGAAAAGCTAACGACAGTTACCATTATTATTTTAATCATGAATGGTGGTGTTTCACGTGGAAC
ACCACTCTGGAGATCAAACG

Downstream 100 bases:

>100_bases
TTTATTGATGGAAGGATTAAATGATCAAGATTTCCTTCGTTATAGCCGACAAATCATGTTGCCTGATATTGGTGAGCGAG
GACAATTGTCTCTTCGAAAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MYSFPAIDREKFGLYPVVDDVSWIETLLKLNVQTIQLRIKNSEHPDLEKQIIEAIRLGREYDAQVFINDYWQLAIKHGAF
GIHLGQEDIEVADLLAISDAGICLGLSTHDDSELMTVKALNPSYLALGHVFPTPTKDMPSEPQGLVNLANNRKLAGETPT
VAIGGIDLTVAEQVWQTGVDSIAVVRAITQADNIEKAVNQFSAILAQPRTSDYLLKSQNTLEVMDE

Sequences:

>Translated_226_residues
MYSFPAIDREKFGLYPVVDDVSWIETLLKLNVQTIQLRIKNSEHPDLEKQIIEAIRLGREYDAQVFINDYWQLAIKHGAF
GIHLGQEDIEVADLLAISDAGICLGLSTHDDSELMTVKALNPSYLALGHVFPTPTKDMPSEPQGLVNLANNRKLAGETPT
VAIGGIDLTVAEQVWQTGVDSIAVVRAITQADNIEKAVNQFSAILAQPRTSDYLLKSQNTLEVMDE
>Mature_226_residues
MYSFPAIDREKFGLYPVVDDVSWIETLLKLNVQTIQLRIKNSEHPDLEKQIIEAIRLGREYDAQVFINDYWQLAIKHGAF
GIHLGQEDIEVADLLAISDAGICLGLSTHDDSELMTVKALNPSYLALGHVFPTPTKDMPSEPQGLVNLANNRKLAGETPT
VAIGGIDLTVAEQVWQTGVDSIAVVRAITQADNIEKAVNQFSAILAQPRTSDYLLKSQNTLEVMDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=191, Percent_Identity=57.5916230366492, Blast_Score=218, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24986; Mature: 24986

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSFPAIDREKFGLYPVVDDVSWIETLLKLNVQTIQLRIKNSEHPDLEKQIIEAIRLGRE
CCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
YDAQVFINDYWQLAIKHGAFGIHLGQEDIEVADLLAISDAGICLGLSTHDDSELMTVKAL
CCCEEEEEHHHEEHHHCCCEEEEECCCHHHHHHHEEECCCCEEEEECCCCCCCEEEEEEC
NPSYLALGHVFPTPTKDMPSEPQGLVNLANNRKLAGETPTVAIGGIDLTVAEQVWQTGVD
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEECCCCCEEEECCCCHHHHHHHHHCCCH
SIAVVRAITQADNIEKAVNQFSAILAQPRTSDYLLKSQNTLEVMDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCC
>Mature Secondary Structure
MYSFPAIDREKFGLYPVVDDVSWIETLLKLNVQTIQLRIKNSEHPDLEKQIIEAIRLGRE
CCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
YDAQVFINDYWQLAIKHGAFGIHLGQEDIEVADLLAISDAGICLGLSTHDDSELMTVKAL
CCCEEEEEHHHEEHHHCCCEEEEECCCHHHHHHHEEECCCCEEEEECCCCCCCEEEEEEC
NPSYLALGHVFPTPTKDMPSEPQGLVNLANNRKLAGETPTVAIGGIDLTVAEQVWQTGVD
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEECCCCCEEEECCCCHHHHHHHHHCCCH
SIAVVRAITQADNIEKAVNQFSAILAQPRTSDYLLKSQNTLEVMDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA