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The map label for this gene is mtnN

Identifier: 209695982

GI number: 209695982

Start: 2757664

End: 2758359

Strand: Direct

Name: mtnN

Synonym: VSAL_I2565

Alternate gene names: 209695982

Gene position: 2757664-2758359 (Clockwise)

Preceding gene: 209695981

Following gene: 209695983

Centisome position: 82.93

GC content: 39.51

Gene sequence:

>696_bases
ATGAAAATCGGTATTATTGGTGCAATGGAACAAGAAGTTGCCATTCTAAAAGAACAAATTAATGGCTTAGCGACAACAAT
CAAAGCTGGTTGTACTTTTCATACAGGCACATTGAATGGTGCTGAAGTCGTTTTACTTCAATCAGGCATTGGTAAAGTAG
CGGCAGCCGTAGGGACAACTCTTCTTATCTCAGATCATAATGTAGATGTTGTTCTTAATACTGGCTCTGCTGGTGGGTTT
GATTCTTCATTGAATCTTGGTGATGTCGTTATTTCTACAGAAGTTCGTCATCATGATGCTGATGTAACTGCATTTGGTTA
CGAGATGGGACAGATGGCACAACAACCAGCTGCTTTTAAAGCAGATGAAAAACTAATGGCAGTCGCAGAAAAAGCATTGA
CTCAAATGGAAGATAAGCATGCTGTACGTGGTTTAATCTGTACTGGTGATGCTTTTGTATGTACGCCTGAGCGCCAAGCA
TTTATTCGCTCTCATTTTCCTTCTGTTATCGCTGTTGAAATGGAAGCGTCAGCCATTGCTCAAACTTGTCATCAATTTAG
TGTTCCATTTGTTGTCGTTCGTGCAATATCTGATGTGGCAGATAAAGAATCACCAATGAGTTTTGAAGAATTCTTGCCTC
TTGCTGCAGAAAGTTCATCAGAAATGGTGATGAAGATGGTTACTTTATTAAAGTAA

Upstream 100 bases:

>100_bases
TCTCACTTTATCCTCTTTTATCTCAAGAAAACTCTTATTCCACCACCTTAAATAGCTATGTTACAATTCAGTTCTATTCG
AACGTTACTGAGATAAACAA

Downstream 100 bases:

>100_bases
TAACATAATAATGGAGTTAACGATTAGTTTGCTCCATTGTCCCTCTATATATAGTTAATACATATATGCTTGATAAACTG
TCTTTCTTCATCGCCGATTA

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTTLLISDHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFKADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQA
FIRSHFPSVIAVEMEASAIAQTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK

Sequences:

>Translated_231_residues
MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTTLLISDHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFKADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQA
FIRSHFPSVIAVEMEASAIAQTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK
>Mature_231_residues
MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTTLLISDHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFKADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQA
FIRSHFPSVIAVEMEASAIAQTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=57.8260869565217, Blast_Score=236, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_ALISL (B6EKZ7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263912.1
- GeneID:   6988169
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2565
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- ProtClustDB:   PRK05584
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 24531; Mature: 24531

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTT
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHCCE
LLISDHNVDVVLNTGSAGGFDSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFK
EEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCC
ADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQAFIRSHFPSVIAVEMEASAIA
CHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCHHHHHHHHCCCCEEEEEEHHHHHH
QTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTT
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCHHHHHHHCCE
LLISDHNVDVVLNTGSAGGFDSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFK
EEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCC
ADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQAFIRSHFPSVIAVEMEASAIA
CHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCHHHHHHHHCCCCEEEEEEHHHHHH
QTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA