Click here to switch to the map view.

The map label for this gene is nudE [H]

Identifier: 209696311

GI number: 209696311

Start: 3157466

End: 3158017

Strand: Reverse

Name: nudE [H]

Synonym: VSAL_I2913

Alternate gene names: 209696311

Gene position: 3158017-3157466 (Counterclockwise)

Preceding gene: 209696312

Following gene: 209696310

Centisome position: 94.97

GC content: 41.12

Gene sequence:

>552_bases
ATGGCTAAAAAACAATTGCCGACCATCTTAGATAAACGAATTGAAGCGAAATCAAAACTGTTTTGTATTGAATCGGTTGA
TCTGCGTTTTTCAAATGGTGTGGAACGTACTTATGAACGAATGAAACCAAGTGGTCGTCATGCGGTTATGATGGTTCCTG
TTACTGAGCAGGGTGATTTACTCTTAGTTCGTGAATATGCGGTTGGGACTGAACGCTATGAGCTTGGGTTCCCTAAAGGA
TTAATCGATGAAGGTGAAATGCCAATAGAAGCGGCAAACCGAGAATTGAAAGAAGAGATTGGCTTTGGTGCAGAGAAATT
AACAGCATTAAAAGAATTGGTTTTAGCGCCGTCTTATTTCTCGAGTAAAATGACGCTTTTTGTTGCCCAAGGCTTGTACC
CTGAGAGATTGGAAGGGGACGAACCTGAGCCATTGGACATTGTTCGCTGGCCATTAAGTCAAGCGTCTGAATTACTTCAT
CATCTCGATTTTTCAGAAGCAAGGTGCATTAGTGCATTATTGCTTGCTCAACAATTTTTAGAAAAGGAGTGA

Upstream 100 bases:

>100_bases
CTTCCCATCTAGCTTTAGAAGCCAGCTAAAATGGCTAGGCAAGCCTGATGCAAAAGGGCAATATAGAGTGACTTATTCCG
GACGTCTTTAAGGGTATATG

Downstream 100 bases:

>100_bases
TATTGATGGATTCTACGTTTTCACATCTTCTTCCTGACGTGATCAAAATTGCTCGCGCTTCAGGTCAATTGATTTTAGAT
ATCTATGAAAAAGGCGATTT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MAKKQLPTILDKRIEAKSKLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTERYELGFPKG
LIDEGEMPIEAANRELKEEIGFGAEKLTALKELVLAPSYFSSKMTLFVAQGLYPERLEGDEPEPLDIVRWPLSQASELLH
HLDFSEARCISALLLAQQFLEKE

Sequences:

>Translated_183_residues
MAKKQLPTILDKRIEAKSKLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTERYELGFPKG
LIDEGEMPIEAANRELKEEIGFGAEKLTALKELVLAPSYFSSKMTLFVAQGLYPERLEGDEPEPLDIVRWPLSQASELLH
HLDFSEARCISALLLAQQFLEKE
>Mature_182_residues
AKKQLPTILDKRIEAKSKLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTERYELGFPKGL
IDEGEMPIEAANRELKEEIGFGAEKLTALKELVLAPSYFSSKMTLFVAQGLYPERLEGDEPEPLDIVRWPLSQASELLHH
LDFSEARCISALLLAQQFLEKE

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=179, Percent_Identity=63.6871508379888, Blast_Score=230, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 20757; Mature: 20626

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQLPTILDKRIEAKSKLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDL
CCCCHHHHHHHHHHHHHHHEEEEEECCCHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCE
LLVREYAVGTERYELGFPKGLIDEGEMPIEAANRELKEEIGFGAEKLTALKELVLAPSYF
EEEEECCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
SSKMTLFVAQGLYPERLEGDEPEPLDIVRWPLSQASELLHHLDFSEARCISALLLAQQFL
HHHHEEEEECCCCHHHCCCCCCCCHHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EKE
HCC
>Mature Secondary Structure 
AKKQLPTILDKRIEAKSKLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDL
CCCHHHHHHHHHHHHHHHEEEEEECCCHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCE
LLVREYAVGTERYELGFPKGLIDEGEMPIEAANRELKEEIGFGAEKLTALKELVLAPSYF
EEEEECCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
SSKMTLFVAQGLYPERLEGDEPEPLDIVRWPLSQASELLHHLDFSEARCISALLLAQQFL
HHHHEEEEECCCCHHHCCCCCCCCHHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EKE
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]