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The map label for this gene is mraW

Identifier: 209696060

GI number: 209696060

Start: 2870831

End: 2871781

Strand: Reverse

Name: mraW

Synonym: VSAL_I2652

Alternate gene names: 209696060

Gene position: 2871781-2870831 (Counterclockwise)

Preceding gene: 209696061

Following gene: 209696059

Centisome position: 86.37

GC content: 40.69

Gene sequence:

>951_bases
ATGTCTGAACAATTTCAACACGTATCCGTTCTTCTTCATGAATCTATTGATGGTTTAGCGATTAAACCTGATGGTATCTA
TATTGATGGTACTTTTGGCCGCGGTGGTCATAGTCGTCAAATTTTATCTCGATTAGGTGAAAACGGACGTTTGTACAGTA
TTGACCGTGATCCTCAAGCAATTGCTGAAGCGAAAACCATTACGGATCCTAGATTCAACATTATTCATGGACCATTCTCT
GGTCTAAAAGGGTATGTCGAAGAGTTAGGTTTAGTGGGACAAATTGATGGTGTTTTACTTGATTTAGGTGTGTCATCACC
ACAACTTGATGATGCAGACCGTGGTTTTAGCTTCATGAAAGATGGCCCATTAGATATGCGCATGGATCCAACCTCTGGAA
TGCCGGTATCTCAATGGTTATTGACTGCCGATGTTGAAGATATTACTTGGGTTATTCGTGAGTTTGGCGAAGATAAGCAC
GCATGGCGAATTGCTAAAGGTATTGTTGCTTACCGTGATAACGAAGAGAATGAGCCGTTAACTCGTACTGGTCAACTCGC
TAAATTAATTTCTGATGTTGCGCCTAAGAATTTTAAAGAGAAAAAGCATCCTGCGACTCGTACTTTCCAAGCATTCCGCA
TTTATATTAATAGTGAATTGGATGAGATTGATACGGCGTTAAATGGCGCTCTTGATGTGTTTGCTGAGGGTGGACGTTTG
TCTGTGATTAGTTTTCATTCATTAGAAGATCGCATGGTTAAGCACTTTATTCGTAAAGAAAGCAGAGGTCCGCAAGTCCC
TCATGGTATTCCTCTGACTGAAAACCAAATTAAAGCATTAGGCAGTGCAAAAATGAAGCCTATTGGTAAAGCCATTAAAC
CTACCGTTAATGAAGTTGATGTTAATGTTCGCTCGCGTAGTTCTGTTCTACGTATCGCTGAGCGCTTATGA

Upstream 100 bases:

>100_bases
GAATTATCACTAATTTGACACACACCACTGTATTTTTAGTTTACACTTCTAGCTAAAATTCAGTCGCTTATTCTCATTAT
TACGGTAGTAAATACTCACT

Downstream 100 bases:

>100_bases
GCCACAAAGCATCATCACAATCCAGTTTAACTAAAATGATAGGTCTAGACTTAATAGGTGTAGGCCGTTTTCACTCGATC
TTATTAATGTTTCTTTTTAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSRLGENGRLYSIDRDPQAIAEAKTITDPRFNIIHGPFS
GLKGYVEELGLVGQIDGVLLDLGVSSPQLDDADRGFSFMKDGPLDMRMDPTSGMPVSQWLLTADVEDITWVIREFGEDKH
AWRIAKGIVAYRDNEENEPLTRTGQLAKLISDVAPKNFKEKKHPATRTFQAFRIYINSELDEIDTALNGALDVFAEGGRL
SVISFHSLEDRMVKHFIRKESRGPQVPHGIPLTENQIKALGSAKMKPIGKAIKPTVNEVDVNVRSRSSVLRIAERL

Sequences:

>Translated_316_residues
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSRLGENGRLYSIDRDPQAIAEAKTITDPRFNIIHGPFS
GLKGYVEELGLVGQIDGVLLDLGVSSPQLDDADRGFSFMKDGPLDMRMDPTSGMPVSQWLLTADVEDITWVIREFGEDKH
AWRIAKGIVAYRDNEENEPLTRTGQLAKLISDVAPKNFKEKKHPATRTFQAFRIYINSELDEIDTALNGALDVFAEGGRL
SVISFHSLEDRMVKHFIRKESRGPQVPHGIPLTENQIKALGSAKMKPIGKAIKPTVNEVDVNVRSRSSVLRIAERL
>Mature_315_residues
SEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSRLGENGRLYSIDRDPQAIAEAKTITDPRFNIIHGPFSG
LKGYVEELGLVGQIDGVLLDLGVSSPQLDDADRGFSFMKDGPLDMRMDPTSGMPVSQWLLTADVEDITWVIREFGEDKHA
WRIAKGIVAYRDNEENEPLTRTGQLAKLISDVAPKNFKEKKHPATRTFQAFRIYINSELDEIDTALNGALDVFAEGGRLS
VISFHSLEDRMVKHFIRKESRGPQVPHGIPLTENQIKALGSAKMKPIGKAIKPTVNEVDVNVRSRSSVLRIAERL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=36.4705882352941, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=38.6934673366834, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=62.9746835443038, Blast_Score=405, Evalue=1e-114,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=36.969696969697, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=36.969696969697, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ALISL (B6ELI3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263990.1
- ProteinModelPortal:   B6ELI3
- GeneID:   6988570
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2652
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35122; Mature: 34991

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSRLGENGRLYSIDRDPQA
CCCHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAKTITDPRFNIIHGPFSGLKGYVEELGLVGQIDGVLLDLGVSSPQLDDADRGFSFMK
HHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHC
DGPLDMRMDPTSGMPVSQWLLTADVEDITWVIREFGEDKHAWRIAKGIVAYRDNEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCC
TRTGQLAKLISDVAPKNFKEKKHPATRTFQAFRIYINSELDEIDTALNGALDVFAEGGRL
HHHHHHHHHHHHHCCCCCHHHCCCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCEE
SVISFHSLEDRMVKHFIRKESRGPQVPHGIPLTENQIKALGSAKMKPIGKAIKPTVNEVD
EEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCHHEE
VNVRSRSSVLRIAERL
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQFQHVSVLLHESIDGLAIKPDGIYIDGTFGRGGHSRQILSRLGENGRLYSIDRDPQA
CCHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAKTITDPRFNIIHGPFSGLKGYVEELGLVGQIDGVLLDLGVSSPQLDDADRGFSFMK
HHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHC
DGPLDMRMDPTSGMPVSQWLLTADVEDITWVIREFGEDKHAWRIAKGIVAYRDNEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCC
TRTGQLAKLISDVAPKNFKEKKHPATRTFQAFRIYINSELDEIDTALNGALDVFAEGGRL
HHHHHHHHHHHHHCCCCCHHHCCCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCEE
SVISFHSLEDRMVKHFIRKESRGPQVPHGIPLTENQIKALGSAKMKPIGKAIKPTVNEVD
EEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCHHEE
VNVRSRSSVLRIAERL
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA