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The map label for this gene is rlmB [H]

Identifier: 209696173

GI number: 209696173

Start: 2995758

End: 2996501

Strand: Reverse

Name: rlmB [H]

Synonym: VSAL_I2767

Alternate gene names: 209696173

Gene position: 2996501-2995758 (Counterclockwise)

Preceding gene: 209696174

Following gene: 209696172

Centisome position: 90.12

GC content: 43.28

Gene sequence:

>744_bases
ATGAGTAATGAATTTCTTTTTGGTATCCACGCAACCAAAGCTGTATTAGAGCGTGATCCTGCCCGTTTCATTGAAATCTT
TGTATTAAAAGGTCGTGAAGACGATCGTTTATTACCGATCATCACTGAACTGGGTGAGCTTGGATTTAAGATCCAAGAAC
TTCCACGTAAAACGTTAGACGACAAAGCGAAAGGTGCAAATCACCAAGGTATTATTGCTCGTGTTACGCCTGCTAAGCAG
TTGAATGAACGTGACTTAGACGACATCATGGATAAAACAGAGAATCCATTATTCTTAGTTCTTGATGGTGTAACCGATCC
TCACAATCTTGGTGCTTGTTTGCGTAATGCTGATGGTGCAGGTGTGGCAGCTGTTATTGTTCCTAAAGACAAATCAGCAT
CAATGACAGCAACAGTAAGTAAAGTTGCTTGTGGTGCGGCTGAAATGGTGCCTCTTGTACGTGTAACTAACTTGGCTCGT
ACTATGCGTGCACTTCAAGAGAAAGGCGTATGGTTTGTTGGTACTGCTGGTGAAGCGACGAAAGATGTATTCCAATCGAA
GTTAACGGGTCCATTAGCTATTGTTATGGGTGCGGAAGGAGATGGCATGCGCCGTCTAACTCGCGAGACTTGTGATGACT
TGATTAAAATCCCTATGGCTGGCTCTGTAACCAGTTTAAACGTTTCTGTAGCGTCTGGTGTTTGTTTGTTTGAAGCCGTA
CGTCAGCGTTCGTTACAAGAGTAA

Upstream 100 bases:

>100_bases
GTACGCGCAAGAAAGCCAAAGGCTGAGTAAGTAAACATTCGATGATGAGGCAACGTTGCCTCATTAGCTCCACTAATTTG
TAATAGAAAGAAGCGATAAC

Downstream 100 bases:

>100_bases
ATAGTCTATAAAACAACTAGCTAAGAGTGAGGGTAATGATCATTTCATTGCCTTCTTTTTTTATACTAAATTAACAAAAA
TATTTTTGTGATTTAGGTAT

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSNEFLFGIHATKAVLERDPARFIEIFVLKGREDDRLLPIITELGELGFKIQELPRKTLDDKAKGANHQGIIARVTPAKQ
LNERDLDDIMDKTENPLFLVLDGVTDPHNLGACLRNADGAGVAAVIVPKDKSASMTATVSKVACGAAEMVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATKDVFQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVTSLNVSVASGVCLFEAV
RQRSLQE

Sequences:

>Translated_247_residues
MSNEFLFGIHATKAVLERDPARFIEIFVLKGREDDRLLPIITELGELGFKIQELPRKTLDDKAKGANHQGIIARVTPAKQ
LNERDLDDIMDKTENPLFLVLDGVTDPHNLGACLRNADGAGVAAVIVPKDKSASMTATVSKVACGAAEMVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATKDVFQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVTSLNVSVASGVCLFEAV
RQRSLQE
>Mature_246_residues
SNEFLFGIHATKAVLERDPARFIEIFVLKGREDDRLLPIITELGELGFKIQELPRKTLDDKAKGANHQGIIARVTPAKQL
NERDLDDIMDKTENPLFLVLDGVTDPHNLGACLRNADGAGVAAVIVPKDKSASMTATVSKVACGAAEMVPLVRVTNLART
MRALQEKGVWFVGTAGEATKDVFQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVTSLNVSVASGVCLFEAVR
QRSLQE

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=205, Percent_Identity=31.219512195122, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=67.7685950413223, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1788935, Length=144, Percent_Identity=32.6388888888889, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790083, Length=160, Percent_Identity=31.25, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=264, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=254, Percent_Identity=28.3464566929134, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26699; Mature: 26568

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEFLFGIHATKAVLERDPARFIEIFVLKGREDDRLLPIITELGELGFKIQELPRKTLD
CCCCEEEEHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
DKAKGANHQGIIARVTPAKQLNERDLDDIMDKTENPLFLVLDGVTDPHNLGACLRNADGA
HHHCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSASMTATVSKVACGAAEMVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
KDVFQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVTSLNVSVASGVCLFEAV
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
RQRSLQE
HHHHCCC
>Mature Secondary Structure 
SNEFLFGIHATKAVLERDPARFIEIFVLKGREDDRLLPIITELGELGFKIQELPRKTLD
CCCEEEEHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
DKAKGANHQGIIARVTPAKQLNERDLDDIMDKTENPLFLVLDGVTDPHNLGACLRNADGA
HHHCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSASMTATVSKVACGAAEMVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
KDVFQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVTSLNVSVASGVCLFEAV
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
RQRSLQE
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]