Click here to switch to the map view.

The map label for this gene is dsbE [H]

Identifier: 209695707

GI number: 209695707

Start: 2450284

End: 2450832

Strand: Reverse

Name: dsbE [H]

Synonym: VSAL_I2275

Alternate gene names: 209695707

Gene position: 2450832-2450284 (Counterclockwise)

Preceding gene: 209695708

Following gene: 209695706

Centisome position: 73.71

GC content: 39.16

Gene sequence:

>549_bases
ATGAAAAAGTTTTTATTTGTGCCACTCGCTTTGTTTTTACTTTTAGTTGCCGTGTTTGCTACCCAATTAACGCGTAACTC
AAATGGCGATGATCCAACAAAACTGGAATCAGTTTTAGTGGGTAAACCGGTACCTGAATTTCGCTTAGAAGATCTAGCGG
AAAAAGGTAAGCTTTATGATCAAAGTATCTTTAGAGGCGAACCTCTGCTTCTAAATGTATGGGCGAAATGGTGTCCAACA
TGCTATGCAGAGCATCAATACCTTAATACGCTGGCAACTGAAGGCGTAAAAATTATTGGTATGAACTATAAAGATGAGCG
AGCAGGAACGGTCAAATGGCTTAAAGAATTAGGTAATCCATACTTAATTACGTTGTTTGATGGTGATGGCATGCTAGGAA
TGGATTTAGGTGTTTATGGTGCGCCTGAGACGTTCTTAATCGATAGAAATGGCATCGTTCGCTATCGTCACGTTGGTGAT
GTAAATGATCGTAATTGGAATGAGAAACTAAAACCAATGTACGATCAACTAGTTGCGGAGTCTAAATAA

Upstream 100 bases:

>100_bases
TCTGGGCTGGTACTCTATTCATGGCATTAGGCGGTGTGTTAGCAATGTCTGATAAGCGTTACCGTTTCCGTAAAACATCG
AAAAAACAGGAGGCATAATA

Downstream 100 bases:

>100_bases
TGAAGATGATATTACGTAAATTGAGTCTTCGTCTTGCTGCTATTCTTGCGTTGAGCTTTTTTGCTGCTCAAGCAAGTTAT
GCCACTATCGATTTGTATAA

Product: thiol:disulfide interchange protein DsbE (cytochrome c biogenesis protein CcmG)

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKKFLFVPLALFLLLVAVFATQLTRNSNGDDPTKLESVLVGKPVPEFRLEDLAEKGKLYDQSIFRGEPLLLNVWAKWCPT
CYAEHQYLNTLATEGVKIIGMNYKDERAGTVKWLKELGNPYLITLFDGDGMLGMDLGVYGAPETFLIDRNGIVRYRHVGD
VNDRNWNEKLKPMYDQLVAESK

Sequences:

>Translated_182_residues
MKKFLFVPLALFLLLVAVFATQLTRNSNGDDPTKLESVLVGKPVPEFRLEDLAEKGKLYDQSIFRGEPLLLNVWAKWCPT
CYAEHQYLNTLATEGVKIIGMNYKDERAGTVKWLKELGNPYLITLFDGDGMLGMDLGVYGAPETFLIDRNGIVRYRHVGD
VNDRNWNEKLKPMYDQLVAESK
>Mature_182_residues
MKKFLFVPLALFLLLVAVFATQLTRNSNGDDPTKLESVLVGKPVPEFRLEDLAEKGKLYDQSIFRGEPLLLNVWAKWCPT
CYAEHQYLNTLATEGVKIIGMNYKDERAGTVKWLKELGNPYLITLFDGDGMLGMDLGVYGAPETFLIDRNGIVRYRHVGD
VNDRNWNEKLKPMYDQLVAESK

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=181, Percent_Identity=60.2209944751381, Blast_Score=250, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 20718; Mature: 20718

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLFVPLALFLLLVAVFATQLTRNSNGDDPTKLESVLVGKPVPEFRLEDLAEKGKLYD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHH
QSIFRGEPLLLNVWAKWCPTCYAEHQYLNTLATEGVKIIGMNYKDERAGTVKWLKELGNP
HHHHCCCCHHEEHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCC
YLITLFDGDGMLGMDLGVYGAPETFLIDRNGIVRYRHVGDVNDRNWNEKLKPMYDQLVAE
EEEEEECCCCEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHC
SK
CC
>Mature Secondary Structure
MKKFLFVPLALFLLLVAVFATQLTRNSNGDDPTKLESVLVGKPVPEFRLEDLAEKGKLYD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHH
QSIFRGEPLLLNVWAKWCPTCYAEHQYLNTLATEGVKIIGMNYKDERAGTVKWLKELGNP
HHHHCCCCHHEEHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCC
YLITLFDGDGMLGMDLGVYGAPETFLIDRNGIVRYRHVGDVNDRNWNEKLKPMYDQLVAE
EEEEEECCCCEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHC
SK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]