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The map label for this gene is petB [H]

Identifier: 209696069

GI number: 209696069

Start: 2878821

End: 2880089

Strand: Reverse

Name: petB [H]

Synonym: VSAL_I2661

Alternate gene names: 209696069

Gene position: 2880089-2878821 (Counterclockwise)

Preceding gene: 209696070

Following gene: 209696068

Centisome position: 86.61

GC content: 39.01

Gene sequence:

>1269_bases
ATGCAACCATTACTTGATTGGGTAGAAAAACGTCTACCTGCAATGAATGCCTATAAAAAGCATTTATCTGAATATCCAAT
GCCGAAAAACTTTAACTTTTGGTACTTTTTTGGCTCATTAGCAATGCTTGTACTCGTTAATCAGTTAATTACGGGTATTT
GGTTAACAATGAATTATGTTCCTTCTGGAGAAGGTGCATTTGCTTCTGTTGAATACATCATGCGTGATGTCGATTACGGT
TGGCTATTACGTTATATGCACTCCACTGGGGCGTCAGCTTTCTTTGTTGTGGTTTATCTTCATATGTACCGCGGTTTAAT
TTACGGCTCTTACCAGAAGCCTCGTGAACTGTTGTGGTTATTTGGTATGTTGATTTTCTTAGTGTTAATGGCTGAAGCTT
TCATGGGGTATTTATTACCATGGGGACAAATGTCTTACTGGGGAGCTCAAGTTATTATCTCTCTGTTTGGTGCTATCCCT
GTTATTGGTGATGACCTAACACTGTGGATCCGTGGTGATTATGTTATCTCGGGTGCGACGCTCAACCGTTTCTTTGCCTT
ACACGTTATCGCTTTGCCGATTGTGTTATTGCTTTTAGTTGTTCTGCATGTTCTAGCTTTGCATGAAGTCGGGTCAAACA
ACCCTGATGGTATTGAACTTAAAAAACCAAAAGCGAAAGATGATAAGGGTGAGCATAAAACTCAATTTAAATTCCATGAG
TATTATACGAAGAAGTACAACATTATTGATTCTGTTCCTTTCCATCCTTATGGGACGGTAAAAGATATGATCGGTGTTGC
TGGCTTCTTATTCTTCTTTTGCTATGTATTGTTCTTTAATCCTGAAATGGGTGGTTACTTCTTAGAAGGACCAAACTTTG
AAGCCGCAAATCCACTGAAAACGCCAGAGCATATTGCCCCTGTGTGGTACTTCACTCCATTTTATGCAATCTTGCGTGCC
GTTCCTGATAAGTTAATCGGTGTTGCAGCAATGGGTGCTTCAATTGTTGTGCTGTTCCTTCTTCCTTGGTTGGATCGCTG
TAAAGTAAGGTCGTACCGTTACCGTAGTAAGTTGCATTTACTTAATATTGCGCAGTTTACTGTGAGCTTTATTGCTCTTG
GTGTTTTGGGTTCATTACCTGCAACGTCACTGTATACATTGTTAGCTCAGATATTCAGCTTAACTTACTTTATGTTCTTT
GTTCTTTTGTTTGTTTACAGTAAAAATGAAAAAACAAAACCATTACCAGAAAGGGTGACATTCAAATGA

Upstream 100 bases:

>100_bases
TCCAAGGCGTACCTGCACCATTAAATCTCGTGGTGCCAAAACACATGTACCTTAGTGATACTCGTATCCTAATTGGGGTT
GATGACGGAGGGGAAGCCTA

Downstream 100 bases:

>100_bases
AAAAGTTGATTTTAGTACTTTTTGCTTTGTTGCCATCAATGGCAATGGCTGCAGGGTCGGGAGCGCCATTAGATAGCGCA
AATAATGATATTCGTGATCA

Product: ubiquinol--cytochrome c reductase, cytochrome B

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQPLLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYFFGSLAMLVLVNQLITGIWLTMNYVPSGEGAFASVEYIMRDVDYG
WLLRYMHSTGASAFFVVVYLHMYRGLIYGSYQKPRELLWLFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLVVLHVLALHEVGSNNPDGIELKKPKAKDDKGEHKTQFKFHE
YYTKKYNIIDSVPFHPYGTVKDMIGVAGFLFFFCYVLFFNPEMGGYFLEGPNFEAANPLKTPEHIAPVWYFTPFYAILRA
VPDKLIGVAAMGASIVVLFLLPWLDRCKVRSYRYRSKLHLLNIAQFTVSFIALGVLGSLPATSLYTLLAQIFSLTYFMFF
VLLFVYSKNEKTKPLPERVTFK

Sequences:

>Translated_422_residues
MQPLLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYFFGSLAMLVLVNQLITGIWLTMNYVPSGEGAFASVEYIMRDVDYG
WLLRYMHSTGASAFFVVVYLHMYRGLIYGSYQKPRELLWLFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLVVLHVLALHEVGSNNPDGIELKKPKAKDDKGEHKTQFKFHE
YYTKKYNIIDSVPFHPYGTVKDMIGVAGFLFFFCYVLFFNPEMGGYFLEGPNFEAANPLKTPEHIAPVWYFTPFYAILRA
VPDKLIGVAAMGASIVVLFLLPWLDRCKVRSYRYRSKLHLLNIAQFTVSFIALGVLGSLPATSLYTLLAQIFSLTYFMFF
VLLFVYSKNEKTKPLPERVTFK
>Mature_422_residues
MQPLLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYFFGSLAMLVLVNQLITGIWLTMNYVPSGEGAFASVEYIMRDVDYG
WLLRYMHSTGASAFFVVVYLHMYRGLIYGSYQKPRELLWLFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLVVLHVLALHEVGSNNPDGIELKKPKAKDDKGEHKTQFKFHE
YYTKKYNIIDSVPFHPYGTVKDMIGVAGFLFFFCYVLFFNPEMGGYFLEGPNFEAANPLKTPEHIAPVWYFTPFYAILRA
VPDKLIGVAAMGASIVVLFLLPWLDRCKVRSYRYRSKLHLLNIAQFTVSFIALGVLGSLPATSLYTLLAQIFSLTYFMFF
VLLFVYSKNEKTKPLPERVTFK

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=399, Percent_Identity=40.3508771929825, Blast_Score=261, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6226529, Length=385, Percent_Identity=40, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6226532, Length=274, Percent_Identity=40.1459854014599, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6226531, Length=163, Percent_Identity=48.4662576687117, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6226530, Length=152, Percent_Identity=46.7105263157895, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 48470; Mature: 48470

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYFFGSLAMLVLVNQLITGIWLTMNYV
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PSGEGAFASVEYIMRDVDYGWLLRYMHSTGASAFFVVVYLHMYRGLIYGSYQKPRELLWL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIPVIGDDLTLWIRGDYVISGAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCEEEECHH
LNRFFALHVIALPIVLLLLVVLHVLALHEVGSNNPDGIELKKPKAKDDKGEHKTQFKFHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHH
YYTKKYNIIDSVPFHPYGTVKDMIGVAGFLFFFCYVLFFNPEMGGYFLEGPNFEAANPLK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
TPEHIAPVWYFTPFYAILRAVPDKLIGVAAMGASIVVLFLLPWLDRCKVRSYRYRSKLHL
CHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIAQFTVSFIALGVLGSLPATSLYTLLAQIFSLTYFMFFVLLFVYSKNEKTKPLPERVT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FK
CC
>Mature Secondary Structure
MQPLLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYFFGSLAMLVLVNQLITGIWLTMNYV
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PSGEGAFASVEYIMRDVDYGWLLRYMHSTGASAFFVVVYLHMYRGLIYGSYQKPRELLWL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIPVIGDDLTLWIRGDYVISGAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCEEEECHH
LNRFFALHVIALPIVLLLLVVLHVLALHEVGSNNPDGIELKKPKAKDDKGEHKTQFKFHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHH
YYTKKYNIIDSVPFHPYGTVKDMIGVAGFLFFFCYVLFFNPEMGGYFLEGPNFEAANPLK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
TPEHIAPVWYFTPFYAILRAVPDKLIGVAAMGASIVVLFLLPWLDRCKVRSYRYRSKLHL
CHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIAQFTVSFIALGVLGSLPATSLYTLLAQIFSLTYFMFFVLLFVYSKNEKTKPLPERVT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA