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The map label for this gene is pitA [C]

Identifier: 209696090

GI number: 209696090

Start: 2904591

End: 2905877

Strand: Direct

Name: pitA [C]

Synonym: VSAL_I2682

Alternate gene names: 209696090

Gene position: 2904591-2905877 (Clockwise)

Preceding gene: 209696089

Following gene: 209696091

Centisome position: 87.35

GC content: 43.82

Gene sequence:

>1287_bases
ATGGATATTCTTGCTAACTACGGCACGACCCTAATTATAGTTGCGGCCTTCTTCGGTCTTCTAATGGCTATTGGTATTGG
TGCTAATGATGTAGCCAACGCGATGGGCACTTCTGTAGGTTCTAAAGCGTTAACAGTAAAACAAGCAATTATCATTGCTA
TGATCTTCGAATTTGCAGGGGCGTACCTTGCTGGTGGTGAGGTTACAGATACAATTCGTAAAGGGGTAATTGATACCTCT
CTTTTCGCTCATCAACCTGAAGTTTTAGTTTATGGCATGATGTCAGCGCTTCTTGCCGCTGGTACTTGGTTATTACTTGC
TTCTTACATGGGTTGGCCGGTATCAACAACGCACTCAATTATCGGTGCTATCATCGGTTTTGCTTGTGTTGCTGTTGGTA
CGGAAGCGGTTAATTGGAGTTCTGTGCAAGGTATTGTGGGAAGTTGGTTAATCACGCCTCTCATATCCGGTATTTTTGCC
TATATGATTTTCATTAGTGCTCAGCGATTAATCTTCGATACTGATAAACCATTGATGAACGCAAAACGTTTTGTTCCTGT
TTACATGTTCATTACGACAATGGTCATTGCACTTGTTACGATCAAGAAAGGTCTAAAACACGTTGGCTTACACCTATCAA
GCGGCGAAGCATGGCTAGCCTCTATTGCAGTCTCTGCATTAGTCATGGTTGGTGGGTATCTTTATATTTCTCGTAAATTT
GCAAATACAGTAAATGATGAGAAAGACAGCCGTGGCTTTACTGGCGTAGAAAGTATCTTTAGTACGTTAATGGTAATTAC
AGCCTGTGCGATGGCATTTGCTCACGGTTCTAACGATGTTGCCAATGCGATCGGTCCATTGTCTGCGGTTGTTTCAACCA
TTGAAAACATGGGTCAAATTACCTCTCAAACCTCTATTGCTTGGTGGATCCTTCCGCTAGGTGGTATTGGTATCGTTGTT
GGTCTTGCAACCTTAGGTCATAAAGTAATGGCTACCGTTGGTACTGGTATTACAGAACTGACTCCTAGCCGTGGCTTTGC
TGCACAGTTAGCAACGGCTTCGACTGTTGTATTAGCATCAGGTACTGGTCTTCCAATCTCAACAACACAAACATTGGTTG
GTGCGGTTCTTGGGGTAGGCTTTGCTCGTGGTATTGGCGCACTAAACTTAGGTGTAGTACGTAACATTGTTGCCTCTTGG
GTTGTTACCCTACCAGCTGGTGCGTTATTGGCGGTCGTTTTCTACTACGCAATACAAGCCATCTTCGGTATCGGCGCTCC
AGTATAA

Upstream 100 bases:

>100_bases
GTGGGTTGGTGGCATTGCCGACCAAGCACAACGTGTTGGTTCTCGTTTAGAACTGATGTTATCTCGCTCATAAGTTTGTG
ATTCAGTAACAGGTACTACA

Downstream 100 bases:

>100_bases
GCTTAATCAATTTTGAAACAATTGCACAAAGAGGAGAGTAAAATCTCCTCTTTTCTTGCATCTGGGCTTAAATATTTTTA
GTATCTATACCTTCGTTTGA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDILANYGTTLIIVAAFFGLLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFAHQPEVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVNWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSSGEAWLASIAVSALVMVGGYLYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTIENMGQITSQTSIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYAIQAIFGIGAPV

Sequences:

>Translated_428_residues
MDILANYGTTLIIVAAFFGLLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFAHQPEVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVNWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSSGEAWLASIAVSALVMVGGYLYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTIENMGQITSQTSIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYAIQAIFGIGAPV
>Mature_428_residues
MDILANYGTTLIIVAAFFGLLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFAHQPEVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVNWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSSGEAWLASIAVSALVMVGGYLYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTIENMGQITSQTSIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYAIQAIFGIGAPV

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=191, Percent_Identity=40.3141361256544, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI31543630, Length=191, Percent_Identity=41.3612565445026, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25146401, Length=461, Percent_Identity=35.3579175704989, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71981576, Length=452, Percent_Identity=36.7256637168142, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17536725, Length=497, Percent_Identity=27.5653923541247, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17539280, Length=208, Percent_Identity=42.3076923076923, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17557328, Length=507, Percent_Identity=27.4161735700197, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566716, Length=243, Percent_Identity=34.5679012345679, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319773, Length=164, Percent_Identity=40.8536585365854, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356511, Length=259, Percent_Identity=36.2934362934363, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 44349; Mature: 44349

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILANYGTTLIIVAAFFGLLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIDTSLFAHQPEVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSI
CHHCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVAVGTEAVNWSSVQGIVGSWLITPLISGIFAYMIFISAQRLIFDTDKPLMN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSSGEAWLASIAVSALVMVGGYLYISRKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTIENMGQI
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TSQTSIAWWILPLGGIGIVVGLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLAS
HCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEEC
GTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASWVVTLPAGALLAVVFYYAIQA
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IFGIGAPV
HHHCCCCC
>Mature Secondary Structure
MDILANYGTTLIIVAAFFGLLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIDTSLFAHQPEVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSI
CHHCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVAVGTEAVNWSSVQGIVGSWLITPLISGIFAYMIFISAQRLIFDTDKPLMN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSSGEAWLASIAVSALVMVGGYLYISRKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTIENMGQI
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TSQTSIAWWILPLGGIGIVVGLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLAS
HCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEEC
GTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASWVVTLPAGALLAVVFYYAIQA
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
IFGIGAPV
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]