Click here to switch to the map view.

The map label for this gene is purH

Identifier: 209696246

GI number: 209696246

Start: 3079982

End: 3081574

Strand: Direct

Name: purH

Synonym: VSAL_I2842

Alternate gene names: 209696246

Gene position: 3079982-3081574 (Clockwise)

Preceding gene: 209696243

Following gene: 209696247

Centisome position: 92.63

GC content: 45.7

Gene sequence:

>1593_bases
ATGAATAACCCACGTCCGATCCGTCGTGCGCTAATTAGCGTATCTGATAAAACTGGTATTGTAGAATTTGCTCAAGCACT
GACTGAACGTGGTGTAGATATTCTATCTACTGGCGGCACTGCTCGCCTGCTTGCGGAAAAAAATATCCCTGTAACAGAAG
TATCTGATTACACTGGCTTCCCAGAAATGATGGATGGCCGCGTTAAAACTCTTCATCCTAAAGTACATGGTGGTGTTCTT
GGTCGTCGCGGTCAAGATGATGGCATCATGGAGCAACACGGCATCAACCCAATCGATATTGTTGTTGTTAACCTTTATCC
ATTCGCTGAAACCGTAGCAAAAGAAGGCTGTACGCTAGCTGATGCTGTTGAGAACATCGATATCGGTGGTCCAACAATGG
TTCGCTCTGCGGCTAAAAACCACAAAGACGTTACTATTGTCGTTAATGCTCACGATTACGATCGCGTCATTGCTGAAATG
GACACTAACGACAAATCACTGACTCAAGCAACGCGTTTTGATTTAGCGATTGCAGCATTTGAGCACACCGCTTCTTACGA
TGGCATGATCGCTAATTACTTCGGCACTATGGTTCCTAGCTATGGTGAGAACAAAGAAGGCGATAACGAATCTAAATTCC
CTCGTACCTTCAACCAACAGTTCGAGAAAAAACAAGACATGCGCTACGGTGAGAACAGTCATCAAGCGGCTGCATTCTAT
GTTGAAGCTAATCCTGAAGAAGCGTCAGTATCAACGGCTCGTCAAATCCAAGGTAAAGCCCTTTCTTATAACAACATTGC
TGATACTGACGCCGCTCTTGAGTGTGTGAAAGAGTTCAACGAACCAGCATGTGTTATCGTTAAGCACGCAAACCCTTGTG
GTGTTGCATTAGGTAAAGACGTTCTTGAAGCTTATCACCGTGCTTACCAAACAGATCCTACCTCAGCATTTGGCGGCATC
ATTGCTTTCAACCGTGAATTAGATGCTGCGACTGCAACAGCGATTACTGAACGTCAATTTGTTGAAGTGATTATTGCCCC
TTCTGTTTCTGCTGAAGCAATTGAAATCATAGCGGCTAAGAAAAACCTTCGCTTACTTGAGTGTGGCGAATGGTCAACAA
AAACCACGGAATTTGATATTAAGCGTGTTAACGGCGGCCTATTGGTTCAAGACCGTGACCAAGGCATGGTATCGGAAGAT
GAGCTTGAAGTGGTTTCTAAGCGCCAACCAACGGCTGAAGAATTAAAAGACGCACTGTTCTGTTGGAAAGTAGCGAAATA
CGTGAAATCTAACGCCATTGTTTACTCTAAAGGTGACATGACTATCGGTGTCGGCGCTGGCCAAATGAGCCGCGTGTATT
CTGCAAAAATTGCAGGTATTAAAGCCGCGGACGAAGGTTTAGTGGTCGAAGGTTGCGCAATGGCATCCGATGCGTTCTTC
CCATTCCGTGATGGCATTGATGCAGCGGCAGAAGCTGGCATTAAATGTGTTATCCAACCGGGTGGTTCAATGCGTGATGA
TGAAGTTATCGCAGCAGCAGACGAGCATGGTATGGCGATGATCTTTACTGGTATGCGCCACTTCCGCCACTAA

Upstream 100 bases:

>100_bases
GGCCAATTACTGTTTGCCTTCTTTATTACTAAGAATGATAAACAGAACAAGTTCATTTTTGGCAAAAATCCTAACTTAAT
TCTAAAGGAAGAAAGTAGCA

Downstream 100 bases:

>100_bases
TTTATTCTTCATATTTAATACTGTAGCGTCGTTAACTACTTCCGCCCACCCCAGTCACATAGCACGCTATGCTCCCGGGA
TGGGCTCAATTGTTGCCTAG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNNPRPIRRALISVSDKTGIVEFAQALTERGVDILSTGGTARLLAEKNIPVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDGIMEQHGINPIDIVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DTNDKSLTQATRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDNESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDVLEAYHRAYQTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAIEIIAAKKNLRLLECGEWSTKTTEFDIKRVNGGLLVQDRDQGMVSED
ELEVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGCAMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Sequences:

>Translated_530_residues
MNNPRPIRRALISVSDKTGIVEFAQALTERGVDILSTGGTARLLAEKNIPVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDGIMEQHGINPIDIVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DTNDKSLTQATRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDNESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDVLEAYHRAYQTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAIEIIAAKKNLRLLECGEWSTKTTEFDIKRVNGGLLVQDRDQGMVSED
ELEVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGCAMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
>Mature_530_residues
MNNPRPIRRALISVSDKTGIVEFAQALTERGVDILSTGGTARLLAEKNIPVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDGIMEQHGINPIDIVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEM
DTNDKSLTQATRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDNESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDVLEAYHRAYQTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAIEIIAAKKNLRLLECGEWSTKTTEFDIKRVNGGLLVQDRDQGMVSED
ELEVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGCAMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=487, Percent_Identity=37.3716632443532, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=78.3427495291902, Blast_Score=867, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=511, Percent_Identity=32.8767123287671, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=371, Percent_Identity=25.3369272237197, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=35.3846153846154, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=618, Percent_Identity=33.0097087378641, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=36.2525458248472, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24649832, Length=613, Percent_Identity=34.910277324633, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_ALISL (B6ENA8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264176.1
- GeneID:   6987941
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2842
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57625; Mature: 57625

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPRPIRRALISVSDKTGIVEFAQALTERGVDILSTGGTARLLAEKNIPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDGIMEQHGINPIDIVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEEECHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEMDTNDKSLTQATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDNESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
VLEAYHRAYQTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSAEAIEIIAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCHHHEEEEEEC
KNLRLLECGEWSTKTTEFDIKRVNGGLLVQDRDQGMVSEDELEVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGCAMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEECCHHCCCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNNPRPIRRALISVSDKTGIVEFAQALTERGVDILSTGGTARLLAEKNIPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDGIMEQHGINPIDIVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEEECHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYDRVIAEMDTNDKSLTQATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDNESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
VLEAYHRAYQTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSAEAIEIIAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCHHHEEEEEEC
KNLRLLECGEWSTKTTEFDIKRVNGGLLVQDRDQGMVSEDELEVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGCAMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEECCHHCCCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA