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The map label for this gene is polB [H]

Identifier: 209695583

GI number: 209695583

Start: 2297501

End: 2299870

Strand: Reverse

Name: polB [H]

Synonym: VSAL_I2142

Alternate gene names: 209695583

Gene position: 2299870-2297501 (Counterclockwise)

Preceding gene: 209695584

Following gene: 209695582

Centisome position: 69.17

GC content: 37.68

Gene sequence:

>2370_bases
TTGAACTCTCCTCACTGCGGCTTTTTATTAACTCGTCAGAATAAAGACATCAGAGACCATTCCCTCGTCGATTTATGGGT
TAAATGTGATGACCATATAGCACATTTATTAATTGAAAATGAATTGGCTGTTATTTTCTTTTTGAAAGAACGACAAGCTG
AATATGAGTCAATATTAACGAACGCACATATTCATTATTCAATTAAATCGACCTCTTTACATACTTTTCAGCATCAGCCT
GTTTTTGGTTTGTATTTTTCGAGTACTCAGCAAAAACGAGTAGCAACAGCCCTATTTGAAGATCATCAACTGCCGACTTT
TGAAGGTGATATTCGTTTAGCCGATCGTTATTTAATGGAACGTTTTATTTGTGGAGGAATGGCTTTTGTTGGTACTCCGC
GACAAAGAAAAGGATACGTTGAATATCGAGATGTAAAAATTAAATCCGCAGAAATGACACCTGAATTCAGTGTGGTTTCA
TTAGATGTAGAGTGTTCAGAAAAAGGTATTTTATATTCTGTAGCGCTGCACTGTGAGCGTGATACTCGTATCATTATGGT
AGGGCCAGAAGAGACGAGTGACTTACCGATAGAATGGGTTGAGAATGAAAAAGCATTGCTTCTTGCTCTAGAGTCTTGGT
TTCAAACGTTTGACCCCGATATTATTATTGGATGGAATGTCATTAACTTTGATTTTAATTTACTAATTAAAAGAGCCAAA
TGGCACAACCTTGCCTTTCGGTTAGGTCGAGGGAATTCCTCTCTTTATTTTCGAGAATCAAATAAAAATAGGCAACAAGG
TTTTTTATCTTTTCCCGGTCGGGTGGTTTTGGATGGCATTGATGCGTTAAAAACGGCGACATATCATTTTTCGAGTTGGT
CTTTAGAGTCGGTATCTCAAGAATTATTAAATGAAGGGAAATCGATACATGATCCGAGTGATCGGATGGGTGAGATAAAC
CGTATGTACCGTGAAGATAAATGCGCATTAGCAAAATATAATCTTCAAGATTGTGTGCTAGTTACTCGTATTTTTGAACT
AACACATTTACTAGAGTTTGTTATTGAAAGAACGAAATTGACCGGTATCGAACTCGATCGTGTTGGTGGTTCGGTTGCCG
CTTTCACTAATTTATATTTACCTCGATTGCATAGAGCAGGGTACATTGCTCCGAATTTAGAAAGTGAGAATTGGATTGCA
AGCCCTGGTGGTTATGTCATGAGCTCTAAACCGGGTCTATATGAATCGGTATTGGTCCTTGATTTTAAGTCGCTTTATCC
TTCTATTATTAAAACCTTTCGTATTGACCCGTTGGGGCTAGTTGAAGGCTTAAAATTAGAATCGGGGATAGATGATGGGC
AAGCTATCGAAGGATTTAGAGGTGGTCGTTTTCATAGAACAAAACATTTTTTACCTAATTTGATCGAAACCTTATGGTCA
GCGAGAGATGTCGCGAAGAAAAATAATGAAAAAGCGTTTTCTCAAGCAATTAAAATCATAATGAATTCTTTTTATGGGGT
GTTAGGATCATCTGGATGCCGATTTTTTGACCATCGTTTAGCGTCGTCAATTACGATGCGTGGGCATGAAATAATGAAAA
CAACCAGAGAGCTTATCGAATCTCAAGGGTATGAAGTGATCTATGGCGATACCGATTCCACCTTTGTTACCTTGGGGGAA
TCTATGCCATCTGATGAAGCGGATAAAATAGGCAAACAGTTAGTTGATCATATTAATCAATGGTGGACCGCGCATTTAGA
GTCTGAATACGCAATAGACTCTGCTTTAGAAATTGAATATGAAACGCATTATCGTATCTTTTTAATGCCGACTATTCGCG
GTTCTGAAATGGGATCGAAAAAACGCTACGCAGGGTTGATTAGAAAAGGCGATAAAGAAGAGATCATTTTTAAAGGATTA
GAAACGGTGAGAACCGATTGGACCCCTCTCTCTCAAGAGTTTCAAAAGGTACTTTTTGATAAAGTATTTCATCAACAAGC
TGTGGAAGAGTACGTACGTGAATATGTTGATAAAACAAAATCGGGTGAATTTGATAATAAGTTAATTTATCGTAAGCGTT
TACGTAGACAGTTAGATGAATATCAAAAGAATGTTCCACCGCATGTGAAGGCGGCAAGAATAGCCGATACACAAAACCAA
AAATTAGGAAAACCACTCATGTATCAACAAGGGGGGTGGATTGAATACATAATAACGACAGCGGGTCCTGAACCCATTGA
ATATCGCCAAAATCCGATTGATTATGATCACTATGTTGAGAAACAATTAAAGCCAATTGCTGAGGGCATACTTCCTTTTG
TTAATTTAAGTTTTACTGAATTATCGGCTTCTCAATTAGGTCTATTCTAA

Upstream 100 bases:

>100_bases
GCGAAAAGTACCTAAGCGCTCAGTTCTTGTTTTATTAAAAGAATTTGGAGATTGGGTTGTGAGCGAATTTAATAACATAG
AAAGAAGAATAGGTTAACGA

Downstream 100 bases:

>100_bases
CGATTAGTTTTGTTATATATTGAGTAATACATCTAAAAAACTTAGTAAAGTCAATAGGCTTTTAATAGCAAAAATTTGCG
TCCTAACGCATAATCAATGC

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MNSPHCGFLLTRQNKDIRDHSLVDLWVKCDDHIAHLLIENELAVIFFLKERQAEYESILTNAHIHYSIKSTSLHTFQHQP
VFGLYFSSTQQKRVATALFEDHQLPTFEGDIRLADRYLMERFICGGMAFVGTPRQRKGYVEYRDVKIKSAEMTPEFSVVS
LDVECSEKGILYSVALHCERDTRIIMVGPEETSDLPIEWVENEKALLLALESWFQTFDPDIIIGWNVINFDFNLLIKRAK
WHNLAFRLGRGNSSLYFRESNKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVSQELLNEGKSIHDPSDRMGEIN
RMYREDKCALAKYNLQDCVLVTRIFELTHLLEFVIERTKLTGIELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWIA
SPGGYVMSSKPGLYESVLVLDFKSLYPSIIKTFRIDPLGLVEGLKLESGIDDGQAIEGFRGGRFHRTKHFLPNLIETLWS
ARDVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKTTRELIESQGYEVIYGDTDSTFVTLGE
SMPSDEADKIGKQLVDHINQWWTAHLESEYAIDSALEIEYETHYRIFLMPTIRGSEMGSKKRYAGLIRKGDKEEIIFKGL
ETVRTDWTPLSQEFQKVLFDKVFHQQAVEEYVREYVDKTKSGEFDNKLIYRKRLRRQLDEYQKNVPPHVKAARIADTQNQ
KLGKPLMYQQGGWIEYIITTAGPEPIEYRQNPIDYDHYVEKQLKPIAEGILPFVNLSFTELSASQLGLF

Sequences:

>Translated_789_residues
MNSPHCGFLLTRQNKDIRDHSLVDLWVKCDDHIAHLLIENELAVIFFLKERQAEYESILTNAHIHYSIKSTSLHTFQHQP
VFGLYFSSTQQKRVATALFEDHQLPTFEGDIRLADRYLMERFICGGMAFVGTPRQRKGYVEYRDVKIKSAEMTPEFSVVS
LDVECSEKGILYSVALHCERDTRIIMVGPEETSDLPIEWVENEKALLLALESWFQTFDPDIIIGWNVINFDFNLLIKRAK
WHNLAFRLGRGNSSLYFRESNKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVSQELLNEGKSIHDPSDRMGEIN
RMYREDKCALAKYNLQDCVLVTRIFELTHLLEFVIERTKLTGIELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWIA
SPGGYVMSSKPGLYESVLVLDFKSLYPSIIKTFRIDPLGLVEGLKLESGIDDGQAIEGFRGGRFHRTKHFLPNLIETLWS
ARDVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKTTRELIESQGYEVIYGDTDSTFVTLGE
SMPSDEADKIGKQLVDHINQWWTAHLESEYAIDSALEIEYETHYRIFLMPTIRGSEMGSKKRYAGLIRKGDKEEIIFKGL
ETVRTDWTPLSQEFQKVLFDKVFHQQAVEEYVREYVDKTKSGEFDNKLIYRKRLRRQLDEYQKNVPPHVKAARIADTQNQ
KLGKPLMYQQGGWIEYIITTAGPEPIEYRQNPIDYDHYVEKQLKPIAEGILPFVNLSFTELSASQLGLF
>Mature_789_residues
MNSPHCGFLLTRQNKDIRDHSLVDLWVKCDDHIAHLLIENELAVIFFLKERQAEYESILTNAHIHYSIKSTSLHTFQHQP
VFGLYFSSTQQKRVATALFEDHQLPTFEGDIRLADRYLMERFICGGMAFVGTPRQRKGYVEYRDVKIKSAEMTPEFSVVS
LDVECSEKGILYSVALHCERDTRIIMVGPEETSDLPIEWVENEKALLLALESWFQTFDPDIIIGWNVINFDFNLLIKRAK
WHNLAFRLGRGNSSLYFRESNKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVSQELLNEGKSIHDPSDRMGEIN
RMYREDKCALAKYNLQDCVLVTRIFELTHLLEFVIERTKLTGIELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWIA
SPGGYVMSSKPGLYESVLVLDFKSLYPSIIKTFRIDPLGLVEGLKLESGIDDGQAIEGFRGGRFHRTKHFLPNLIETLWS
ARDVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKTTRELIESQGYEVIYGDTDSTFVTLGE
SMPSDEADKIGKQLVDHINQWWTAHLESEYAIDSALEIEYETHYRIFLMPTIRGSEMGSKKRYAGLIRKGDKEEIIFKGL
ETVRTDWTPLSQEFQKVLFDKVFHQQAVEEYVREYVDKTKSGEFDNKLIYRKRLRRQLDEYQKNVPPHVKAARIADTQNQ
KLGKPLMYQQGGWIEYIITTAGPEPIEYRQNPIDYDHYVEKQLKPIAEGILPFVNLSFTELSASQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=629, Percent_Identity=27.9809220985692, Blast_Score=163, Evalue=7e-40,
Organism=Homo sapiens, GI106507301, Length=674, Percent_Identity=23.5905044510386, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1786246, Length=789, Percent_Identity=50.063371356147, Blast_Score=790, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=632, Percent_Identity=27.0569620253165, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32565317, Length=644, Percent_Identity=25.4658385093168, Blast_Score=134, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324227, Length=604, Percent_Identity=24.8344370860927, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320101, Length=635, Percent_Identity=26.1417322834646, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24664937, Length=628, Percent_Identity=26.1146496815287, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24648780, Length=602, Percent_Identity=24.4186046511628, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90807; Mature: 90807

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPHCGFLLTRQNKDIRDHSLVDLWVKCDDHIAHLLIENELAVIFFLKERQAEYESILT
CCCCCCCEEEEECCCCCCCCCEEEEEEECCHHHHHHHEECCEEEEEEEEHHHHHHHHHHH
NAHIHYSIKSTSLHTFQHQPVFGLYFSSTQQKRVATALFEDHQLPTFEGDIRLADRYLME
CCEEEEEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RFICGGMAFVGTPRQRKGYVEYRDVKIKSAEMTPEFSVVSLDVECSEKGILYSVALHCER
HHHHCCCCEECCCHHHCCCEEEEEEEEEECCCCCCEEEEEEEEEECCCCEEEEEEEEECC
DTRIIMVGPEETSDLPIEWVENEKALLLALESWFQTFDPDIIIGWNVINFDFNLLIKRAK
CCEEEEECCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCEEEEEEEEEECHHHEEEHHH
WHNLAFRLGRGNSSLYFRESNKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVSQ
HCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHEEEECCCCHHHHHH
ELLNEGKSIHDPSDRMGEINRMYREDKCALAKYNLQDCVLVTRIFELTHLLEFVIERTKL
HHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
TGIELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWIASPGGYVMSSKPGLYESVLVL
CCEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH
DFKSLYPSIIKTFRIDPLGLVEGLKLESGIDDGQAIEGFRGGRFHRTKHFLPNLIETLWS
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHH
ARDVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKTTRELIE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
SQGYEVIYGDTDSTFVTLGESMPSDEADKIGKQLVDHINQWWTAHLESEYAIDSALEIEY
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
ETHYRIFLMPTIRGSEMGSKKRYAGLIRKGDKEEIIFKGLETVRTDWTPLSQEFQKVLFD
ECEEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KVFHQQAVEEYVREYVDKTKSGEFDNKLIYRKRLRRQLDEYQKNVPPHVKAARIADTQNQ
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
KLGKPLMYQQGGWIEYIITTAGPEPIEYRQNPIDYDHYVEKQLKPIAEGILPFVNLSFTE
HCCCCCEEECCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
LSASQLGLF
CCHHHCCCC
>Mature Secondary Structure
MNSPHCGFLLTRQNKDIRDHSLVDLWVKCDDHIAHLLIENELAVIFFLKERQAEYESILT
CCCCCCCEEEEECCCCCCCCCEEEEEEECCHHHHHHHEECCEEEEEEEEHHHHHHHHHHH
NAHIHYSIKSTSLHTFQHQPVFGLYFSSTQQKRVATALFEDHQLPTFEGDIRLADRYLME
CCEEEEEEECCEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RFICGGMAFVGTPRQRKGYVEYRDVKIKSAEMTPEFSVVSLDVECSEKGILYSVALHCER
HHHHCCCCEECCCHHHCCCEEEEEEEEEECCCCCCEEEEEEEEEECCCCEEEEEEEEECC
DTRIIMVGPEETSDLPIEWVENEKALLLALESWFQTFDPDIIIGWNVINFDFNLLIKRAK
CCEEEEECCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCEEEEEEEEEECHHHEEEHHH
WHNLAFRLGRGNSSLYFRESNKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVSQ
HCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHEEEECCCCHHHHHH
ELLNEGKSIHDPSDRMGEINRMYREDKCALAKYNLQDCVLVTRIFELTHLLEFVIERTKL
HHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
TGIELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWIASPGGYVMSSKPGLYESVLVL
CCEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH
DFKSLYPSIIKTFRIDPLGLVEGLKLESGIDDGQAIEGFRGGRFHRTKHFLPNLIETLWS
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHH
ARDVAKKNNEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKTTRELIE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
SQGYEVIYGDTDSTFVTLGESMPSDEADKIGKQLVDHINQWWTAHLESEYAIDSALEIEY
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
ETHYRIFLMPTIRGSEMGSKKRYAGLIRKGDKEEIIFKGLETVRTDWTPLSQEFQKVLFD
ECEEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KVFHQQAVEEYVREYVDKTKSGEFDNKLIYRKRLRRQLDEYQKNVPPHVKAARIADTQNQ
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
KLGKPLMYQQGGWIEYIITTAGPEPIEYRQNPIDYDHYVEKQLKPIAEGILPFVNLSFTE
HCCCCCEEECCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
LSASQLGLF
CCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]