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The map label for this gene is dapE

Identifier: 209695800

GI number: 209695800

Start: 2546317

End: 2547450

Strand: Direct

Name: dapE

Synonym: VSAL_I2375

Alternate gene names: 209695800

Gene position: 2546317-2547450 (Clockwise)

Preceding gene: 209695799

Following gene: 209695801

Centisome position: 76.58

GC content: 43.21

Gene sequence:

>1134_bases
ATGCCTGACACTCCAACACTCACTCTCGCGAAAGATCTTCTCAGTCGCCAATCAGTCACTCCAGAAGATGCGGGCTGCCA
AGAGTTAATGATTAAACGCCTTAAGGCTCTTGGTTTTACAATTGAAATCATGGTATTTGAAGACACCACCAATTTTTGGG
CTCGCCGTGGAACAGAGGCACCTTTATTTACCTTCGCAGGCCACACAGATGTCGTTCCAACAGGATCATTAACGCAGTGG
AATACCGATCCTTTTGAGCCAACGATCATCGATGGCATGTTATACGCTCGTGGAGCTGCCGATATGAAAGGCTCTCTTGC
CTGCATGATTGTTGCTGTTGAGCGTTTCATTTCTGAGCACCCAGAACATAAAGGTTCACTCTCTTTCTTAATCACTTCAG
ATGAAGAAGGTCCATTCATTAATGGCACAACACGTGTGGTTGATACGCTAAAAGAGCGTAATGAAATTATTGATATGTGT
ATTGTTGGTGAACCGTCAAGCACCCAATATGTTGGCGATGTAGTCAAGAACGGTCGTAGAGGCTCTTTAACTGGAAACTT
AACCGTCAAAGGCATTCAAGGTCACGTTGCTTATCCGCACATTGCACGAAATCCGATCCACCAATCAATGGCGGCACTAC
TTGAACTGACAATGACTGAATGGGATTTAGGTAATGCTTATTTTCCACCAACCAGCTTTCAAATTCCGAACATGAACAGT
GGTACAGGTGCTTCTAATGTGATCCCTGGAACAGCGGAAATCATGTTTAACTTTCGCTTCAGCACTGAATCTACCGTCGA
AGGCCTACAACAGCGTGTGATTGAACTCTTAGATAAACACAATTTAGAATACGATCTGGATTGGATTATCAATGGACTTC
CGTTCTTAACCGATACTGGCGATCTATTAACAGCCGTTGTTGATGCCGTTGCCACTGTTAATCAACAGAAGCCTGAATTA
CTAACAACAGGTGGAACATCCGACGGCCGCTTCATTGCTCAAATGGGTTCACAAGTAATTGAGCTTGGTCCGGTTAATGC
CACTATTCATAAAGTGAATGAGTGTGTAAAGGTTGATGATTTAGAAAAACTGACTGATATGTATCAAGAAGTCCTAAATA
ATTTACTCGCTTAG

Upstream 100 bases:

>100_bases
TAATCCATAACGATCTCTACCATTTAGGCTTCAAACCAGCTCAATATGATGCAATTTTTAACGGTTAATTTTATTTTAAA
GATAAGTAAAGGAATACAAC

Downstream 100 bases:

>100_bases
CCACCTTTTAAGTATATGATGAGAGGGTCTTGATCCTCTCGTTTTTTATTAGGTACCTTATGACATATACACAGCTCACC
GGTCTATCAACTCAACACCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MPDTPTLTLAKDLLSRQSVTPEDAGCQELMIKRLKALGFTIEIMVFEDTTNFWARRGTEAPLFTFAGHTDVVPTGSLTQW
NTDPFEPTIIDGMLYARGAADMKGSLACMIVAVERFISEHPEHKGSLSFLITSDEEGPFINGTTRVVDTLKERNEIIDMC
IVGEPSSTQYVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQSMAALLELTMTEWDLGNAYFPPTSFQIPNMNS
GTGASNVIPGTAEIMFNFRFSTESTVEGLQQRVIELLDKHNLEYDLDWIINGLPFLTDTGDLLTAVVDAVATVNQQKPEL
LTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVKVDDLEKLTDMYQEVLNNLLA

Sequences:

>Translated_377_residues
MPDTPTLTLAKDLLSRQSVTPEDAGCQELMIKRLKALGFTIEIMVFEDTTNFWARRGTEAPLFTFAGHTDVVPTGSLTQW
NTDPFEPTIIDGMLYARGAADMKGSLACMIVAVERFISEHPEHKGSLSFLITSDEEGPFINGTTRVVDTLKERNEIIDMC
IVGEPSSTQYVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQSMAALLELTMTEWDLGNAYFPPTSFQIPNMNS
GTGASNVIPGTAEIMFNFRFSTESTVEGLQQRVIELLDKHNLEYDLDWIINGLPFLTDTGDLLTAVVDAVATVNQQKPEL
LTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVKVDDLEKLTDMYQEVLNNLLA
>Mature_376_residues
PDTPTLTLAKDLLSRQSVTPEDAGCQELMIKRLKALGFTIEIMVFEDTTNFWARRGTEAPLFTFAGHTDVVPTGSLTQWN
TDPFEPTIIDGMLYARGAADMKGSLACMIVAVERFISEHPEHKGSLSFLITSDEEGPFINGTTRVVDTLKERNEIIDMCI
VGEPSSTQYVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQSMAALLELTMTEWDLGNAYFPPTSFQIPNMNSG
TGASNVIPGTAEIMFNFRFSTESTVEGLQQRVIELLDKHNLEYDLDWIINGLPFLTDTGDLLTAVVDAVATVNQQKPELL
TTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVKVDDLEKLTDMYQEVLNNLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=60.5898123324397, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1790395, Length=264, Percent_Identity=28.7878787878788, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ALISL (B6EJS8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263730.1
- ProteinModelPortal:   B6EJS8
- MEROPS:   M20.010
- GeneID:   6987724
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2375
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41369; Mature: 41237

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTPTLTLAKDLLSRQSVTPEDAGCQELMIKRLKALGFTIEIMVFEDTTNFWARRGTEA
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCC
PLFTFAGHTDVVPTGSLTQWNTDPFEPTIIDGMLYARGAADMKGSLACMIVAVERFISEH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
PEHKGSLSFLITSDEEGPFINGTTRVVDTLKERNEIIDMCIVGEPSSTQYVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCC
GSLTGNLTVKGIQGHVAYPHIARNPIHQSMAALLELTMTEWDLGNAYFPPTSFQIPNMNS
CCEEECEEEEECCCCEECCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCC
GTGASNVIPGTAEIMFNFRFSTESTVEGLQQRVIELLDKHNLEYDLDWIINGLPFLTDTG
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCH
DLLTAVVDAVATVNQQKPELLTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVKVDD
HHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCHHEECCCCCHHHHHHHHHHCHHH
LEKLTDMYQEVLNNLLA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDTPTLTLAKDLLSRQSVTPEDAGCQELMIKRLKALGFTIEIMVFEDTTNFWARRGTEA
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCC
PLFTFAGHTDVVPTGSLTQWNTDPFEPTIIDGMLYARGAADMKGSLACMIVAVERFISEH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
PEHKGSLSFLITSDEEGPFINGTTRVVDTLKERNEIIDMCIVGEPSSTQYVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCC
GSLTGNLTVKGIQGHVAYPHIARNPIHQSMAALLELTMTEWDLGNAYFPPTSFQIPNMNS
CCEEECEEEEECCCCEECCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCC
GTGASNVIPGTAEIMFNFRFSTESTVEGLQQRVIELLDKHNLEYDLDWIINGLPFLTDTG
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCH
DLLTAVVDAVATVNQQKPELLTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVKVDD
HHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHCCHHEECCCCCHHHHHHHHHHCHHH
LEKLTDMYQEVLNNLLA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA