Click here to switch to the map view.

The map label for this gene is purM

Identifier: 209695815

GI number: 209695815

Start: 2559268

End: 2560308

Strand: Direct

Name: purM

Synonym: VSAL_I2390

Alternate gene names: 209695815

Gene position: 2559268-2560308 (Clockwise)

Preceding gene: 209695813

Following gene: 209695816

Centisome position: 76.97

GC content: 42.17

Gene sequence:

>1041_bases
GTGAGCGACAACAAAACTTCTCTTAGCTACAAAGATGCTGGCGTAGACATTGATGCTGGTAATGCACTTGTTGATCGAAT
TAAAGGCGTAGTGAAACGTACTCGTCGCCCAGAAGTTATGGGTGGCATTGGTGGTTTTGGTGCGTTATGTGAACTTCCAA
CGAAATATAAACAACCTGTTTTAGTTTCTGGTACTGATGGTGTAGGAACTAAACTTCGTTTAGCTTTAGACCTAAACAAG
CACGATACGATTGGTATCGACTTAGTGGCTATGTGTGTAAATGACCTAATTGTTCAAGGTGGTGAACCTCTGTTCTTCCT
TGATTATTATGCAACAGGCAAACTCGATATTGATGTCGCAGCTCAAGTAGTGACGGGCATTGGTGAAGGTTGTATTCAAG
CTGGTTGTGCATTGATCGGTGGTGAAACGGCTGAAATGCCAGGCATGTATGAAGGCGAAGATTATGATGTTGCTGGATTC
TGTGTCGGTGTTGTCGAGAAAGAAGACATCATTAATGGCACTAAAGTCGCTGCAGGTGATGCATTAATCGCTGTAGGTTC
AAGTGGCCCTCACTCAAATGGTTATTCATTAATTCGTAAAATCCTTGAAGTATCAAATGCGGATCTTACAGAAGAATTAA
ATGGCAGAACGATTGCAGATCATTTAATCGAACCAACAAAAATCTACATTAAATCAGCACTTAAAATGATTGCAGAGCAT
GATATTCATGCTATCTCTCATATTACGGGTGGCGGTTTCTGGGAAAATATTCCACGAGTACTTCCTGAAGGAACTAAAGC
GGTTGTTGATGGTAAGAGTTGGGAGTGGCCTGCTATCTTTAACTGGCTACAAGAAAAAGGCAACGTAGATACCTACGAAA
TGTACCGTACCTTTAACTGTGGTGTTGGTCTTATTGTTGCCCTTCCAAAAGAACAAGCTGAACAAGCTGTCGCATTATTG
AATGCGGAAGGCGAAAACGCTTGGGTTATTGGCAAAGTAGCGGCAGCAGAGCAAGGCGAAGAACAAGTTGAGATCCGTTA
A

Upstream 100 bases:

>100_bases
AAATTTCTCCAAATTTCCTTACGCAAACGTTTCCCTTTTTACATTGTGCTGTTAGAATGGCGCGGATTTTTGAAATCCAA
CCTATGGCGAGGACACCACC

Downstream 100 bases:

>100_bases
TCGGGACTTACAAACTTTGAACTAGTTCATAAAGAATAAATAAAAGGTGATCAATTATTCGGTCACCTTTTTTATATCTG
TAATTTGTGTTCGAATGGTT

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSDNKTSLSYKDAGVDIDAGNALVDRIKGVVKRTRRPEVMGGIGGFGALCELPTKYKQPVLVSGTDGVGTKLRLALDLNK
HDTIGIDLVAMCVNDLIVQGGEPLFFLDYYATGKLDIDVAAQVVTGIGEGCIQAGCALIGGETAEMPGMYEGEDYDVAGF
CVGVVEKEDIINGTKVAAGDALIAVGSSGPHSNGYSLIRKILEVSNADLTEELNGRTIADHLIEPTKIYIKSALKMIAEH
DIHAISHITGGGFWENIPRVLPEGTKAVVDGKSWEWPAIFNWLQEKGNVDTYEMYRTFNCGVGLIVALPKEQAEQAVALL
NAEGENAWVIGKVAAAEQGEEQVEIR

Sequences:

>Translated_346_residues
MSDNKTSLSYKDAGVDIDAGNALVDRIKGVVKRTRRPEVMGGIGGFGALCELPTKYKQPVLVSGTDGVGTKLRLALDLNK
HDTIGIDLVAMCVNDLIVQGGEPLFFLDYYATGKLDIDVAAQVVTGIGEGCIQAGCALIGGETAEMPGMYEGEDYDVAGF
CVGVVEKEDIINGTKVAAGDALIAVGSSGPHSNGYSLIRKILEVSNADLTEELNGRTIADHLIEPTKIYIKSALKMIAEH
DIHAISHITGGGFWENIPRVLPEGTKAVVDGKSWEWPAIFNWLQEKGNVDTYEMYRTFNCGVGLIVALPKEQAEQAVALL
NAEGENAWVIGKVAAAEQGEEQVEIR
>Mature_345_residues
SDNKTSLSYKDAGVDIDAGNALVDRIKGVVKRTRRPEVMGGIGGFGALCELPTKYKQPVLVSGTDGVGTKLRLALDLNKH
DTIGIDLVAMCVNDLIVQGGEPLFFLDYYATGKLDIDVAAQVVTGIGEGCIQAGCALIGGETAEMPGMYEGEDYDVAGFC
VGVVEKEDIINGTKVAAGDALIAVGSSGPHSNGYSLIRKILEVSNADLTEELNGRTIADHLIEPTKIYIKSALKMIAEHD
IHAISHITGGGFWENIPRVLPEGTKAVVDGKSWEWPAIFNWLQEKGNVDTYEMYRTFNCGVGLIVALPKEQAEQAVALLN
AEGENAWVIGKVAAAEQGEEQVEIR

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=346, Percent_Identity=48.5549132947977, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI209869995, Length=346, Percent_Identity=48.5549132947977, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI209869993, Length=346, Percent_Identity=48.5549132947977, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1788845, Length=343, Percent_Identity=73.469387755102, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17567511, Length=343, Percent_Identity=41.6909620991254, Blast_Score=257, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6321203, Length=338, Percent_Identity=47.3372781065089, Blast_Score=311, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24582400, Length=292, Percent_Identity=40.0684931506849, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_ALISL (B6EJU3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263745.1
- GeneID:   6989358
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2390
- HOGENOM:   HBG531222
- OMA:   GIDMIAM
- ProtClustDB:   PRK05385
- GO:   GO:0005737
- HAMAP:   MF_00741_B
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 36979; Mature: 36847

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKTSLSYKDAGVDIDAGNALVDRIKGVVKRTRRPEVMGGIGGFGALCELPTKYKQPV
CCCCCCCCEECCCCCEECCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHCCCHHHCCCE
LVSGTDGVGTKLRLALDLNKHDTIGIDLVAMCVNDLIVQGGEPLFFLDYYATGKLDIDVA
EEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEHHHH
AQVVTGIGEGCIQAGCALIGGETAEMPGMYEGEDYDVAGFCVGVVEKEDIINGTKVAAGD
HHHHHHCCHHHHHHCHHEECCCCCCCCCCCCCCCCCEEEEEEEEECHHHHCCCCEEECCC
ALIAVGSSGPHSNGYSLIRKILEVSNADLTEELNGRTIADHLIEPTKIYIKSALKMIAEH
EEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
DIHAISHITGGGFWENIPRVLPEGTKAVVDGKSWEWPAIFNWLQEKGNVDTYEMYRTFNC
HHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHEEEECC
GVGLIVALPKEQAEQAVALLNAEGENAWVIGKVAAAEQGEEQVEIR
CCEEEEECCHHHHHHHHHHEECCCCCEEEEEEEHHCCCCCCCCCCC
>Mature Secondary Structure 
SDNKTSLSYKDAGVDIDAGNALVDRIKGVVKRTRRPEVMGGIGGFGALCELPTKYKQPV
CCCCCCCEECCCCCEECCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHCCCHHHCCCE
LVSGTDGVGTKLRLALDLNKHDTIGIDLVAMCVNDLIVQGGEPLFFLDYYATGKLDIDVA
EEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEHHHH
AQVVTGIGEGCIQAGCALIGGETAEMPGMYEGEDYDVAGFCVGVVEKEDIINGTKVAAGD
HHHHHHCCHHHHHHCHHEECCCCCCCCCCCCCCCCCEEEEEEEEECHHHHCCCCEEECCC
ALIAVGSSGPHSNGYSLIRKILEVSNADLTEELNGRTIADHLIEPTKIYIKSALKMIAEH
EEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
DIHAISHITGGGFWENIPRVLPEGTKAVVDGKSWEWPAIFNWLQEKGNVDTYEMYRTFNC
HHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHEEEECC
GVGLIVALPKEQAEQAVALLNAEGENAWVIGKVAAAEQGEEQVEIR
CCEEEEECCHHHHHHHHHHEECCCCCEEEEEEEHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA