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The map label for this gene is aat

Identifier: 209695654

GI number: 209695654

Start: 2384277

End: 2384987

Strand: Reverse

Name: aat

Synonym: VSAL_I2217

Alternate gene names: 209695654

Gene position: 2384987-2384277 (Counterclockwise)

Preceding gene: 209695662

Following gene: 209695653

Centisome position: 71.73

GC content: 39.38

Gene sequence:

>711_bases
ATGACTATCTATTTACCCGAACTTGAACAAGAACATGACGCTTCATTTCCTGACATAGAAAGCGCATTACATGATCCTGA
TGGCTTACTTGCTATGGGCGGTGATTTAACCCCCAAACGCCTATTACTCGCTTACTCCCATGGGATTTTCCCTTGGTATA
GCAATGACGATCCTATTCTATGGTGGAGCCCAAGTATAAGAGGTGTATTTATTCCAGAGAATTACACTCCATCAAAAAGC
CTTAAGAAATTCTTTAAAAAGTCAGGTTACTCCATCACCATAAATCATGCGACGTCTGATGTTATTTCATTATGCGCATC
AACTCGGCCAGCAGAAGAAACATGGATAATGCCAGAAATGATCGAGGCTTATCAAAACCTAGCTAAGTTAGGGCATTGCC
ATTCTGTCGAGGTTTGGTTTAATAATGAATTAGTCGGAGGGCTTTATGGTCTCCAAATTGGGCAAGTATTTTGTGGTGAA
TCGATGTTCTCAATAAAAACCAATGCATCAAAAATTGCCTTATGGAAATTTTGTGAGCACTTTGTTGCTTTTGGAGGCAA
ACTCATTGACTGCCAAATGATGAATCCTCACTTAGAAAGTTTAGGCGCGATTCCAATGGAACGACCTATATTTAATGAAA
GGTTACAGGTATTTTCAGCTATCTCAGTGTTCGAAAACTGTTATCAACCTCAATCACTTAGCAATGGATAA

Upstream 100 bases:

>100_bases
TATTCTTCATACTTGCAGAGTAACGAAAACCAACGCGTTGCGCTAGAGGCAATGTCGTCTCTCTAAATTAAAAATAATGA
ATACGTATTTCTTTTTGCCT

Downstream 100 bases:

>100_bases
ATTATGACTCAATATTCACTGCAAGTAGGACTAACCCCTGAAGGTACATGCAGCTATTTACCTGATCAAAAAGATCGATT
AGGCGTAATTATGGAACCTG

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTIYLPELEQEHDASFPDIESALHDPDGLLAMGGDLTPKRLLLAYSHGIFPWYSNDDPILWWSPSIRGVFIPENYTPSKS
LKKFFKKSGYSITINHATSDVISLCASTRPAEETWIMPEMIEAYQNLAKLGHCHSVEVWFNNELVGGLYGLQIGQVFCGE
SMFSIKTNASKIALWKFCEHFVAFGGKLIDCQMMNPHLESLGAIPMERPIFNERLQVFSAISVFENCYQPQSLSNG

Sequences:

>Translated_236_residues
MTIYLPELEQEHDASFPDIESALHDPDGLLAMGGDLTPKRLLLAYSHGIFPWYSNDDPILWWSPSIRGVFIPENYTPSKS
LKKFFKKSGYSITINHATSDVISLCASTRPAEETWIMPEMIEAYQNLAKLGHCHSVEVWFNNELVGGLYGLQIGQVFCGE
SMFSIKTNASKIALWKFCEHFVAFGGKLIDCQMMNPHLESLGAIPMERPIFNERLQVFSAISVFENCYQPQSLSNG
>Mature_235_residues
TIYLPELEQEHDASFPDIESALHDPDGLLAMGGDLTPKRLLLAYSHGIFPWYSNDDPILWWSPSIRGVFIPENYTPSKSL
KKFFKKSGYSITINHATSDVISLCASTRPAEETWIMPEMIEAYQNLAKLGHCHSVEVWFNNELVGGLYGLQIGQVFCGES
MFSIKTNASKIALWKFCEHFVAFGGKLIDCQMMNPHLESLGAIPMERPIFNERLQVFSAISVFENCYQPQSLSNG

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=223, Percent_Identity=52.0179372197309, Blast_Score=234, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_ALISL (B6EIX7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263583.1
- GeneID:   6987661
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2217
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 26590; Mature: 26458

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYLPELEQEHDASFPDIESALHDPDGLLAMGGDLTPKRLLLAYSHGIFPWYSNDDPIL
CEEECCCCCHHCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCEE
WWSPSIRGVFIPENYTPSKSLKKFFKKSGYSITINHATSDVISLCASTRPAEETWIMPEM
EECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHCCCHHH
IEAYQNLAKLGHCHSVEVWFNNELVGGLYGLQIGQVFCGESMFSIKTNASKIALWKFCEH
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
FVAFGGKLIDCQMMNPHLESLGAIPMERPIFNERLQVFSAISVFENCYQPQSLSNG
HHHHCCEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIYLPELEQEHDASFPDIESALHDPDGLLAMGGDLTPKRLLLAYSHGIFPWYSNDDPIL
EEECCCCCHHCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCEE
WWSPSIRGVFIPENYTPSKSLKKFFKKSGYSITINHATSDVISLCASTRPAEETWIMPEM
EECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHCCCHHH
IEAYQNLAKLGHCHSVEVWFNNELVGGLYGLQIGQVFCGESMFSIKTNASKIALWKFCEH
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
FVAFGGKLIDCQMMNPHLESLGAIPMERPIFNERLQVFSAISVFENCYQPQSLSNG
HHHHCCEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA