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The map label for this gene is murI

Identifier: 209696283

GI number: 209696283

Start: 3128806

End: 3129603

Strand: Reverse

Name: murI

Synonym: VSAL_I2884

Alternate gene names: 209696283

Gene position: 3129603-3128806 (Counterclockwise)

Preceding gene: 209696284

Following gene: 209696276

Centisome position: 94.12

GC content: 34.46

Gene sequence:

>798_bases
ATGCCAAAAAACATCTTAATTTTTGACTCAGGTATCGGTGGTTTATCCGTATTTAAAGATATTAGATATCAAATTCCTTT
GGCAAAGTATATTTATGCTTTTGATAATGCAGGCTTTCCTTATGGAGAGTTAACAGAAGATATCTTAATAGAAAGAACAT
CTTATATTATTCAAAAGCTTTGTCATCGACATAAAATAGATATCGTTGTTATTGCCTGTAATACAGCAAGTACAGTGGTG
TTACCTACATTAAGAACACTTCTCTCTATTCCAGTTGTTGGGGTTGTTCCTGCTATTAAACCAGCAGCGCTTGTTTCTCA
AAAAATAGGATTACTTGCTACTCCTGCAACTATTAAACGAAGTTATACGTTTGACTTAATTAAATCATTTGCATCAATTT
CAGATGTGAAGTTATTAGGGTCGACTCGATTAGTTGAAATGGCTGAAGAGAAAATGGTAGGCATACCTGTCAATTTAATT
GAACTTGATGAGATTCTAAAGTCTTGGAAAGGTCAGGTTGATTGTATTGTACTTGGTTGTACGCATTTTCCTTTTCTACG
TAAAGAAATCAAAGAAATTCTTGGCCGTAACGTATTACTGATAGATTCAGGAGAAGCAATAGCAAGAAGAGTGAAGCAAT
TATTAGGGAGTGTTGACGCTAATAAAGGAGATCATTTGTTTGGGGAAGTTTATTGTAGTGCCTCAACAAAAAATGAAGAG
GCACTAAATAAGACTTTCAAAGAATTAGATTTTAATCCTCTTCAGACTCTAGGCTACCCGAAGTCTTTGGATCGTTAG

Upstream 100 bases:

>100_bases
TACGCATCTACGTTATCAAGCTTCTATTTTACAACTGTAATCCAATCGGTATGATTGTATGTCTATCCCTTTTGTTATTT
GAAGAGCTTGTGGTTCCAAT

Downstream 100 bases:

>100_bases
CAATGCGAACTTTTAAAGTACGTTGATCAAATTCGTTATTGTTTAAGTTTTCAATAACATTATCAGCGTCCTTTGTTGCC
ACAACAACAAAACCAAATCC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPKNILIFDSGIGGLSVFKDIRYQIPLAKYIYAFDNAGFPYGELTEDILIERTSYIIQKLCHRHKIDIVVIACNTASTVV
LPTLRTLLSIPVVGVVPAIKPAALVSQKIGLLATPATIKRSYTFDLIKSFASISDVKLLGSTRLVEMAEEKMVGIPVNLI
ELDEILKSWKGQVDCIVLGCTHFPFLRKEIKEILGRNVLLIDSGEAIARRVKQLLGSVDANKGDHLFGEVYCSASTKNEE
ALNKTFKELDFNPLQTLGYPKSLDR

Sequences:

>Translated_265_residues
MPKNILIFDSGIGGLSVFKDIRYQIPLAKYIYAFDNAGFPYGELTEDILIERTSYIIQKLCHRHKIDIVVIACNTASTVV
LPTLRTLLSIPVVGVVPAIKPAALVSQKIGLLATPATIKRSYTFDLIKSFASISDVKLLGSTRLVEMAEEKMVGIPVNLI
ELDEILKSWKGQVDCIVLGCTHFPFLRKEIKEILGRNVLLIDSGEAIARRVKQLLGSVDANKGDHLFGEVYCSASTKNEE
ALNKTFKELDFNPLQTLGYPKSLDR
>Mature_264_residues
PKNILIFDSGIGGLSVFKDIRYQIPLAKYIYAFDNAGFPYGELTEDILIERTSYIIQKLCHRHKIDIVVIACNTASTVVL
PTLRTLLSIPVVGVVPAIKPAALVSQKIGLLATPATIKRSYTFDLIKSFASISDVKLLGSTRLVEMAEEKMVGIPVNLIE
LDEILKSWKGQVDCIVLGCTHFPFLRKEIKEILGRNVLLIDSGEAIARRVKQLLGSVDANKGDHLFGEVYCSASTKNEEA
LNKTFKELDFNPLQTLGYPKSLDR

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=262, Percent_Identity=46.1832061068702, Blast_Score=214, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_ALISL (B6ENT1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264214.1
- GeneID:   6987959
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2884
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29352; Mature: 29221

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNILIFDSGIGGLSVFKDIRYQIPLAKYIYAFDNAGFPYGELTEDILIERTSYIIQKL
CCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CHRHKIDIVVIACNTASTVVLPTLRTLLSIPVVGVVPAIKPAALVSQKIGLLATPATIKR
HHHCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCEECCHHHHH
SYTFDLIKSFASISDVKLLGSTRLVEMAEEKMVGIPVNLIELDEILKSWKGQVDCIVLGC
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEC
THFPFLRKEIKEILGRNVLLIDSGEAIARRVKQLLGSVDANKGDHLFGEVYCSASTKNEE
CCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHH
ALNKTFKELDFNPLQTLGYPKSLDR
HHHHHHHHCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
PKNILIFDSGIGGLSVFKDIRYQIPLAKYIYAFDNAGFPYGELTEDILIERTSYIIQKL
CCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CHRHKIDIVVIACNTASTVVLPTLRTLLSIPVVGVVPAIKPAALVSQKIGLLATPATIKR
HHHCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCEECCHHHHH
SYTFDLIKSFASISDVKLLGSTRLVEMAEEKMVGIPVNLIELDEILKSWKGQVDCIVLGC
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEEC
THFPFLRKEIKEILGRNVLLIDSGEAIARRVKQLLGSVDANKGDHLFGEVYCSASTKNEE
CCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHH
ALNKTFKELDFNPLQTLGYPKSLDR
HHHHHHHHCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA