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The map label for this gene is pyrP [H]

Identifier: 209695813

GI number: 209695813

Start: 2557012

End: 2558262

Strand: Direct

Name: pyrP [H]

Synonym: VSAL_I2388

Alternate gene names: 209695813

Gene position: 2557012-2558262 (Clockwise)

Preceding gene: 209695811

Following gene: 209695815

Centisome position: 76.9

GC content: 42.45

Gene sequence:

>1251_bases
ATGCTATACAATTTATTCCAAGGTTTTCAGATGCTTTTTGTTGCATTTGGTGCCCTTGTTCTTGTTCCTCTTCTTACTGG
GCTCGATCCTAACGTCGCTCTTTTTGGTGCCGGTGTTGGTACTCTTCTTTTTCAACTTGTTACTCGTCGTTCAGTGCCTA
TCTTCCTCGCGTCTTCTTTTGCTTTTATTGCCCCTATTATTTATGGAACCCAAACATGGGGGATCCCAGCAACAATGGGT
GGCCTAATGGTTGCCGGTTTAGTTTATGTTGCGTTAGGTGCCGTTATTAAAGTGAAAGGGGTCGCTTTCATTCATAAGTT
ACTGCCTCCTGTTGTTGTTGGCCCTGTAATTATGGTGATCGGCCTAGGCTTAGCGCCAACAGCGGTTAATATGGCGTTAG
GAAAAACGGGCGATGGTGGATTTCAATTAGTTGATGGCCAATTGGCTTTCTGGATTGCCGCTGCATCATTATTTACCACA
ATTGGGGTGAGTGTTTTTTCAAAAGGGTTCTTTAGATTATTACCTATTCTCTCAGGCATCATTGTTGGCTACTCATTAAG
TTTAATTTTCGGCATCGTTGATTTTACGCCAGTACAACAAGCGGCATGGTTTGCACTACCCAACTTCACTTATCCTGAAT
TTAACATTAATGCCATTCTATTTATGATCCCAGTCGCCATCGCCCCTGCGGTTGAGCACGTGGGTGATATGTTGGCAATT
TCAAATGTAACCGGAAAAGACTACCTTAAAAAACCAGGCTTGCACCGCACCATTACTGGTGATGGTATTGCAACCATGGC
AGCCTCTCTTATTGGCGCGCCACCAAATACAACTTACAGTGAAGTGACTGGTGCGGTAATGCTGACTAAAGCGTTTAATC
CAGTGATCATGACATGGGCTGCCGTCTGTGCGATTGTTTTGGCTCTTGTGGGTAAATTAGGCGCAATATTACAAACTATT
CCAGTCCCTGTAATGGGGGGCATTATGATCTTACTGTTTGGTTCAATCGCGACGATTGGTCTAAATACTCTGATTAAAAA
CCAAGTTGATTTACATAAAGCACGTAACTTAACCATTGTTGGCGTTACCTTGGTATTTGGTATTGGTGGTATGGCTGTGG
GTATTGGCGATTTTAACTTACAGGGCGTAAGTCTTTGTGGGATTGTAGCTATTGCTCTTAATTTAATTCTTCCTGATGAC
TTAGGTGAAAACCATGTTGTTGATAATACACAAATGGAAGATGATAAATAA

Upstream 100 bases:

>100_bases
TGATACTTTGTGTAAAAGTTTTATTTTTTCACGATTAAAATCAAGATATTTATTATTATAAAAGTGATAGAATCCCTCAA
ATTCACTACTGGAAATAGTA

Downstream 100 bases:

>100_bases
ACCTAAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGTCTAGGGTAAATAAAAAG
GATTGATGGTTACCCATCAA

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLYNLFQGFQMLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMG
GLMVAGLVYVALGAVIKVKGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGGFQLVDGQLAFWIAAASLFTT
IGVSVFSKGFFRLLPILSGIIVGYSLSLIFGIVDFTPVQQAAWFALPNFTYPEFNINAILFMIPVAIAPAVEHVGDMLAI
SNVTGKDYLKKPGLHRTITGDGIATMAASLIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAILQTI
PVPVMGGIMILLFGSIATIGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAIALNLILPDD
LGENHVVDNTQMEDDK

Sequences:

>Translated_416_residues
MLYNLFQGFQMLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMG
GLMVAGLVYVALGAVIKVKGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGGFQLVDGQLAFWIAAASLFTT
IGVSVFSKGFFRLLPILSGIIVGYSLSLIFGIVDFTPVQQAAWFALPNFTYPEFNINAILFMIPVAIAPAVEHVGDMLAI
SNVTGKDYLKKPGLHRTITGDGIATMAASLIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAILQTI
PVPVMGGIMILLFGSIATIGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAIALNLILPDD
LGENHVVDNTQMEDDK
>Mature_416_residues
MLYNLFQGFQMLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMG
GLMVAGLVYVALGAVIKVKGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGGFQLVDGQLAFWIAAASLFTT
IGVSVFSKGFFRLLPILSGIIVGYSLSLIFGIVDFTPVQQAAWFALPNFTYPEFNINAILFMIPVAIAPAVEHVGDMLAI
SNVTGKDYLKKPGLHRTITGDGIATMAASLIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAILQTI
PVPVMGGIMILLFGSIATIGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAIALNLILPDD
LGENHVVDNTQMEDDK

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=432, Percent_Identity=24.0740740740741, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI44680143, Length=435, Percent_Identity=24.367816091954, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI44680148, Length=430, Percent_Identity=22.7906976744186, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI40316845, Length=430, Percent_Identity=22.7906976744186, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788843, Length=412, Percent_Identity=39.3203883495146, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI87081818, Length=410, Percent_Identity=38.2926829268293, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082181, Length=381, Percent_Identity=25.9842519685039, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082178, Length=334, Percent_Identity=26.3473053892216, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790087, Length=377, Percent_Identity=25.1989389920424, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=177, Percent_Identity=28.2485875706215, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=426, Percent_Identity=21.830985915493, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17542260, Length=229, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356175, Length=428, Percent_Identity=25.9345794392523, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 43511; Mature: 43511

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]