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The map label for this gene is dksA [H]

Identifier: 209696019

GI number: 209696019

Start: 2821552

End: 2822022

Strand: Direct

Name: dksA [H]

Synonym: VSAL_I2605

Alternate gene names: 209696019

Gene position: 2821552-2822022 (Clockwise)

Preceding gene: 209696018

Following gene: 209696020

Centisome position: 84.85

GC content: 40.98

Gene sequence:

>471_bases
ATGTTTTTAGGAGAAGCTGTAATGCCACAGAGCAATACAAAAAAGACGCTAGGTATTCTAGCTATTGCAGGTGTAGAACC
TTACCAGCCTAAAGCTGGTGAAGAGTACATGGGCCCAGCACAAGTAGAACATTTTACTAAAATTCTAGACGCTTGGCGTA
ATCAACTAAGGGAAGAAGTTGAACGCACAGTTAATCATATGAAAGATGAGGCAGCGAATTTCCCGGATCCAGTAGATCGC
GCCGCTCAAGAAGAAGAATTTAGTTTAGAGCTACGTAATCGCGATCGCGAGCGTCGCCTGATTAAAAAAATTGAAAAAAC
ACTAAGCAAAATCGAAGAAGAAGAATTCGGATTCTGTGATTCTTGTGGTGTTGAAATTGGTATTCGTCGCTTAGAAGCAC
GTCCAACTGCTGACCTTTGTATTGACTGTAAAACACTTGCAGAAATCAAAGAAAAGCAAATGGCAGGTTAA

Upstream 100 bases:

>100_bases
ACGTTCTTGATACTAGATCTCAATTGGTGAAATCAATATTTGATTGCCCCTTTTCTTTCTGTTATAGATAGCGCCCTCAT
TTGGCTAGTGAGAGTCAGAG

Downstream 100 bases:

>100_bases
TACCTATAAAGTAATTAAAAAGGGAGCATTGGCTCCCTTTTTTACGTCTAAATAGTAGAATATGATGAACACTCAGTACA
TAGGACGATTTGCTCCATCA

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFLGEAVMPQSNTKKTLGILAIAGVEPYQPKAGEEYMGPAQVEHFTKILDAWRNQLREEVERTVNHMKDEAANFPDPVDR
AAQEEEFSLELRNRDRERRLIKKIEKTLSKIEEEEFGFCDSCGVEIGIRRLEARPTADLCIDCKTLAEIKEKQMAG

Sequences:

>Translated_156_residues
MFLGEAVMPQSNTKKTLGILAIAGVEPYQPKAGEEYMGPAQVEHFTKILDAWRNQLREEVERTVNHMKDEAANFPDPVDR
AAQEEEFSLELRNRDRERRLIKKIEKTLSKIEEEEFGFCDSCGVEIGIRRLEARPTADLCIDCKTLAEIKEKQMAG
>Mature_156_residues
MFLGEAVMPQSNTKKTLGILAIAGVEPYQPKAGEEYMGPAQVEHFTKILDAWRNQLREEVERTVNHMKDEAANFPDPVDR
AAQEEEFSLELRNRDRERRLIKKIEKTLSKIEEEEFGFCDSCGVEIGIRRLEARPTADLCIDCKTLAEIKEKQMAG

Specific function: Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed [H]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=147, Percent_Identity=80.9523809523809, Blast_Score=252, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR020460
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 17827; Mature: 17827

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLGEAVMPQSNTKKTLGILAIAGVEPYQPKAGEEYMGPAQVEHFTKILDAWRNQLREEV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERTVNHMKDEAANFPDPVDRAAQEEEFSLELRNRDRERRLIKKIEKTLSKIEEEEFGFCD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SCGVEIGIRRLEARPTADLCIDCKTLAEIKEKQMAG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLGEAVMPQSNTKKTLGILAIAGVEPYQPKAGEEYMGPAQVEHFTKILDAWRNQLREEV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERTVNHMKDEAANFPDPVDRAAQEEEFSLELRNRDRERRLIKKIEKTLSKIEEEEFGFCD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SCGVEIGIRRLEARPTADLCIDCKTLAEIKEKQMAG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]