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The map label for this gene is epsM [H]

Identifier: 209696313

GI number: 209696313

Start: 3158785

End: 3159276

Strand: Reverse

Name: epsM [H]

Synonym: VSAL_I2915

Alternate gene names: 209696313

Gene position: 3159276-3158785 (Counterclockwise)

Preceding gene: 209696314

Following gene: 209696312

Centisome position: 95.01

GC content: 40.65

Gene sequence:

>492_bases
ATGAAAGCATTAATGACATGGTGGCAAAGTATTTCACCACGTGAACGTATTTTGGTTGGTGGTGGTAGCATCGCTTTGTT
GATCGCTTTGATTTATTGGGGAGGTATAAAACCCCTCAATGAAAGAGCGGAACTGGCTCAAAATCGTATTAACAGTGAAC
GTAATTTATTGGCTTGGGTACAAAAGAAAGCCGATAACATAACGACATTACGTGGTGGTACTTCTCATAATGCACAAACC
AGCATTAAACCGATGAATCAAGTCATACCAAGTAGTACCAATCGTTTTAAGATTGAACTGATCAGAATGCAACCTCGCGG
TGACGTTATGCAAGTGTGGGTGAAACCATTGCCATTTAATTCATTGATAAACTGGTTGGCTTATTTAAGAGACGCACAGG
GCATTGATGTGCAGTTTTTAGATATTGATAAAGCCGAAGTGGAAGGTATGGTTGAAGTGAATCGTCTGCAGCTAAGTCGA
GAGGCATCATGA

Upstream 100 bases:

>100_bases
AATTGACGTCAGAATTGGGCACTCAATTTGTGGTCGAACAAGGACAACTTAATAAAAACCAAGGCGAAGTTTTTGGTGCC
ATTGTCATTAGGAGAAAATA

Downstream 100 bases:

>100_bases
AGTTTAAATTATTGATTGCGACGGTATTTTCGACGTTTTTTACTTTTAGTGCGTTATTGCATATGCCGATTCAATGGGTG
ATTGACCAAGCACCAAAAGT

Product: general secretion pathway protein M

Products: NA

Alternate protein names: Cholera toxin secretion protein epsM [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKALMTWWQSISPRERILVGGGSIALLIALIYWGGIKPLNERAELAQNRINSERNLLAWVQKKADNITTLRGGTSHNAQT
SIKPMNQVIPSSTNRFKIELIRMQPRGDVMQVWVKPLPFNSLINWLAYLRDAQGIDVQFLDIDKAEVEGMVEVNRLQLSR
EAS

Sequences:

>Translated_163_residues
MKALMTWWQSISPRERILVGGGSIALLIALIYWGGIKPLNERAELAQNRINSERNLLAWVQKKADNITTLRGGTSHNAQT
SIKPMNQVIPSSTNRFKIELIRMQPRGDVMQVWVKPLPFNSLINWLAYLRDAQGIDVQFLDIDKAEVEGMVEVNRLQLSR
EAS
>Mature_163_residues
MKALMTWWQSISPRERILVGGGSIALLIALIYWGGIKPLNERAELAQNRINSERNLLAWVQKKADNITTLRGGTSHNAQT
SIKPMNQVIPSSTNRFKIELIRMQPRGDVMQVWVKPLPFNSLINWLAYLRDAQGIDVQFLDIDKAEVEGMVEVNRLQLSR
EAS

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3149

COG function: function code U; Type II secretory pathway, component PulM

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP M family [H]

Homologues:

Organism=Escherichia coli, GI1789339, Length=150, Percent_Identity=29.3333333333333, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007690
- InterPro:   IPR023229 [H]

Pfam domain/function: PF04612 GspM [H]

EC number: NA

Molecular weight: Translated: 18510; Mature: 18510

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALMTWWQSISPRERILVGGGSIALLIALIYWGGIKPLNERAELAQNRINSERNLLAWV
CCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
QKKADNITTLRGGTSHNAQTSIKPMNQVIPSSTNRFKIELIRMQPRGDVMQVWVKPLPFN
HHHCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCHH
SLINWLAYLRDAQGIDVQFLDIDKAEVEGMVEVNRLQLSREAS
HHHHHHHHHHCCCCCEEEEEECCHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKALMTWWQSISPRERILVGGGSIALLIALIYWGGIKPLNERAELAQNRINSERNLLAWV
CCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
QKKADNITTLRGGTSHNAQTSIKPMNQVIPSSTNRFKIELIRMQPRGDVMQVWVKPLPFN
HHHCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCHH
SLINWLAYLRDAQGIDVQFLDIDKAEVEGMVEVNRLQLSREAS
HHHHHHHHHHCCCCCEEEEEECCHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8406031; 10952301 [H]