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The map label for this gene is aladh [H]

Identifier: 209695306

GI number: 209695306

Start: 1972198

End: 1973316

Strand: Reverse

Name: aladh [H]

Synonym: VSAL_I1839

Alternate gene names: 209695306

Gene position: 1973316-1972198 (Counterclockwise)

Preceding gene: 209695312

Following gene: 209695305

Centisome position: 59.34

GC content: 43.25

Gene sequence:

>1119_bases
ATGATCATTGGCGTACCTAAAGAAATAAAAAATCACGAATACCGTGTTGGAATGATCCCAGCGAGTGTTCGAGAACTCAT
ATCTCATGGCCATGACGTTTTTGTCGAAACAAAAGCCGGCTCTGGCATTGGTTTTACCGACGATGATTATATCGCGGTAG
GCGCATCCATTCTTCCTACTGCTGAAGGCATTTTTGCAAAAGCAGAGATGATCGTAAAAGTTAAAGAGCCTCAAGCTGTT
GAACGTGCGATGCTGCGTGAAGGACAAATATTATTCACGTATTTACATCTTGCACCAGATTTTCCACAAACAGAAGACCT
AATTAAGAGTAAAGCTGTCTGCATCGCGTATGAGACTGTAACAGATTATATGGGTCGTCTGCCACTATTAGCGCCAATGT
CTGAAGTTGCAGGTAGAATGTCGATTCAAGCCGGTGCTCAAACACTTGAAAAGTCCAATGGTGGCCGTGGTTTACTTTTA
GGTGGCGTACCTGGTGTTGAGCCTGCAAAAGTTGTTATCGTCGGTGGCGGTGTCGTTGGTGCGAATGCTGCAAGAATGGC
GGTAGGTATGCGTGCTGACGTCACTATTTTAGACCGAAACCTTGATACACTTCGCTCTCTTGATGAAGAATTCCAAGGTC
GAGCAAAAGTGGTTTATTCAACCGTTGATGCGATTGAAAAGCATGTGTTAGAAGCGGACCTTGTTATTGGTGCCGTTCTT
ATACCTGGCGCAGCAGCACCGAAACTGGTTACGGCAGATCACATAAAACGTATGAAGCCAGGGGCCGCTGTTGTTGATGT
CGCTATCGACCAAGGTGGTTGCTTTGAAACATCACATGCAACAACGCATGCAGATCCAACTTATATCGTAGATGACGTTG
TTCACTATTGTGTGGCAAACATGCAGGGGGCCGTTGCAAGAACATCTACCTTTGCACTAAACAATGCAACATTACCTTAC
ATTATTAAATTGGCAGATAAAGGATACAAATCAGCTCTTCTTGAAGACAAAGGATTTTTAGATGGCTTAAATGTAATCAA
TGGTAAAATCACATGTAAAGAAGTTGCTGAAAGCTTCAACTTAGATTATATAACCCCTGAAGCTGCACTTAATGCTTAA

Upstream 100 bases:

>100_bases
TTAGCATCAAATTTTGCTTTTTACAATCTATACTAGATACAAATTCGAAACAAAAACCCTACAACCAGTTAAAACAACCA
TAACTAAAAAGGAATACAGG

Downstream 100 bases:

>100_bases
AAATCATACTCAATATTAAAATCATTTTAGGGCACCCATCGCGGTGCCCTTTCTTTACTTTAATAAATTCCATACAATCA
CCATATATGATTAAGGATGG

Product: alanine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIIGVPKEIKNHEYRVGMIPASVRELISHGHDVFVETKAGSGIGFTDDDYIAVGASILPTAEGIFAKAEMIVKVKEPQAV
ERAMLREGQILFTYLHLAPDFPQTEDLIKSKAVCIAYETVTDYMGRLPLLAPMSEVAGRMSIQAGAQTLEKSNGGRGLLL
GGVPGVEPAKVVIVGGGVVGANAARMAVGMRADVTILDRNLDTLRSLDEEFQGRAKVVYSTVDAIEKHVLEADLVIGAVL
IPGAAAPKLVTADHIKRMKPGAAVVDVAIDQGGCFETSHATTHADPTYIVDDVVHYCVANMQGAVARTSTFALNNATLPY
IIKLADKGYKSALLEDKGFLDGLNVINGKITCKEVAESFNLDYITPEAALNA

Sequences:

>Translated_372_residues
MIIGVPKEIKNHEYRVGMIPASVRELISHGHDVFVETKAGSGIGFTDDDYIAVGASILPTAEGIFAKAEMIVKVKEPQAV
ERAMLREGQILFTYLHLAPDFPQTEDLIKSKAVCIAYETVTDYMGRLPLLAPMSEVAGRMSIQAGAQTLEKSNGGRGLLL
GGVPGVEPAKVVIVGGGVVGANAARMAVGMRADVTILDRNLDTLRSLDEEFQGRAKVVYSTVDAIEKHVLEADLVIGAVL
IPGAAAPKLVTADHIKRMKPGAAVVDVAIDQGGCFETSHATTHADPTYIVDDVVHYCVANMQGAVARTSTFALNNATLPY
IIKLADKGYKSALLEDKGFLDGLNVINGKITCKEVAESFNLDYITPEAALNA
>Mature_372_residues
MIIGVPKEIKNHEYRVGMIPASVRELISHGHDVFVETKAGSGIGFTDDDYIAVGASILPTAEGIFAKAEMIVKVKEPQAV
ERAMLREGQILFTYLHLAPDFPQTEDLIKSKAVCIAYETVTDYMGRLPLLAPMSEVAGRMSIQAGAQTLEKSNGGRGLLL
GGVPGVEPAKVVIVGGGVVGANAARMAVGMRADVTILDRNLDTLRSLDEEFQGRAKVVYSTVDAIEKHVLEADLVIGAVL
IPGAAAPKLVTADHIKRMKPGAAVVDVAIDQGGCFETSHATTHADPTYIVDDVVHYCVANMQGAVARTSTFALNNATLPY
IIKLADKGYKSALLEDKGFLDGLNVINGKITCKEVAESFNLDYITPEAALNA

Specific function: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=354, Percent_Identity=29.6610169491525, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI122939153, Length=354, Percent_Identity=29.6610169491525, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1787887, Length=299, Percent_Identity=30.1003344481605, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17550456, Length=355, Percent_Identity=29.0140845070423, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39586; Mature: 39586

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNHEYRVGMIPASVRELISHGHDVFVETKAGSGIGFTDDDYIAVGASILPT
CEECCCHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCC
AEGIFAKAEMIVKVKEPQAVERAMLREGQILFTYLHLAPDFPQTEDLIKSKAVCIAYETV
CCCHHEEEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCEEEEEHHHH
TDYMGRLPLLAPMSEVAGRMSIQAGAQTLEKSNGGRGLLLGGVPGVEPAKVVIVGGGVVG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
ANAARMAVGMRADVTILDRNLDTLRSLDEEFQGRAKVVYSTVDAIEKHVLEADLVIGAVL
CCHHHHEECCCCCEEEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHE
IPGAAAPKLVTADHIKRMKPGAAVVDVAIDQGGCFETSHATTHADPTYIVDDVVHYCVAN
ECCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
MQGAVARTSTFALNNATLPYIIKLADKGYKSALLEDKGFLDGLNVINGKITCKEVAESFN
CCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHCCEEEHHHHHHHCC
LDYITPEAALNA
CCEECCHHHCCC
>Mature Secondary Structure
MIIGVPKEIKNHEYRVGMIPASVRELISHGHDVFVETKAGSGIGFTDDDYIAVGASILPT
CEECCCHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCC
AEGIFAKAEMIVKVKEPQAVERAMLREGQILFTYLHLAPDFPQTEDLIKSKAVCIAYETV
CCCHHEEEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCEEEEEHHHH
TDYMGRLPLLAPMSEVAGRMSIQAGAQTLEKSNGGRGLLLGGVPGVEPAKVVIVGGGVVG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
ANAARMAVGMRADVTILDRNLDTLRSLDEEFQGRAKVVYSTVDAIEKHVLEADLVIGAVL
CCHHHHEECCCCCEEEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHE
IPGAAAPKLVTADHIKRMKPGAAVVDVAIDQGGCFETSHATTHADPTYIVDDVVHYCVAN
ECCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
MQGAVARTSTFALNNATLPYIIKLADKGYKSALLEDKGFLDGLNVINGKITCKEVAESFN
CCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHCCEEEHHHHHHHCC
LDYITPEAALNA
CCEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA