Click here to switch to the map view.

The map label for this gene is fis

Identifier: 209696243

GI number: 209696243

Start: 3078449

End: 3078745

Strand: Direct

Name: fis

Synonym: VSAL_I2839

Alternate gene names: 209696243

Gene position: 3078449-3078745 (Clockwise)

Preceding gene: 209696242

Following gene: 209696246

Centisome position: 92.58

GC content: 39.39

Gene sequence:

>297_bases
ATGTTCGATCAATCTATGACTTCAGAAGCATTAACAGTTACAACAGTTACAGCACAAGACCAGATTACTCAGAAGCCACT
ACGTGACTCTGTTAAGGCTTCGCTGAAAAACTACTTAGGCCAATTAAACGGTCAAGAAGTTAACGACTTATATGAATTAG
TATTAGCTGAAGTAGAGCAACCTCTACTAGACATGATCATGCAGCATACTCGCGGCAACCAAACAAAAGCAGCAAATATG
ATGGGTATCAACCGTGGTACTCTTCGCAAGAAACTTAAAAAATACGGCATGAACTAA

Upstream 100 bases:

>100_bases
CGCGGCTTTAATGCTATTGAAGATGCCCATCAGCAACTTCAACAGCTTGAAAGCTATTTCGACCACGTTGCATCATAATA
ACAGAAGAGCTAAACAGAAT

Downstream 100 bases:

>100_bases
TCACGCCTTATGAAAAATTCAAAGCAGTATTAAGCAATTAACACTGCTTTTTTTATATCTAATAATCTTCATATTACTAG
ATATAAAAAAGCCGATACTA

Product: DNA-binding protein Fis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELVLAEVEQPLLDMIMQHTRGNQTKAANM
MGINRGTLRKKLKKYGMN

Sequences:

>Translated_98_residues
MFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELVLAEVEQPLLDMIMQHTRGNQTKAANM
MGINRGTLRKKLKKYGMN
>Mature_98_residues
MFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELVLAEVEQPLLDMIMQHTRGNQTKAANM
MGINRGTLRKKLKKYGMN

Specific function: Activates ribosomal RNA transcription. Plays a direct role in upstream activation of rRNA promoters

COG id: COG2901

COG function: function code KL; Factor for inversion stimulation Fis, transcriptional activator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transcriptional regulatory fis family

Homologues:

Organism=Escherichia coli, GI1789661, Length=98, Percent_Identity=80.6122448979592, Blast_Score=169, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIS_ALISL (B6ENA5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264173.1
- ProteinModelPortal:   B6ENA5
- SMR:   B6ENA5
- GeneID:   6988432
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2839
- HOGENOM:   HBG509520
- OMA:   EMVLCEV
- ProtClustDB:   PRK00430
- HAMAP:   MF_00166
- InterPro:   IPR005412
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- PIRSF:   PIRSF002097
- PRINTS:   PR01591
- PRINTS:   PR01590

Pfam domain/function: PF02954 HTH_8; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 11047; Mature: 11047

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELVLAEVEQ
CCCCHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
PLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKKYGMN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDQSMTSEALTVTTVTAQDQITQKPLRDSVKASLKNYLGQLNGQEVNDLYELVLAEVEQ
CCCCHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
PLLDMIMQHTRGNQTKAANMMGINRGTLRKKLKKYGMN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA