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The map label for this gene is ygfZ [C]

Identifier: 209695953

GI number: 209695953

Start: 2716433

End: 2717389

Strand: Direct

Name: ygfZ [C]

Synonym: VSAL_I2535

Alternate gene names: 209695953

Gene position: 2716433-2717389 (Clockwise)

Preceding gene: 209695950

Following gene: 209695954

Centisome position: 81.69

GC content: 39.29

Gene sequence:

>957_bases
ATGATGAGCACATTGTTTCCAAAACTATCAATTAAAAAAGAAGACACATTACCTGCGCTTGCTATTTCAGAGCTTAATGA
TTGGGCTTTGATCACAATGATCGGTAATGATAAAAAGTCCTACCTTCAGGGACAAGTAACCGCTGATGTAGTAACTCTCC
CACAGGATGACATTACCTTTGGTGGCCATTGTGATGCAAAAGGTAAATTATGGAGTATTTTCCAACTGTTCAACCATAAC
GATGGTTATGCTTTATTTCAGCGAAGAAGTGCCATTGAAACAGAACTCACTGAAATAAAAAAATACTCTGTATTTTCTAA
AGTTGATATCGCGGTTGGTGATGACGTTTTATTGGGGATTAGCGGTAAAAAGGCAACAGATTGGGTAAACAAACACACAA
CAACTGACGCGAATGTTCGTGCTTGCGAGCTTGGAACGTTTGCTAAAATAAGTGAAACTCAATGGTTACTCGTCACCACA
CCAGAACACAAAAAAAATATAATAAACCAAGAAAGTAACACGGTGCTATGTGACGAATCATTATGGTCATTACACACAAT
TCAACATGGTTTACCTCAGCTAGATAATGCATTAAGTAACGCTCACATTCCACAAGCCATGAATTTACAAGCTGTCGGTG
GTATCAGCTTTGCTAAAGGTTGCTACACAGGACAAGAAACCGTTGCTCGAGCAAAATATCGAGGCATTAATAAACGAGCA
ATGTACCTATTATCAGGTCAATCCAATTCAATACCTGTCGCAGGTGATGCAATTGAACGTAGTGTGGGTGAAAACTGGCG
AAAAGGAGGCACTATCGTATCGGCTTACCGTTTTGAAGATGGTTATACGTTAGCACTTGCTATTTTACCTAACGACCTAG
AGGAAGGTACCCAGTTCAAACTCCAAGATTCAATTTGGAACAAAGGCAATCAACCTTACTCATTAGATGAAGAGTAA

Upstream 100 bases:

>100_bases
AAGATGAAGAAGATGAGACATCAAACTGATGTTTCTTTTACTGACATTTAAAATCTAGGCATTTACTATAAAGAGAATTA
CTCAACAAAATAAGAATTTA

Downstream 100 bases:

>100_bases
ATTAAATCACTCATACTTTGCGTTTCAATAATGAGTATTTAATACATAGTTACATTGCTACTTTGAAGTAACCTGCATAA
ATCAAGACACAAGAATGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MMSTLFPKLSIKKEDTLPALAISELNDWALITMIGNDKKSYLQGQVTADVVTLPQDDITFGGHCDAKGKLWSIFQLFNHN
DGYALFQRRSAIETELTEIKKYSVFSKVDIAVGDDVLLGISGKKATDWVNKHTTTDANVRACELGTFAKISETQWLLVTT
PEHKKNIINQESNTVLCDESLWSLHTIQHGLPQLDNALSNAHIPQAMNLQAVGGISFAKGCYTGQETVARAKYRGINKRA
MYLLSGQSNSIPVAGDAIERSVGENWRKGGTIVSAYRFEDGYTLALAILPNDLEEGTQFKLQDSIWNKGNQPYSLDEE

Sequences:

>Translated_318_residues
MMSTLFPKLSIKKEDTLPALAISELNDWALITMIGNDKKSYLQGQVTADVVTLPQDDITFGGHCDAKGKLWSIFQLFNHN
DGYALFQRRSAIETELTEIKKYSVFSKVDIAVGDDVLLGISGKKATDWVNKHTTTDANVRACELGTFAKISETQWLLVTT
PEHKKNIINQESNTVLCDESLWSLHTIQHGLPQLDNALSNAHIPQAMNLQAVGGISFAKGCYTGQETVARAKYRGINKRA
MYLLSGQSNSIPVAGDAIERSVGENWRKGGTIVSAYRFEDGYTLALAILPNDLEEGTQFKLQDSIWNKGNQPYSLDEE
>Mature_318_residues
MMSTLFPKLSIKKEDTLPALAISELNDWALITMIGNDKKSYLQGQVTADVVTLPQDDITFGGHCDAKGKLWSIFQLFNHN
DGYALFQRRSAIETELTEIKKYSVFSKVDIAVGDDVLLGISGKKATDWVNKHTTTDANVRACELGTFAKISETQWLLVTT
PEHKKNIINQESNTVLCDESLWSLHTIQHGLPQLDNALSNAHIPQAMNLQAVGGISFAKGCYTGQETVARAKYRGINKRA
MYLLSGQSNSIPVAGDAIERSVGENWRKGGTIVSAYRFEDGYTLALAILPNDLEEGTQFKLQDSIWNKGNQPYSLDEE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=329, Percent_Identity=42.5531914893617, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_ALISL (B6EKN9)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263883.1
- GeneID:   6987802
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2535
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   CLSK874810
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR013977
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: NA

Molecular weight: Translated: 35204; Mature: 35204

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTLFPKLSIKKEDTLPALAISELNDWALITMIGNDKKSYLQGQVTADVVTLPQDDITF
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHCCCEEEEEEECCCCCCEE
GGHCDAKGKLWSIFQLFNHNDGYALFQRRSAIETELTEIKKYSVFSKVDIAVGDDVLLGI
CCCCCCCCCCEEEHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEE
SGKKATDWVNKHTTTDANVRACELGTFAKISETQWLLVTTPEHKKNIINQESNTVLCDES
CCCCHHHHHHCCCCCCCCEEEEECCCCEEECCCEEEEEECCHHHHHHHCCCCCEEEECCH
LWSLHTIQHGLPQLDNALSNAHIPQAMNLQAVGGISFAKGCYTGQETVARAKYRGINKRA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHCCCCCEE
MYLLSGQSNSIPVAGDAIERSVGENWRKGGTIVSAYRFEDGYTLALAILPNDLEEGTQFK
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCEEE
LQDSIWNKGNQPYSLDEE
EEHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMSTLFPKLSIKKEDTLPALAISELNDWALITMIGNDKKSYLQGQVTADVVTLPQDDITF
CCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHCCCEEEEEEECCCCCCEE
GGHCDAKGKLWSIFQLFNHNDGYALFQRRSAIETELTEIKKYSVFSKVDIAVGDDVLLGI
CCCCCCCCCCEEEHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEE
SGKKATDWVNKHTTTDANVRACELGTFAKISETQWLLVTTPEHKKNIINQESNTVLCDES
CCCCHHHHHHCCCCCCCCEEEEECCCCEEECCCEEEEEECCHHHHHHHCCCCCEEEECCH
LWSLHTIQHGLPQLDNALSNAHIPQAMNLQAVGGISFAKGCYTGQETVARAKYRGINKRA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHCCCCCEE
MYLLSGQSNSIPVAGDAIERSVGENWRKGGTIVSAYRFEDGYTLALAILPNDLEEGTQFK
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCEEE
LQDSIWNKGNQPYSLDEE
EEHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA