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The map label for this gene is metG

Identifier: 209695662

GI number: 209695662

Start: 2391897

End: 2393957

Strand: Reverse

Name: metG

Synonym: VSAL_I2226

Alternate gene names: 209695662

Gene position: 2393957-2391897 (Counterclockwise)

Preceding gene: 209695666

Following gene: 209695654

Centisome position: 72.0

GC content: 40.08

Gene sequence:

>2061_bases
ATGGCTACCGATCCGAGAAAAATTCTGGTTACATGTGCCCTACCGTATGCAAACGGTTCAATCCACTTAGGCCACATGCT
TGAGCATATCCAAGCGGATATCTGGGTTCGTTACCAACGCTTACGTGGCAACGACATTAACTTTATTTGTGCAGATGACG
CTCACGGCACTCCAATCATGCTTAAAGCTCAACAGATGGGTATCACTCCAGAAGAAATGATTGCAGCAGTTAGCGAAGAA
CATCAAAAAGATTTCGCAGGCTTCGACATTAGCTTTGATAACTATCACAGCACACACAGTGATGAAAACCGTGAATTAGC
GTCTAGTATCTACCTAGAATTGAAAAAAAATGGCTTTATTACTAGCCGTACAATTTCTCAGTTATTTGATCCAGAAAAAG
AAATGTTCTTACCTGACCGTTTCGTAAAAGGTACGTGTCCAAAATGTAAATCTGAAGAACAATACGGGGATAACTGTGAT
AACTGTGGCGAAACCTACAGCCCAACGGATCTTATCAACCCTAAATCAGCCGTTTCTGGTGCGACTCCAGTAATGAAAGA
TTCTGAACATTTCTTCTTTGACTTACCTCAGTTTGAAAGTATGTTAAAAGAGTGGACTCGTTCTGGCTCTCTTCAAACTG
AAACAGCAAACAAAATGCAAGAATGGTTTGAATCTGGCCTACAACAATGGGACATCTCACGTGATGCACCTTATTTTGGC
TTTGAAATCCCAGGCGAAACCAATAAATTCTTCTATGTTTGGTTAGATGCACCGATTGGTTACATGGGTTCATTTAAAAA
TCTATGTGATAAACGTGATGATTTAGACTTTGATGAGTATTGGAAACTAAACAGCACAACAGAGCTTTACCACTTTATCG
GTAAAGATATTGTTTATTTCCATAGCCTATTTTGGCCTGCAATGCTTGATGGTGCTGGCTTCCGTAAACCAAACAATGTA
TTTGTTCATGGTTACGTAACGGTAAATGGCGCAAAAATGTCTAAGTCGAAAGGCACATTCATTAAAGCTGGCACTTACTT
AAATCACCTAGACCCAGAATGCCTACGTTACTACTACGCCGCTAAACTAAATAGCCGTATTGATGATATTGATTTAAACC
TTGAAGATTTCACTCAACGTGTAAACTCAGATGTCGTAAACAAGATTGTTAACTTAGCATCTCGTAATGCTGGTTTCATT
ACTAAACGTTTTGACGGCAAACTGTCTGATAATTTTGTGGCTCCTGAGCTCTACAACGAATTCGTTGCTGCTTCAGATCG
TATTGCTGAGCTATATGAAACTCGTGAATTTAGTCGTGCTATTCGTGAAATTACCGCGCTTGCTGATAAAGCAAACCAAT
ACATTGACGAAAAAGCACCTTGGGTTCTTGCGAAAGAAGAAGGCAAAGAACAAGAGCTACAAGAAGTTGCTTCTGTGGGT
ATTAACTTGTTCCGTGTGCTAATGGCTTACCTAAAACCAGTAATGCCTGAACTTGCAGCACGAACTGAAGCCTTCTTAAA
CGAAACGTTAACGTGGGAATGTATTGCACAACCTCTAACTTCTCATGAAATCACGAAGTTTAAAGCACTGTTCAACCGTA
TTGATCCTAAGAAAGTAGAAGCAATGGTTGAAGAATCAAAAGAAGATGCTGCAATTGAAATGGCTGCAAAAGAGAAAGCA
GAAGCGGAAAAAGAAAAAGCAAACCAAACAGAACTAGACAAAGAACCAATCGCGGATGAGATTGAATTCGATGCATTCGA
AGCGGTTGATATGCGTATTGCTCGTATTATCTCTTGTGAAGAAATTCCAAAGGCAAACAAACTACTTAAATTTCAGCTAG
ACATTGGCGGTGAAACTCGTCAAGTATTTTCAGGGATTAAATCTGCATACACACCTGAAGAATTAGTTGGTAAGCATACT
GTGATGGTTGCTAACCTAAAACCTCGTAAAATGAAGTTTGGTATGTCTGAAGGCATGATTCTGGCTGCTGGCCCTGGTGG
TAAAGATCTTTGGATCCTTGAACCACACGAAGGTGCTCAACCTGGTATGCGTGTAATGTAA

Upstream 100 bases:

>100_bases
ATCATTAATTGAACCCTTGATCCTCTATGCATTCTGGCTCGCATCAGGTACTATTTATCTCTTAAAATTTTTACCCATCA
CATAAAGCGAAAGAGAAGAT

Downstream 100 bases:

>100_bases
CTCTTCTCTTTTAATAGAGAGCTTACAAACAAAAATAGAGACCATAAGGTCTCTATTTTTTTATCTGCATAACAATTTAT
AAGACGGGAGCCAACGACAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDINFICADDAHGTPIMLKAQQMGITPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSDENRELASSIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEEQYGDNCD
NCGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQTETANKMQEWFESGLQQWDISRDAPYFG
FEIPGETNKFFYVWLDAPIGYMGSFKNLCDKRDDLDFDEYWKLNSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDIDLNLEDFTQRVNSDVVNKIVNLASRNAGFI
TKRFDGKLSDNFVAPELYNEFVAASDRIAELYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVASVG
INLFRVLMAYLKPVMPELAARTEAFLNETLTWECIAQPLTSHEITKFKALFNRIDPKKVEAMVEESKEDAAIEMAAKEKA
EAEKEKANQTELDKEPIADEIEFDAFEAVDMRIARIISCEEIPKANKLLKFQLDIGGETRQVFSGIKSAYTPEELVGKHT
VMVANLKPRKMKFGMSEGMILAAGPGGKDLWILEPHEGAQPGMRVM

Sequences:

>Translated_686_residues
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDINFICADDAHGTPIMLKAQQMGITPEEMIAAVSEE
HQKDFAGFDISFDNYHSTHSDENRELASSIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEEQYGDNCD
NCGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQTETANKMQEWFESGLQQWDISRDAPYFG
FEIPGETNKFFYVWLDAPIGYMGSFKNLCDKRDDLDFDEYWKLNSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNV
FVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDIDLNLEDFTQRVNSDVVNKIVNLASRNAGFI
TKRFDGKLSDNFVAPELYNEFVAASDRIAELYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVASVG
INLFRVLMAYLKPVMPELAARTEAFLNETLTWECIAQPLTSHEITKFKALFNRIDPKKVEAMVEESKEDAAIEMAAKEKA
EAEKEKANQTELDKEPIADEIEFDAFEAVDMRIARIISCEEIPKANKLLKFQLDIGGETRQVFSGIKSAYTPEELVGKHT
VMVANLKPRKMKFGMSEGMILAAGPGGKDLWILEPHEGAQPGMRVM
>Mature_685_residues
ATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDINFICADDAHGTPIMLKAQQMGITPEEMIAAVSEEH
QKDFAGFDISFDNYHSTHSDENRELASSIYLELKKNGFITSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEEQYGDNCDN
CGETYSPTDLINPKSAVSGATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQTETANKMQEWFESGLQQWDISRDAPYFGF
EIPGETNKFFYVWLDAPIGYMGSFKNLCDKRDDLDFDEYWKLNSTTELYHFIGKDIVYFHSLFWPAMLDGAGFRKPNNVF
VHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYAAKLNSRIDDIDLNLEDFTQRVNSDVVNKIVNLASRNAGFIT
KRFDGKLSDNFVAPELYNEFVAASDRIAELYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVASVGI
NLFRVLMAYLKPVMPELAARTEAFLNETLTWECIAQPLTSHEITKFKALFNRIDPKKVEAMVEESKEDAAIEMAAKEKAE
AEKEKANQTELDKEPIADEIEFDAFEAVDMRIARIISCEEIPKANKLLKFQLDIGGETRQVFSGIKSAYTPEELVGKHTV
MVANLKPRKMKFGMSEGMILAAGPGGKDLWILEPHEGAQPGMRVM

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=30.2158273381295, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI24308436, Length=530, Percent_Identity=26.7924528301887, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=69.4323144104803, Blast_Score=1030, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=596, Percent_Identity=30.0335570469799, Blast_Score=265, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=24.2718446601942, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=23.6585365853659, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=560, Percent_Identity=29.6428571428571, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=550, Percent_Identity=22.9090909090909, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655311, Length=598, Percent_Identity=29.7658862876254, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21358483, Length=514, Percent_Identity=25.0972762645914, Blast_Score=152, Evalue=9e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_ALISL (B6EIY5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263591.1
- GeneID:   6989051
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2226
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77985; Mature: 77854

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDINFICADDAHGTPIM
CCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
LKAQQMGITPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSDENRELASSIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEEQYGDNCDNCGETYSPTDLINPKSAVSG
HHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQTETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGETNKFFYVWLDAPIGYMGSFKNLCDKRDDLDFDEYWKLNSTTELYHFIGKDIVYF
EECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHEECCCHHHHHHHHHHHHHHH
HSLFWPAMLDGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEECCHHHCCCHHHHHHHHH
AKLNSRIDDIDLNLEDFTQRVNSDVVNKIVNLASRNAGFITKRFDGKLSDNFVAPELYNE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
FVAASDRIAELYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVASVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNETLTWECIAQPLTSHEITKFKALFNRIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
AMVEESKEDAAIEMAAKEKAEAEKEKANQTELDKEPIADEIEFDAFEAVDMRIARIISCE
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EIPKANKLLKFQLDIGGETRQVFSGIKSAYTPEELVGKHTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEEEEECCCCHHHCCCCCCCEE
LAAGPGGKDLWILEPHEGAQPGMRVM
EEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATDPRKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRLRGNDINFICADDAHGTPIM
CCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
LKAQQMGITPEEMIAAVSEEHQKDFAGFDISFDNYHSTHSDENRELASSIYLELKKNGFI
EEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TSRTISQLFDPEKEMFLPDRFVKGTCPKCKSEEQYGDNCDNCGETYSPTDLINPKSAVSG
HHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHCCC
ATPVMKDSEHFFFDLPQFESMLKEWTRSGSLQTETANKMQEWFESGLQQWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPGETNKFFYVWLDAPIGYMGSFKNLCDKRDDLDFDEYWKLNSTTELYHFIGKDIVYF
EECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHEECCCHHHHHHHHHHHHHHH
HSLFWPAMLDGAGFRKPNNVFVHGYVTVNGAKMSKSKGTFIKAGTYLNHLDPECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEECCHHHCCCHHHHHHHHH
AKLNSRIDDIDLNLEDFTQRVNSDVVNKIVNLASRNAGFITKRFDGKLSDNFVAPELYNE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
FVAASDRIAELYETREFSRAIREITALADKANQYIDEKAPWVLAKEEGKEQELQEVASVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
INLFRVLMAYLKPVMPELAARTEAFLNETLTWECIAQPLTSHEITKFKALFNRIDPKKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
AMVEESKEDAAIEMAAKEKAEAEKEKANQTELDKEPIADEIEFDAFEAVDMRIARIISCE
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EIPKANKLLKFQLDIGGETRQVFSGIKSAYTPEELVGKHTVMVANLKPRKMKFGMSEGMI
HCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEEEEECCCCHHHCCCCCCCEE
LAAGPGGKDLWILEPHEGAQPGMRVM
EEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA