Click here to switch to the map view.

The map label for this gene is epsL [H]

Identifier: 209696314

GI number: 209696314

Start: 3159276

End: 3160511

Strand: Reverse

Name: epsL [H]

Synonym: VSAL_I2916

Alternate gene names: 209696314

Gene position: 3160511-3159276 (Counterclockwise)

Preceding gene: 209696315

Following gene: 209696313

Centisome position: 95.05

GC content: 40.78

Gene sequence:

>1236_bases
CTGAATAGTCAACCATCAGATCCCATTCAATGGATCGTCTGGTCACCAGAACAAAAAGAAATCATCGCTTCGGGCGAATT
AGTTTCCTCTGAAACGTTATCCTCGTTAACTCAATACGCAGAACAACGCATTGTTTCTGTATTATTGCCGAGCAGTGATG
TTTTATTAAGAGAAGTCGCGATTCCAGAAGGGGCGGCTCGACAGTTTTCATCCATGTTACCTTTTATTATTGAAGATGAT
TTGGCTCAGGATGTAGATGATTTACATATCGTCATAATAAAGAAAAATAATAAAACAGCGCAAATTGCTATTGTTGAACA
TGAAAAAATGGACACTTGGATTACTCAGTTAAAGGACGCTGAAATTCACGCTAAACAGTGGTTACCAGATGTACTCGCTT
TGCCTTATCAAGAGGATGGCTTATCGCTCGTACAATTGAATGATCAATGGTTGATTCGTCATCAGGAGCATCAAGGTGCG
GTAGCTGAATCGAGTTGGGTTTCTGTATTGTTAGATGGCATGGTCATCTTCGCGAATGCACAGAAGTTAGAAAGCCAGAG
TGACGTCGATGAAGCGGAAAGTGAATCAACGCCTGTTGTTGTGATCCATGCTTATTCTCCTTGTGAAATAGAGATCGATA
ATGCCGAAGTACATATAGAATCCCCTGAATTGGTGATGCAGCTATTAGCTGAAGGTGCTATCCATTCCAAAGTGAATATA
TTAACGGGAAGGTATCGTCCTCAATCGTCTTGGCGCAAACACTGGCGGATATGGCAAAAAGTGATTCTGGCGTTTGGTTT
AGTGATGGTTGCGTTTGTTGGACAGCATATTTCTGAAGTGCAAAAATTAGAACAACACAGTGTTGCTCTTCGAGCTGAAA
GTGAACGTATTTTTCGTGAAATTTTTCCAGCGAAACGAAAAATACCAACCTCAAGTTATCTGAAGTCTCAAATTAAAAAT
GAAGAGATGCGCTTGCAAGGTGGAGCGGGAGGGAACGATCTTTTAGGCTGGATGGCCGAGCTTCAACCTTATTTAGCAAA
GGTGCCTCAAGTTAAACTAAAAAGTTTAAAATTTGATGGAAAGCGTGATGAGTTACGCCTTCAAGCCAGTGCTGCTGATT
TTCCTTATTTTGAGCAATTGACGTCAGAATTGGGCACTCAATTTGTGGTCGAACAAGGACAACTTAATAAAAACCAAGGC
GAAGTTTTTGGTGCCATTGTCATTAGGAGAAAATAA

Upstream 100 bases:

>100_bases
GTACGTGTGCGTTCTTTATTGCACAGCAGTGATAAGAAAGTGGTGAACGTTATCCGTCGCCAATATGGAGGAATGAGTGA
GCGAACATCTGATAATAAGG

Downstream 100 bases:

>100_bases
TGAAAGCATTAATGACATGGTGGCAAAGTATTTCACCACGTGAACGTATTTTGGTTGGTGGTGGTAGCATCGCTTTGTTG
ATCGCTTTGATTTATTGGGG

Product: general secretion pathway protein L

Products: NA

Alternate protein names: Cholera toxin secretion protein epsL [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNSQPSDPIQWIVWSPEQKEIIASGELVSSETLSSLTQYAEQRIVSVLLPSSDVLLREVAIPEGAARQFSSMLPFIIEDD
LAQDVDDLHIVIIKKNNKTAQIAIVEHEKMDTWITQLKDAEIHAKQWLPDVLALPYQEDGLSLVQLNDQWLIRHQEHQGA
VAESSWVSVLLDGMVIFANAQKLESQSDVDEAESESTPVVVIHAYSPCEIEIDNAEVHIESPELVMQLLAEGAIHSKVNI
LTGRYRPQSSWRKHWRIWQKVILAFGLVMVAFVGQHISEVQKLEQHSVALRAESERIFREIFPAKRKIPTSSYLKSQIKN
EEMRLQGGAGGNDLLGWMAELQPYLAKVPQVKLKSLKFDGKRDELRLQASAADFPYFEQLTSELGTQFVVEQGQLNKNQG
EVFGAIVIRRK

Sequences:

>Translated_411_residues
MNSQPSDPIQWIVWSPEQKEIIASGELVSSETLSSLTQYAEQRIVSVLLPSSDVLLREVAIPEGAARQFSSMLPFIIEDD
LAQDVDDLHIVIIKKNNKTAQIAIVEHEKMDTWITQLKDAEIHAKQWLPDVLALPYQEDGLSLVQLNDQWLIRHQEHQGA
VAESSWVSVLLDGMVIFANAQKLESQSDVDEAESESTPVVVIHAYSPCEIEIDNAEVHIESPELVMQLLAEGAIHSKVNI
LTGRYRPQSSWRKHWRIWQKVILAFGLVMVAFVGQHISEVQKLEQHSVALRAESERIFREIFPAKRKIPTSSYLKSQIKN
EEMRLQGGAGGNDLLGWMAELQPYLAKVPQVKLKSLKFDGKRDELRLQASAADFPYFEQLTSELGTQFVVEQGQLNKNQG
EVFGAIVIRRK
>Mature_411_residues
MNSQPSDPIQWIVWSPEQKEIIASGELVSSETLSSLTQYAEQRIVSVLLPSSDVLLREVAIPEGAARQFSSMLPFIIEDD
LAQDVDDLHIVIIKKNNKTAQIAIVEHEKMDTWITQLKDAEIHAKQWLPDVLALPYQEDGLSLVQLNDQWLIRHQEHQGA
VAESSWVSVLLDGMVIFANAQKLESQSDVDEAESESTPVVVIHAYSPCEIEIDNAEVHIESPELVMQLLAEGAIHSKVNI
LTGRYRPQSSWRKHWRIWQKVILAFGLVMVAFVGQHISEVQKLEQHSVALRAESERIFREIFPAKRKIPTSSYLKSQIKN
EEMRLQGGAGGNDLLGWMAELQPYLAKVPQVKLKSLKFDGKRDELRLQASAADFPYFEQLTSELGTQFVVEQGQLNKNQG
EVFGAIVIRRK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3297

COG function: function code U; Type II secretory pathway, component PulL

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

Organism=Escherichia coli, GI87082244, Length=126, Percent_Identity=25.3968253968254, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007812 [H]

Pfam domain/function: PF05134 GspL [H]

EC number: NA

Molecular weight: Translated: 46429; Mature: 46429

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQPSDPIQWIVWSPEQKEIIASGELVSSETLSSLTQYAEQRIVSVLLPSSDVLLREVA
CCCCCCCCCEEEEECCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
IPEGAARQFSSMLPFIIEDDLAQDVDDLHIVIIKKNNKTAQIAIVEHEKMDTWITQLKDA
CCCHHHHHHHHHCCCEEHHHHHCCCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHCCC
EIHAKQWLPDVLALPYQEDGLSLVQLNDQWLIRHQEHQGAVAESSWVSVLLDGMVIFANA
HHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCEEEEECH
QKLESQSDVDEAESESTPVVVIHAYSPCEIEIDNAEVHIESPELVMQLLAEGAIHSKVNI
HHHCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCEEEECCHHHHHHHHHCCCHHCEEEE
LTGRYRPQSSWRKHWRIWQKVILAFGLVMVAFVGQHISEVQKLEQHSVALRAESERIFRE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFPAKRKIPTSSYLKSQIKNEEMRLQGGAGGNDLLGWMAELQPYLAKVPQVKLKSLKFDG
HCCHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
KRDELRLQASAADFPYFEQLTSELGTQFVVEQGQLNKNQGEVFGAIVIRRK
CCCCEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNSQPSDPIQWIVWSPEQKEIIASGELVSSETLSSLTQYAEQRIVSVLLPSSDVLLREVA
CCCCCCCCCEEEEECCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
IPEGAARQFSSMLPFIIEDDLAQDVDDLHIVIIKKNNKTAQIAIVEHEKMDTWITQLKDA
CCCHHHHHHHHHCCCEEHHHHHCCCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHCCC
EIHAKQWLPDVLALPYQEDGLSLVQLNDQWLIRHQEHQGAVAESSWVSVLLDGMVIFANA
HHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCEEEEECH
QKLESQSDVDEAESESTPVVVIHAYSPCEIEIDNAEVHIESPELVMQLLAEGAIHSKVNI
HHHCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCEEEECCHHHHHHHHHCCCHHCEEEE
LTGRYRPQSSWRKHWRIWQKVILAFGLVMVAFVGQHISEVQKLEQHSVALRAESERIFRE
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFPAKRKIPTSSYLKSQIKNEEMRLQGGAGGNDLLGWMAELQPYLAKVPQVKLKSLKFDG
HCCHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
KRDELRLQASAADFPYFEQLTSELGTQFVVEQGQLNKNQGEVFGAIVIRRK
CCCCEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]