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The map label for this gene is dcuB [H]

Identifier: 209695918

GI number: 209695918

Start: 2679390

End: 2680733

Strand: Direct

Name: dcuB [H]

Synonym: VSAL_I2500

Alternate gene names: 209695918

Gene position: 2679390-2680733 (Clockwise)

Preceding gene: 209695917

Following gene: 209695920

Centisome position: 80.58

GC content: 42.93

Gene sequence:

>1344_bases
TTGCATTTATGCAGGTGGTTGTATTTTGAGTTTATCTTTCTATTACTAGTGCTCTATATTGGCTCTCGCTTTGGCGGGAT
TGGTTTAGGCGTCGTTTCTGGGATTGGTCTGGTTATTGAAGTGTTTATCTTCAAAATGCCCCCTACATCTCCTCCCGTTA
CCGTTATGCTTATTATCTTAGCGGTTGTTACTTGTGCATCGATACTAGAAGCCGCTGGGGGCCTCAAGTACATGCTGCAA
GTCGCAGAGCGGATCTTAAGAAAGAACCCGAAACGCGTTACTATCATCGCTCCTTTTGTTACCTATGCGATGACATTCTT
GCTAGGTACAGGTCACGCGGTATATTCGATTATGCCTATCATTGGTGATGTAGCATTAAAAAATGGTATTCGACCTGAAC
GCCCTATGGCGGCGGCTTCTGTTGCCTCTCAAATCGCAATTACCGCTTCCCCTCTTTCTGCTGCGGTTGTTTACTATTTA
GCGCAATTAGTCGATATCGATGCATCAATTACCTTATTGTCGATCTTAATGGTTACGGTTCCTTCAACGTTATTTGGTAC
ACTAATGTTGGCGCTGTACAGTTTACGTCGTGGTAAAGAATTAGACGATGATGAAGAGTATCAAGCTCGTTTAAAAGATC
CTATTTGGCGTGAAAAAATCTTAAACACTACCTCTACGTCTTTAGATGAAAAACTACCAACCAAAGCCCGCAATGCGGTA
CTTATCTTTTTAGCTGCCATCGTATCTATTGTTGTCATCGCTATGTTGCCAGAGATCCGTACCATCACAGAAGGTGGCGC
TCCGATCAAAATGTCAGTCGTGATCCAAATGATGATGTTGGCGTTTGGTGGTGTCATTCTATTAGCGACGAAGACCGATC
CTCGTGATGTTCCAAATGGTGTGGTGTTTAAATCAGGTATGGTTGCCGCGATTGCTATCTTCGGTATTGCTTGGATGTCA
GATACCTACTTCCAATACGCAATGCCTCAATTTAGAGCCGGAATTGTGGATATGGTGACACTTCACCCATGGACATTCAC
TTTAGCGCTATTCATTGTATCTGTAGTGGTTAACTCTCAAGCAGCAACGGCTCGAATGATGTTACCTGTTGGCCTTGCTC
TAGGTCTAGAGCCTGCTTTGGTGATTGGTTTAATGCCTGCAGTTTATGGTTACTTCTTTATTCCAAACTACCCATCAGAT
ATTGCTACGGTTAACTTTGATAGCTCAGGTACGACTAAGATTGGTAAATACTACTTTAACCACTCGTTTATGTCGGTTGG
TTTAATCGGGGTAATTTCGGCCTGTATGGTCGGTTATGTATTAGGACAAATAGTGATTGGTTAA

Upstream 100 bases:

>100_bases
CTCTATTTACAAAACCAACAACACTCTAAATGAGAACAAGCTCAATAAAAGCCTCTGATATATAAGGTATTAATGCCTGT
AATTGATTTTTTATTATTTT

Downstream 100 bases:

