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The map label for this gene is nadB [H]

Identifier: 209695950

GI number: 209695950

Start: 2713911

End: 2715527

Strand: Direct

Name: nadB [H]

Synonym: VSAL_I2532

Alternate gene names: 209695950

Gene position: 2713911-2715527 (Clockwise)

Preceding gene: 209695938

Following gene: 209695953

Centisome position: 81.62

GC content: 42.3

Gene sequence:

>1617_bases
ATGAACATAACCAATGAACACAGTTGTGACGTCTTAGTCATAGGTAGTGGTGCTGCTGGTCTTTCACTTGCACTTAGAGT
CGCACCACATGGAAAGGTGATCGTTTTAAGTAAGGGCCCACGTAATGAAGGGTCTACCTACTACGCTCAAGGTGGCATTG
CTGCCGTATTTGATGAAAGTGATACTATAGAATCTCATGTCGATGACACCTTAATTGCAGGCGCTCATATTTGCGATAAA
GACGTTGTTACCTTCATTGCAGAAAATGCTAAGCATTGTGTTCAATGGTTAATTGATGGCGGTGTTCCTTTTGATCATGA
TGATGAAAAAGAAACCGACATATCTCACCCGAGGTTCCATTTAACTCGAGAAGGTGGCCATAGTCATCGTCGCATTCTTC
ACGCGGCAGATGCAACGGGTAAAGCAATGCAAACGTCGTTGCAAGATAACGTCGTTAATCACCCTAACATTACAATTTTT
GAACGTCATAACGCATTAGATTTAATTACTGAAGACAAAATAGGCGGTGACATTGATAAAGTCGTCGGTGCTTATATTTG
GAACCGTGACGCTGAACATGTCGAAACCGTTCGTGCCAAATTTGTGGTTATAGCCACTGGTGGTGCTTCTAAAGTTTATC
AATATACGTCGAATCCTGACGTCTCTTCTGGTGATGGCATTGCAATGGCTTGGCGTGCGGGGTGCCGTGTCGCGAATTTA
GAATTTAATCAATTTCATCCAACGTGCCTATTTCATCCAGATGCCCGTAATTTTTTAATGTCTGAAGCATTACGTGGTGA
AGGCGCTTACTTACGTCGTCCTGATGGATCTCGTTTCATGTTGGATTTCGATGAGCGTGCTGAACTCGCTCCTCGTGATG
TAGTTGCCCGAGCTATCGATTTTGAAATGAAACGTTTAGGTGCAGATTGCATGTATTTAGACATTAGCCACAAGCCAGAA
GATTTCATTCATCATCATTTCCCTACCATTAATGAAAAATTGCTTTCGTTTGGTATTGATATGACAAAAGAACCCATTCC
TATCGTTCCAGCGGCACACTATACCTGTGGTGGCGTAATGGTTGATAAGCAGGGCCAAACAGACATTGATGGCTTGTTTG
CTATCGGTGAAGTAAGTTATACCGGACTTCATGGTGCCAATCGCCTAGCATCTAATTCATTATTAGAATGTGTTGTTTAT
GCTTGGTCTGCCGCTGAATGCATAATTAAAGAAATGGATAAGCGAGAGCTTCCGCCATCATTGCCAGCATGGGATGAAAG
CCAAGTTGAATGTTCAGATGAAGAAGTCGTGATTCAACATAACTGGCATGAATTACGCTTGTTTATGTGGGATTACATGG
GAATTGTACGTACCGACAAACGTTTAGAACGAGCGCTTCGTCGCATCCAATTGCTAAAGCAAGAAACGCATGATTATTAC
AGTAACTTCCGAGTCTCTAATAACCTCATTGAATTACGTAACTTACTCGATGTCTCTGAACTCATGATTCGTTGTGCTAT
GGAGCGAAAAGAAAGTTGTGGCTTACATTACACCCTTGATTATCCTGAAGCCTTAGAAGAATCAGGCCCAACGATTTTAA
TACCAACGCGTGATTAA

Upstream 100 bases:

>100_bases
CCTAATTTTATTCTTGATAATTAAGATTCCACTCAAAATGTGACGCAACCGGATAGATTCTGGTAAAATGCTCTGATAAT
CACAGGATAATGATGAATAT

Downstream 100 bases:

>100_bases
TATCTAAGTAAAACGATCAATAAAAGCCCTATATAGAATCTTATCTATGTAGGGCTTTTTAATTTTATCGCATTACTAAA
AAATAGATTTACCAGATAAT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTIESHVDDTLIAGAHICDK
DVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISHPRFHLTREGGHSHRRILHAADATGKAMQTSLQDNVVNHPNITIF
ERHNALDLITEDKIGGDIDKVVGAYIWNRDAEHVETVRAKFVVIATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANL
EFNQFHPTCLFHPDARNFLMSEALRGEGAYLRRPDGSRFMLDFDERAELAPRDVVARAIDFEMKRLGADCMYLDISHKPE
DFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVY
AWSAAECIIKEMDKRELPPSLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQLLKQETHDYY
SNFRVSNNLIELRNLLDVSELMIRCAMERKESCGLHYTLDYPEALEESGPTILIPTRD

Sequences:

>Translated_538_residues
MNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTIESHVDDTLIAGAHICDK
DVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISHPRFHLTREGGHSHRRILHAADATGKAMQTSLQDNVVNHPNITIF
ERHNALDLITEDKIGGDIDKVVGAYIWNRDAEHVETVRAKFVVIATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANL
EFNQFHPTCLFHPDARNFLMSEALRGEGAYLRRPDGSRFMLDFDERAELAPRDVVARAIDFEMKRLGADCMYLDISHKPE
DFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVY
AWSAAECIIKEMDKRELPPSLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQLLKQETHDYY
SNFRVSNNLIELRNLLDVSELMIRCAMERKESCGLHYTLDYPEALEESGPTILIPTRD
>Mature_538_residues
MNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTIESHVDDTLIAGAHICDK
DVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISHPRFHLTREGGHSHRRILHAADATGKAMQTSLQDNVVNHPNITIF
ERHNALDLITEDKIGGDIDKVVGAYIWNRDAEHVETVRAKFVVIATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANL
EFNQFHPTCLFHPDARNFLMSEALRGEGAYLRRPDGSRFMLDFDERAELAPRDVVARAIDFEMKRLGADCMYLDISHKPE
DFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVY
AWSAAECIIKEMDKRELPPSLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQLLKQETHDYY
SNFRVSNNLIELRNLLDVSELMIRCAMERKESCGLHYTLDYPEALEESGPTILIPTRD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=520, Percent_Identity=32.3076923076923, Blast_Score=195, Evalue=8e-50,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=72.7611940298508, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=532, Percent_Identity=32.5187969924812, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=31.1942959001783, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=29.5880149812734, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=535, Percent_Identity=31.0280373831776, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71986328, Length=301, Percent_Identity=27.5747508305648, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322701, Length=560, Percent_Identity=32.1428571428571, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6322416, Length=565, Percent_Identity=31.3274336283186, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17137288, Length=558, Percent_Identity=30.6451612903226, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655642, Length=558, Percent_Identity=30.6451612903226, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655647, Length=558, Percent_Identity=30.6451612903226, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24663005, Length=558, Percent_Identity=29.0322580645161, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60308; Mature: 60308

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEEECCC
DTIESHVDDTLIAGAHICDKDVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISHPRFH
CHHHHHCCCHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LTREGGHSHRRILHAADATGKAMQTSLQDNVVNHPNITIFERHNALDLITEDKIGGDIDK
EEECCCCCHHEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCHHH
VVGAYIWNRDAEHVETVRAKFVVIATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANL
HHHHHHCCCCHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEE
EFNQFHPTCLFHPDARNFLMSEALRGEGAYLRRPDGSRFMLDFDERAELAPRDVVARAID
ECCCCCCEEEECCCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHH
FEMKRLGADCMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVM
HHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCEE
VDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVYAWSAAECIIKEMDKRELPPS
ECCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQLLKQETHDYY
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLIELRNLLDVSELMIRCAMERKESCGLHYTLDYPEALEESGPTILIPTRD
HCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEEECCC
>Mature Secondary Structure
MNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEEECCC
DTIESHVDDTLIAGAHICDKDVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISHPRFH
CHHHHHCCCHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LTREGGHSHRRILHAADATGKAMQTSLQDNVVNHPNITIFERHNALDLITEDKIGGDIDK
EEECCCCCHHEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCHHH
VVGAYIWNRDAEHVETVRAKFVVIATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANL
HHHHHHCCCCHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEE
EFNQFHPTCLFHPDARNFLMSEALRGEGAYLRRPDGSRFMLDFDERAELAPRDVVARAID
ECCCCCCEEEECCCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHH
FEMKRLGADCMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVM
HHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCEE
VDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVYAWSAAECIIKEMDKRELPPS
ECCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQLLKQETHDYY
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLIELRNLLDVSELMIRCAMERKESCGLHYTLDYPEALEESGPTILIPTRD
HCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]