Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 209696036

GI number: 209696036

Start: 2843249

End: 2843800

Strand: Reverse

Name: ampD [H]

Synonym: VSAL_I2627

Alternate gene names: 209696036

Gene position: 2843800-2843249 (Counterclockwise)

Preceding gene: 209696044

Following gene: 209696035

Centisome position: 85.52

GC content: 42.39

Gene sequence:

>552_bases
ATGAGATTCACGTTATCAACAACGCATTGGATCAATGAAGCGAAACACGTTGTCTCGCCATTTTATGACGAACGCTCCGA
TGACTTAGACATATCTTTACTTGTGGTACACAACATCAGCTTACCGCCGGGTCAGTTTGGTGGCCCTTATATTGAACAAT
TATTTACCGGTACTTTAAATCCGAACGAACACCCATTCTTTAACGTTATCCATCAATTTAAAGTGTCTGCCCATTGTTTG
ATTCGTCGAGATGGGCGTGTTATTCAATTTGTGCCCTTTAATTCTCGAGCGTGGCATGCGGGAGCATCGTCGTTTGCGGG
CAGATCGAAATGCAATGATTACAGTATCGGCATTGAATTAGAAGGAACGGATTCAGTGGCCTATACTGAGGAGCAGTATC
AATCACTGGCTTCATTAACAAAGGTGATACAACAGCATTACCCGTTAGTAAAGGATGATCGTATTACGGGTCATCAGTAT
ATAGCGCCGCTTAGAAAGACGGATCCGGGGCTGTCTTTTCATTGGGAAAAGTATAATAAGTTAAGAAAATAG

Upstream 100 bases:

>100_bases
GAATTATTTGAAATAAGTTGATTCACACAATAATACTCGCGCTAGAGCAGAATTTCATCATAAGATACCTAAAGTTACTT
CAATTTATGTAGGAATAGAT

Downstream 100 bases:

>100_bases
GGGAGTTACCTCTGAAAAGGTATATACATTGTTAGGGGTTGTGGCACAATCTTTTTGCCTCACAAATCAAATTGGTCTGA
CCAATTATTTCAAATATGTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MRFTLSTTHWINEAKHVVSPFYDERSDDLDISLLVVHNISLPPGQFGGPYIEQLFTGTLNPNEHPFFNVIHQFKVSAHCL
IRRDGRVIQFVPFNSRAWHAGASSFAGRSKCNDYSIGIELEGTDSVAYTEEQYQSLASLTKVIQQHYPLVKDDRITGHQY
IAPLRKTDPGLSFHWEKYNKLRK

Sequences:

>Translated_183_residues
MRFTLSTTHWINEAKHVVSPFYDERSDDLDISLLVVHNISLPPGQFGGPYIEQLFTGTLNPNEHPFFNVIHQFKVSAHCL
IRRDGRVIQFVPFNSRAWHAGASSFAGRSKCNDYSIGIELEGTDSVAYTEEQYQSLASLTKVIQQHYPLVKDDRITGHQY
IAPLRKTDPGLSFHWEKYNKLRK
>Mature_183_residues
MRFTLSTTHWINEAKHVVSPFYDERSDDLDISLLVVHNISLPPGQFGGPYIEQLFTGTLNPNEHPFFNVIHQFKVSAHCL
IRRDGRVIQFVPFNSRAWHAGASSFAGRSKCNDYSIGIELEGTDSVAYTEEQYQSLASLTKVIQQHYPLVKDDRITGHQY
IAPLRKTDPGLSFHWEKYNKLRK

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=58.7209302325581, Blast_Score=223, Evalue=8e-60,
Organism=Escherichia coli, GI1787092, Length=120, Percent_Identity=39.1666666666667, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21006; Mature: 21006

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTLSTTHWINEAKHVVSPFYDERSDDLDISLLVVHNISLPPGQFGGPYIEQLFTGTLN
CEEECCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCC
PNEHPFFNVIHQFKVSAHCLIRRDGRVIQFVPFNSRAWHAGASSFAGRSKCNDYSIGIEL
CCCCHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
EGTDSVAYTEEQYQSLASLTKVIQQHYPLVKDDRITGHQYIAPLRKTDPGLSFHWEKYNK
CCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCCCCEEEHHHHHH
LRK
CCC
>Mature Secondary Structure
MRFTLSTTHWINEAKHVVSPFYDERSDDLDISLLVVHNISLPPGQFGGPYIEQLFTGTLN
CEEECCHHHHHHHHHHHHCCHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCC
PNEHPFFNVIHQFKVSAHCLIRRDGRVIQFVPFNSRAWHAGASSFAGRSKCNDYSIGIEL
CCCCHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
EGTDSVAYTEEQYQSLASLTKVIQQHYPLVKDDRITGHQYIAPLRKTDPGLSFHWEKYNK
CCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCCCCEEEHHHHHH
LRK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]