>100_bases
GGCAACCTAATCAGACTTAACGTTTAAAAAAAAGCCCGTGAGCATGGTTGTTAATAACAACCTGTATCACGGGCTTTTTT
AAAACAATCTTACAGAGATA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MHLCRWLYFEFIFLLLVLYIGSRFGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQ
VAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYL
AQLVDIDASITLLSILMVTVPSTLFGTLMLALYSLRRGKELDDDEEYQARLKDPIWREKILNTTSTSLDEKLPTKARNAV
LIFLAAIVSIVVIAMLPEIRTITEGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMS
DTYFQYAMPQFRAGIVDMVTLHPWTFTLALFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSD
IATVNFDSSGTTKIGKYYFNHSFMSVGLIGVISACMVGYVLGQIVIG

Sequences:

>Translated_447_residues
MHLCRWLYFEFIFLLLVLYIGSRFGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQ
VAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYL
AQLVDIDASITLLSILMVTVPSTLFGTLMLALYSLRRGKELDDDEEYQARLKDPIWREKILNTTSTSLDEKLPTKARNAV
LIFLAAIVSIVVIAMLPEIRTITEGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMS
DTYFQYAMPQFRAGIVDMVTLHPWTFTLALFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSD
IATVNFDSSGTTKIGKYYFNHSFMSVGLIGVISACMVGYVLGQIVIG
>Mature_447_residues
MHLCRWLYFEFIFLLLVLYIGSRFGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIILAVVTCASILEAAGGLKYMLQ
VAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPIIGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYL
AQLVDIDASITLLSILMVTVPSTLFGTLMLALYSLRRGKELDDDEEYQARLKDPIWREKILNTTSTSLDEKLPTKARNAV
LIFLAAIVSIVVIAMLPEIRTITEGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNGVVFKSGMVAAIAIFGIAWMS
DTYFQYAMPQFRAGIVDMVTLHPWTFTLALFIVSVVVNSQAATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSD
IATVNFDSSGTTKIGKYYFNHSFMSVGLIGVISACMVGYVLGQIVIG

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=403, Percent_Identity=47.1464019851117, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790580, Length=444, Percent_Identity=35.5855855855856, Blast_Score=251, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 48433; Mature: 48433

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLCRWLYFEFIFLLLVLYIGSRFGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIIL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AVVTCASILEAAGGLKYMLQVAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPI
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHH
IGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVDIDASITLLSILMVTV
HHHHHHHCCCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PSTLFGTLMLALYSLRRGKELDDDEEYQARLKDPIWREKILNTTSTSLDEKLPTKARNAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCHHHHHHH
LIFLAAIVSIVVIAMLPEIRTITEGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNG
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
VVFKSGMVAAIAIFGIAWMSDTYFQYAMPQFRAGIVDMVTLHPWTFTLALFIVSVVVNSQ
CEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCC
AATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVNFDSSGTTKIGKYYFN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHH
HSFMSVGLIGVISACMVGYVLGQIVIG
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHLCRWLYFEFIFLLLVLYIGSRFGGIGLGVVSGIGLVIEVFIFKMPPTSPPVTVMLIIL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AVVTCASILEAAGGLKYMLQVAERILRKNPKRVTIIAPFVTYAMTFLLGTGHAVYSIMPI
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHH
IGDVALKNGIRPERPMAAASVASQIAITASPLSAAVVYYLAQLVDIDASITLLSILMVTV
HHHHHHHCCCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PSTLFGTLMLALYSLRRGKELDDDEEYQARLKDPIWREKILNTTSTSLDEKLPTKARNAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHCCHHHHHHH
LIFLAAIVSIVVIAMLPEIRTITEGGAPIKMSVVIQMMMLAFGGVILLATKTDPRDVPNG
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
VVFKSGMVAAIAIFGIAWMSDTYFQYAMPQFRAGIVDMVTLHPWTFTLALFIVSVVVNSQ
CEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCC
AATARMMLPVGLALGLEPALVIGLMPAVYGYFFIPNYPSDIATVNFDSSGTTKIGKYYFN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHH
HSFMSVGLIGVISACMVGYVLGQIVIG
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]