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The map label for this gene is slt [H]

Identifier: 209695100

GI number: 209695100

Start: 1704213

End: 1706168

Strand: Reverse

Name: slt [H]

Synonym: VSAL_I1597

Alternate gene names: 209695100

Gene position: 1706168-1704213 (Counterclockwise)

Preceding gene: 209695101

Following gene: 209695099

Centisome position: 51.31

GC content: 38.6

Gene sequence:

>1956_bases
ATGTGTGCATTTATGAAAAAGGGATTTTCAATGCGGTCGAGTAAAGTTCATTGGTTATTAGCTTTTGTAAGTATATGCAA
CGTTTCTTATGCGTTCTCTGCCTCTGAATTGCGGTTAAATCGTTACGCCTATGATGCGATACAACAAGGGTTTAATCAGA
AGAACATCTCTATTTATGATAAGAAACGCGCCAGTATTGATAGCTATTCATTAGCCGCTTATACCGATTATCGTGCTTTC
TTGTTTGATCTCCCTAATAAAACCCCTGAGCAAGTGAATCAATTCTCGAAAGACTATGCGGATTACCCTTTTTCGGTGTC
TATACGTGCGGATTATCTTAATGCAATGATCGCGTCTAAGAACTGGGAAGCCTTGCTCCGTTATCAAACCACGCCACCTA
GAGATCAAGATTATCAATGTTACTATTACACCGCATTTTATCATCAAGGAAAACAAGAGGTTGCTTTTGAAGGCGCGAGA
TCTTTATGGCTTAATGGCGAAAGTGTTTCGAATGCATGTAATGATCTTTTTAGTTATTGGGAAAAAGCAGGGTTAAGAAC
GGATGATGTGATTAAGCGACGGGCTTTACTGTCGATAGAAGAGAGAAACCCAAGATTAATTACTTATCTAATTAAATTGG
TATCGAATAAACAAAGTCGTGTGGATTTAAATGAAATGTATGGCTTATATCAAACACCAACAAAGATAACGACAGTATTT
GATAAGAAAGTTGAGACGCCTTTTAATCGTGAATACGCACAAGTGATGCTAAGACATTTAGCAAGGAAAGACACCGATAA
AGCGTATTTAGCTTTCCGATTTATTAATGACCATTACTCATATACGAAAGACGAACTCCAAACTACCTCTGAATTTCTTG
CCGTTAGGCTTTTTCAGACGACGGATAGTGAAACCATCGAATGGCGAGATGATGTGTTTAAGCGTTCAAATCGTGATGAG
GTACTTGAACAACGAATTCGTTTATCGATACGTAAAACGCAATGGGAGAATATTAACTTTTGGATTTTGCGTTTGTCGGA
TAAAGCACAATCAACATATCGTTGGCAGTACTGGATGGCTCGAAGTGAAATCGCATTAGAGAAAGTAAATGAGGGTGAAG
AGCGATTAAAACGAATCTTGGGATATCGGCATATATACAGCTTTGCAGCAGCCATTGCGTTAAATCGAGAGATAACGTAT
CCAATTGCGAGTGTTGATACTAAGTTCGAAAAAATAGCACAATACCATCCTGATCTTGAAAGGGTTAAGGAGTTGCTTGC
CTTAGGTAAAGATGCCGCCGCTAAAAGTCAGTGGCGATATTTAATTGCAAGAGCCAGTTATGAGGATAGAGCTCGACTTG
CGACTTATGCGAGAAATGAAGGATGGGATGAATTTTCACTTGAAAGTACATTTAGAGGAGGGCTATGGGATCACATTGAA
TTGCGTTTCCCTGTTCGTTATTACTCATTGTTTACGCGTTATGGGGGTAAGTACAAGGTAAATCCAATTACGTTAATGTC
ACTGACAAGGCAAGAGAGCGGGTTTAACCCTAATGCTCACTCTCCAGTTGGTGCGCTAGGATTAATGCAAGTATTACCAA
GTACCGCTAGTTATATGGCGACGCTTTATACGTTGCCTTACAAAACTCGTTCCGACTTATTTATAGCAGAAAAAAACATC
GCTATTGGCAGTCGCTATTTAAGTCGTTTAATAAGGCACTATAACAATAATCGTATTTTAGCGTTTGCTGCTTATAATGC
GGGACCAACAATGGTGGCTGAATGGCGAAGGCTCGCAAGTGAAGAGCTCGATACGATAGGGTTTATAGAATCGATTTCCT
TTAAAGAAACTCGGACGTATGTGAAAAACATTTTAATGTTTGAAGAATATTATCGTCGTATATTAGGCATTAAAGATAAC
TTTATAAGTGATTTTGAATTGGATGCAAAATATTAA

Upstream 100 bases:

>100_bases
AAAGATCCTTAATTTTCAGTAAATGAGTATTAGATTCTAGGTATTTGCAAATATGGTACGTTTGAGTACTAGATAATTTG
ATATAACTAAACCAAACTCA

Downstream 100 bases:

>100_bases
CGTATGATTAAATATGGAAATAGAAGCATTGTCGTAAAAAAAACGAAGGAAATGAAATTAATGGTTTTGGGGTATTGAAT
TAAATGGTTACTTTAAATAC

Product: soluble lytic murein transglycosylase precursor

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MCAFMKKGFSMRSSKVHWLLAFVSICNVSYAFSASELRLNRYAYDAIQQGFNQKNISIYDKKRASIDSYSLAAYTDYRAF
LFDLPNKTPEQVNQFSKDYADYPFSVSIRADYLNAMIASKNWEALLRYQTTPPRDQDYQCYYYTAFYHQGKQEVAFEGAR
SLWLNGESVSNACNDLFSYWEKAGLRTDDVIKRRALLSIEERNPRLITYLIKLVSNKQSRVDLNEMYGLYQTPTKITTVF
DKKVETPFNREYAQVMLRHLARKDTDKAYLAFRFINDHYSYTKDELQTTSEFLAVRLFQTTDSETIEWRDDVFKRSNRDE
VLEQRIRLSIRKTQWENINFWILRLSDKAQSTYRWQYWMARSEIALEKVNEGEERLKRILGYRHIYSFAAAIALNREITY
PIASVDTKFEKIAQYHPDLERVKELLALGKDAAAKSQWRYLIARASYEDRARLATYARNEGWDEFSLESTFRGGLWDHIE
LRFPVRYYSLFTRYGGKYKVNPITLMSLTRQESGFNPNAHSPVGALGLMQVLPSTASYMATLYTLPYKTRSDLFIAEKNI
AIGSRYLSRLIRHYNNNRILAFAAYNAGPTMVAEWRRLASEELDTIGFIESISFKETRTYVKNILMFEEYYRRILGIKDN
FISDFELDAKY

Sequences:

>Translated_651_residues
MCAFMKKGFSMRSSKVHWLLAFVSICNVSYAFSASELRLNRYAYDAIQQGFNQKNISIYDKKRASIDSYSLAAYTDYRAF
LFDLPNKTPEQVNQFSKDYADYPFSVSIRADYLNAMIASKNWEALLRYQTTPPRDQDYQCYYYTAFYHQGKQEVAFEGAR
SLWLNGESVSNACNDLFSYWEKAGLRTDDVIKRRALLSIEERNPRLITYLIKLVSNKQSRVDLNEMYGLYQTPTKITTVF
DKKVETPFNREYAQVMLRHLARKDTDKAYLAFRFINDHYSYTKDELQTTSEFLAVRLFQTTDSETIEWRDDVFKRSNRDE
VLEQRIRLSIRKTQWENINFWILRLSDKAQSTYRWQYWMARSEIALEKVNEGEERLKRILGYRHIYSFAAAIALNREITY
PIASVDTKFEKIAQYHPDLERVKELLALGKDAAAKSQWRYLIARASYEDRARLATYARNEGWDEFSLESTFRGGLWDHIE
LRFPVRYYSLFTRYGGKYKVNPITLMSLTRQESGFNPNAHSPVGALGLMQVLPSTASYMATLYTLPYKTRSDLFIAEKNI
AIGSRYLSRLIRHYNNNRILAFAAYNAGPTMVAEWRRLASEELDTIGFIESISFKETRTYVKNILMFEEYYRRILGIKDN
FISDFELDAKY
>Mature_651_residues
MCAFMKKGFSMRSSKVHWLLAFVSICNVSYAFSASELRLNRYAYDAIQQGFNQKNISIYDKKRASIDSYSLAAYTDYRAF
LFDLPNKTPEQVNQFSKDYADYPFSVSIRADYLNAMIASKNWEALLRYQTTPPRDQDYQCYYYTAFYHQGKQEVAFEGAR
SLWLNGESVSNACNDLFSYWEKAGLRTDDVIKRRALLSIEERNPRLITYLIKLVSNKQSRVDLNEMYGLYQTPTKITTVF
DKKVETPFNREYAQVMLRHLARKDTDKAYLAFRFINDHYSYTKDELQTTSEFLAVRLFQTTDSETIEWRDDVFKRSNRDE
VLEQRIRLSIRKTQWENINFWILRLSDKAQSTYRWQYWMARSEIALEKVNEGEERLKRILGYRHIYSFAAAIALNREITY
PIASVDTKFEKIAQYHPDLERVKELLALGKDAAAKSQWRYLIARASYEDRARLATYARNEGWDEFSLESTFRGGLWDHIE
LRFPVRYYSLFTRYGGKYKVNPITLMSLTRQESGFNPNAHSPVGALGLMQVLPSTASYMATLYTLPYKTRSDLFIAEKNI
AIGSRYLSRLIRHYNNNRILAFAAYNAGPTMVAEWRRLASEELDTIGFIESISFKETRTYVKNILMFEEYYRRILGIKDN
FISDFELDAKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=647, Percent_Identity=31.839258114374, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76589; Mature: 76589

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAFMKKGFSMRSSKVHWLLAFVSICNVSYAFSASELRLNRYAYDAIQQGFNQKNISIYD
CCCCHHCCCCCCCCHHHHHHHHHHHHCCHHEECHHHHHHHHHHHHHHHCCCCCCCCEEEE
KKRASIDSYSLAAYTDYRAFLFDLPNKTPEQVNQFSKDYADYPFSVSIRADYLNAMIASK
CCCCCCCCCEEEEEHHHHHHEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
NWEALLRYQTTPPRDQDYQCYYYTAFYHQGKQEVAFEGARSLWLNGESVSNACNDLFSYW
CCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHCCHHEEECCHHHHHHHHHHHHHH
EKAGLRTDDVIKRRALLSIEERNPRLITYLIKLVSNKQSRVDLNEMYGLYQTPTKITTVF
HHCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHH
DKKVETPFNREYAQVMLRHLARKDTDKAYLAFRFINDHYSYTKDELQTTSEFLAVRLFQT
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEC
TDSETIEWRDDVFKRSNRDEVLEQRIRLSIRKTQWENINFWILRLSDKAQSTYRWQYWMA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHEEHH
RSEIALEKVNEGEERLKRILGYRHIYSFAAAIALNREITYPIASVDTKFEKIAQYHPDLE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHH
RVKELLALGKDAAAKSQWRYLIARASYEDRARLATYARNEGWDEFSLESTFRGGLWDHIE
HHHHHHHCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEE
LRFPVRYYSLFTRYGGKYKVNPITLMSLTRQESGFNPNAHSPVGALGLMQVLPSTASYMA
EEECHHHHHHHHHCCCEEECCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHH
TLYTLPYKTRSDLFIAEKNIAIGSRYLSRLIRHYNNNRILAFAAYNAGPTMVAEWRRLAS
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHH
EELDTIGFIESISFKETRTYVKNILMFEEYYRRILGIKDNFISDFELDAKY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCAFMKKGFSMRSSKVHWLLAFVSICNVSYAFSASELRLNRYAYDAIQQGFNQKNISIYD
CCCCHHCCCCCCCCHHHHHHHHHHHHCCHHEECHHHHHHHHHHHHHHHCCCCCCCCEEEE
KKRASIDSYSLAAYTDYRAFLFDLPNKTPEQVNQFSKDYADYPFSVSIRADYLNAMIASK
CCCCCCCCCEEEEEHHHHHHEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
NWEALLRYQTTPPRDQDYQCYYYTAFYHQGKQEVAFEGARSLWLNGESVSNACNDLFSYW
CCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHCCHHEEECCHHHHHHHHHHHHHH
EKAGLRTDDVIKRRALLSIEERNPRLITYLIKLVSNKQSRVDLNEMYGLYQTPTKITTVF
HHCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHH
DKKVETPFNREYAQVMLRHLARKDTDKAYLAFRFINDHYSYTKDELQTTSEFLAVRLFQT
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEC
TDSETIEWRDDVFKRSNRDEVLEQRIRLSIRKTQWENINFWILRLSDKAQSTYRWQYWMA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHEEHH
RSEIALEKVNEGEERLKRILGYRHIYSFAAAIALNREITYPIASVDTKFEKIAQYHPDLE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHH
RVKELLALGKDAAAKSQWRYLIARASYEDRARLATYARNEGWDEFSLESTFRGGLWDHIE
HHHHHHHCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEE
LRFPVRYYSLFTRYGGKYKVNPITLMSLTRQESGFNPNAHSPVGALGLMQVLPSTASYMA
EEECHHHHHHHHHCCCEEECCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHH
TLYTLPYKTRSDLFIAEKNIAIGSRYLSRLIRHYNNNRILAFAAYNAGPTMVAEWRRLAS
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHH
EELDTIGFIESISFKETRTYVKNILMFEEYYRRILGIKDNFISDFELDAKY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]