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The map label for this gene is grcA

Identifier: 209695971

GI number: 209695971

Start: 2733601

End: 2733978

Strand: Direct

Name: grcA

Synonym: VSAL_I2553

Alternate gene names: 209695971

Gene position: 2733601-2733978 (Clockwise)

Preceding gene: 209695967

Following gene: 209695976

Centisome position: 82.21

GC content: 40.74

Gene sequence:

>378_bases
ATGATCACAGGTATCCAAATTACTAAAGCTGCAAATGACGATCTTCTTAACTCTATCTGGCTACTAGATAGCGAGAAAAA
TGAAGCACGTTGTGTTGTTGCAACTTCAGGCTTTGAAGCTGACCAAGTTATTGCAACAAGCGCACTGGGCGAATACGAAA
GCCGTGACGTTGCTATTGAAAAAGCACCAAAAGTTGAAGGTGGCCAACATCTAAATGTTAACGTTCTGCAACGTGATACA
TTACAAGACGCAGTTAAGCACCCAGAGAAATACCCACAGTTAACTATCCGTGTATCGGGTTATGCTGTACGTTTTAATTC
TCTAACAACAGAGCAACAACAAGACGTTATCGCACGTACTTTTACAGAAACTCTATAA

Upstream 100 bases:

>100_bases
TTTTGACCTATATCAATATACAAAGTAAGGGTATCATGTATATAATGTGACCCATAAGATATTTACAAAATAACCACAAA
GTGGACTAGGAGAAAGTAAA

Downstream 100 bases:

>100_bases
TCTAGACTTTCCTAAATAAAAAACGGCACTCATTGCTCATGAGTGCCGTTTTTCTATATAAAAGCCTCTTCAATACTGAC
TAATTAGTTAGCATTTAAGA

Product: autonomous glycyl radical cofactor GrcA

Products: CoA; pyruvate

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVIATSALGEYESRDVAIEKAPKVEGGQHLNVNVLQRDT
LQDAVKHPEKYPQLTIRVSGYAVRFNSLTTEQQQDVIARTFTETL

Sequences:

>Translated_125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVIATSALGEYESRDVAIEKAPKVEGGQHLNVNVLQRDT
LQDAVKHPEKYPQLTIRVSGYAVRFNSLTTEQQQDVIARTFTETL
>Mature_125_residues
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVIATSALGEYESRDVAIEKAPKVEGGQHLNVNVLQRDT
LQDAVKHPEKYPQLTIRVSGYAVRFNSLTTEQQQDVIARTFTETL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=77.1653543307087, Blast_Score=207, Evalue=2e-55,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=78.6885245901639, Blast_Score=107, Evalue=2e-25,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=77.0491803278689, Blast_Score=106, Evalue=4e-25,
Organism=Escherichia coli, GI1787044, Length=57, Percent_Identity=45.6140350877193, Blast_Score=63, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRCA_ALISL (B6EKY5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263901.1
- GeneID:   6988894
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2553
- HOGENOM:   HBG297207
- OMA:   GQFEYRE
- ProtClustDB:   PRK11127
- HAMAP:   MF_00806
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140
- PIRSF:   PIRSF000378

Pfam domain/function: PF01228 Gly_radical

EC number: 2.3.1.54

Molecular weight: Translated: 13849; Mature: 13849

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVIATSALGEYESRDVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
KAPKVEGGQHLNVNVLQRDTLQDAVKHPEKYPQLTIRVSGYAVRFNSLTTEQQQDVIART
CCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTETL
HHHCC
>Mature Secondary Structure
MITGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVIATSALGEYESRDVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
KAPKVEGGQHLNVNVLQRDTLQDAVKHPEKYPQLTIRVSGYAVRFNSLTTEQQQDVIART
CCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTETL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA