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The map label for this gene is kefA [H]

Identifier: 209695497

GI number: 209695497

Start: 2194501

End: 2195376

Strand: Reverse

Name: kefA [H]

Synonym: VSAL_I2049

Alternate gene names: 209695497

Gene position: 2195376-2194501 (Counterclockwise)

Preceding gene: 209695498

Following gene: 209695496

Centisome position: 66.02

GC content: 38.81

Gene sequence:

>876_bases
ATGCTTCGTTTATTTATTCTCGTACTTACATTAAGCTTTGGATCGAGTGCTTTTGCTGAAGATCCTCAATCTATCGGCAA
CCTGCATCAATTCGCGAGCCTTATTCGTTGGAGTGGTGTTGCTTTCTCATTCCTTATTGTTATCGCCGCTTGGTTATTTC
TTCGTTTTATCCAATCGATTGTGGATGGAATAAGTAACCAGTTTGCTCAACGACGTATGTTGATGCAAAAGCTGCAATCC
TTCTTCCAATTTTTTATCTATATGTCGACAGGTATGACGGTGTTCATGCTCAGTTTCCGTGTTGATGAAAGAGTGTTAGC
CTTAATCGGTGGTACTCTCGCCGTTTCTGTTGGTTTCGCATTAAAAGATCTCACGGCCTCTTTTATTGCAGGCTTAACCA
TTATGATTGATCGACCTTTTCAAGTGGGCGACCGTGTCACGTTCGAAGGACACTATGGTGATATTATTGCTATCGGATTA
CGCTCCGTACGCATGCAAACACTTAATGATGACATCATCACCATCCCAAACAATAAATTCTTAAATGAAGTCGCTATTAG
TGGTAATTATGGTGCTTTGGATATGCAAGTCGTCATCCCTTTCTATATCGGAATGGATCAAGACATTAATGCGGCCCGCG
ATATTATTCAAGAAGCAGCTTCATCAAGTCGTTATATCCACTTACCAAAGCCTGTTGTTGTACTAGTAAAACAAACCATT
ACTGAAAATTATTTGGGCATTCAACTAACTTGTAAGGCTTATGTGGTTGATATTAAGTTTGAGAAATTATTTGAAACCGA
TATTACATTACGCGTAATGCAAGAATTTAGAAAAACAGATATCTACCCGCCAACAATATCTATCCAAAACAAATAA

Upstream 100 bases:

>100_bases
GGGTACATTGAATGGAGTGATGATAAAGCTCGAATTTCAGATCATTGGTTCCGACACATTACTAACGTACTTCATCGTCA
ACATTTATTGGTGAAATAAT

Downstream 100 bases:

>100_bases
TCGTGGTCACTTTCTATTGGTAGTTTCTCTGTAATCTACCAATAGATATACGATTCATCCTACCGAACTCTTCCAATATA
ACACTCGCTGTACTGTACAT

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLRLFILVLTLSFGSSAFAEDPQSIGNLHQFASLIRWSGVAFSFLIVIAAWLFLRFIQSIVDGISNQFAQRRMLMQKLQS
FFQFFIYMSTGMTVFMLSFRVDERVLALIGGTLAVSVGFALKDLTASFIAGLTIMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAISGNYGALDMQVVIPFYIGMDQDINAARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLGIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKTDIYPPTISIQNK

Sequences:

>Translated_291_residues
MLRLFILVLTLSFGSSAFAEDPQSIGNLHQFASLIRWSGVAFSFLIVIAAWLFLRFIQSIVDGISNQFAQRRMLMQKLQS
FFQFFIYMSTGMTVFMLSFRVDERVLALIGGTLAVSVGFALKDLTASFIAGLTIMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAISGNYGALDMQVVIPFYIGMDQDINAARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLGIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKTDIYPPTISIQNK
>Mature_291_residues
MLRLFILVLTLSFGSSAFAEDPQSIGNLHQFASLIRWSGVAFSFLIVIAAWLFLRFIQSIVDGISNQFAQRRMLMQKLQS
FFQFFIYMSTGMTVFMLSFRVDERVLALIGGTLAVSVGFALKDLTASFIAGLTIMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAISGNYGALDMQVVIPFYIGMDQDINAARDIIQEAASSSRYIHLPKPVVVLVKQTI
TENYLGIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKTDIYPPTISIQNK

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=194, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32858; Mature: 32858

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLFILVLTLSFGSSAFAEDPQSIGNLHQFASLIRWSGVAFSFLIVIAAWLFLRFIQSI
CHHHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDGISNQFAQRRMLMQKLQSFFQFFIYMSTGMTVFMLSFRVDERVLALIGGTLAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTIMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCC
LNEVAISGNYGALDMQVVIPFYIGMDQDINAARDIIQEAASSSRYIHLPKPVVVLVKQTI
HHHEEECCCCCCEEHEEEEEHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TENYLGIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKTDIYPPTISIQNK
HCCCCEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure
MLRLFILVLTLSFGSSAFAEDPQSIGNLHQFASLIRWSGVAFSFLIVIAAWLFLRFIQSI
CHHHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDGISNQFAQRRMLMQKLQSFFQFFIYMSTGMTVFMLSFRVDERVLALIGGTLAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTIMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCC
LNEVAISGNYGALDMQVVIPFYIGMDQDINAARDIIQEAASSSRYIHLPKPVVVLVKQTI
HHHEEECCCCCCEEHEEEEEHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TENYLGIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKTDIYPPTISIQNK
HCCCCEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]