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The map label for this gene is yebU

Identifier: 209695043

GI number: 209695043

Start: 1638580

End: 1640001

Strand: Reverse

Name: yebU

Synonym: VSAL_I1538

Alternate gene names: 209695043

Gene position: 1640001-1638580 (Counterclockwise)

Preceding gene: 209695044

Following gene: 209695042

Centisome position: 49.32

GC content: 40.86

Gene sequence:

>1422_bases
GTGCACGATAATATTTTTCTACCCGATGCTTTTTTAGCACAAGTTCAAGAAACTATGCCTTCTCACCTTTCTATGGACGA
ATTTGTTGCCGCTTGTAAACGCCCTTTACGTCGCAGCATTCGTGTGAATACTCTAAAAAACAGTGTTGAAGAATTCAAAA
AAAGAGCCGAAGAAAAACAATGGCAATTAGACCCAGTGCCTTGGTGTGATACGGGATTTTGGATCACAAGACAAGAAAGC
GATACCGTAAAATTAGGCAGTACCGCTGAGCACATGGCTGGTCTATTTTACATTCAAGAAGCCAGTTCAATGATGCCCGT
CACTGCATTATTAAAGGACAATGACAATATTGAAATGGCATTAGACATGGCATCTGCTCCGGGATCAAAAACAACCCAAC
TTGCCGCTGGAATGAAAAACAAAGGCGCTTTAGTTGCGAATGAATATTCGTCAAGTCGTGTAAAAATATTGTGTTCAAAC
GTCCAGCGTTGTGGTGTGAGTAACGTAGCTCTTACTCATTTTGATGGTCGTGTATTTGGTGGTTGGTTACCTGAAACATT
TGATTCAATTCTACTGGATGCCCCTTGTTCTGGTGAAGGAACAATCCGTAAAGATCCTGACGCTATGCATAACTGGAGCC
CAGAATCGGTTATTGAAATTGGCGATACTCAACGTGATCTAATAAAAAGTGCGTTCCACGCTCTTAAGCCCGGTGGTGTT
ATGGTGTATTCAACTTGCACCCTAAACCATGAAGAAAACCAAAACATTTGTCATCATTTAGTGACCGAATTCGGTGACGC
CGTCACGTTCGAGCCATTAGGTGATCTTTTTGAGAATGCAGAAAAAGCATTAACAAAAGAAGGCTTTCTACATATCTACC
CTCAAATCTTTGACAGTGAAGGCTTCTTCGTTGCAAAGATTCGTAAAAACTCGGCAACGATTGCGCCGGAAGTTAAAAAA
CGATTAGGTAAGTTCCCTTTTGCTTTAGCAAATCAAAAAGAAATAAAAGCAATTGAAGATGAACTACATAACACGCTGCA
ATTATCTATTCCTGAAACCAATGAACTTTGGATCCGAGATAAAGAAGTATGGGCGTTTCCAAAAAGAATGAGCTCATTAA
TCGGTGAAATGCGTTATCACCGTATTGGATTCAAACTTGCAGAAACTCACAAGAAAGGCTATCGCTGGCAGCATGAAGCG
ATTATGGCTATTGCAAAAGCAGATAATCCAACAAGTTCTGAATTAACCATAGAACAAGCTCGTGAATGGTATATGGGTCG
TGATGTTCGTCCTGATAATGTGGGTAAAGGCGAAACCATTGTCACCTTTAACGGTGCCGTGATTGGATTAGGTAAATGGG
TTGGTAACCGCATAAAGAATGGGCTTCCTCGTGAACTGGTTAGAGACGGTAACTTATTTTAA

Upstream 100 bases:

>100_bases
ATTCTTTCAATAATTAAGTCTTTCAATTAATTCCATTTAGACAATAAGCTCAATAGGTATTACACTCCCTCCCATAGTCA
TCATCCAATGAGATCTCTTC

Downstream 100 bases:

>100_bases
TCAACGCTAGTTAAAAAGTAGTAGGGAAACGGATATGCCTTCACAATATAGAATCAAACGGATTGTTGAGATCGGAGACA
CCCTACACCGATGTGGGTGT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MHDNIFLPDAFLAQVQETMPSHLSMDEFVAACKRPLRRSIRVNTLKNSVEEFKKRAEEKQWQLDPVPWCDTGFWITRQES
DTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDNIEMALDMASAPGSKTTQLAAGMKNKGALVANEYSSSRVKILCSN
VQRCGVSNVALTHFDGRVFGGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIKSAFHALKPGGV
MVYSTCTLNHEENQNICHHLVTEFGDAVTFEPLGDLFENAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSATIAPEVKK
RLGKFPFALANQKEIKAIEDELHNTLQLSIPETNELWIRDKEVWAFPKRMSSLIGEMRYHRIGFKLAETHKKGYRWQHEA
IMAIAKADNPTSSELTIEQAREWYMGRDVRPDNVGKGETIVTFNGAVIGLGKWVGNRIKNGLPRELVRDGNLF

Sequences:

>Translated_473_residues
MHDNIFLPDAFLAQVQETMPSHLSMDEFVAACKRPLRRSIRVNTLKNSVEEFKKRAEEKQWQLDPVPWCDTGFWITRQES
DTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDNIEMALDMASAPGSKTTQLAAGMKNKGALVANEYSSSRVKILCSN
VQRCGVSNVALTHFDGRVFGGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIKSAFHALKPGGV
MVYSTCTLNHEENQNICHHLVTEFGDAVTFEPLGDLFENAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSATIAPEVKK
RLGKFPFALANQKEIKAIEDELHNTLQLSIPETNELWIRDKEVWAFPKRMSSLIGEMRYHRIGFKLAETHKKGYRWQHEA
IMAIAKADNPTSSELTIEQAREWYMGRDVRPDNVGKGETIVTFNGAVIGLGKWVGNRIKNGLPRELVRDGNLF
>Mature_473_residues
MHDNIFLPDAFLAQVQETMPSHLSMDEFVAACKRPLRRSIRVNTLKNSVEEFKKRAEEKQWQLDPVPWCDTGFWITRQES
DTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDNIEMALDMASAPGSKTTQLAAGMKNKGALVANEYSSSRVKILCSN
VQRCGVSNVALTHFDGRVFGGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIKSAFHALKPGGV
MVYSTCTLNHEENQNICHHLVTEFGDAVTFEPLGDLFENAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSATIAPEVKK
RLGKFPFALANQKEIKAIEDELHNTLQLSIPETNELWIRDKEVWAFPKRMSSLIGEMRYHRIGFKLAETHKKGYRWQHEA
IMAIAKADNPTSSELTIEQAREWYMGRDVRPDNVGKGETIVTFNGAVIGLGKWVGNRIKNGLPRELVRDGNLF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=305, Percent_Identity=34.7540983606557, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI76150623, Length=305, Percent_Identity=34.7540983606557, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI11545785, Length=230, Percent_Identity=30.8695652173913, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI32698918, Length=239, Percent_Identity=30.9623430962343, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI301336155, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI39995082, Length=189, Percent_Identity=31.2169312169312, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23199998, Length=304, Percent_Identity=27.9605263157895, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI40316918, Length=171, Percent_Identity=34.5029239766082, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8922322, Length=304, Percent_Identity=27.9605263157895, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI270288818, Length=304, Percent_Identity=27.9605263157895, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI270288816, Length=304, Percent_Identity=27.9605263157895, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87081985, Length=472, Percent_Identity=58.6864406779661, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI2367212, Length=237, Percent_Identity=29.957805907173, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=317, Percent_Identity=33.1230283911672, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25143471, Length=273, Percent_Identity=30.03663003663, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=302, Percent_Identity=25.4966887417219, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=325, Percent_Identity=35.0769230769231, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319447, Length=283, Percent_Identity=27.5618374558304, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI22024126, Length=311, Percent_Identity=34.0836012861736, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI21355201, Length=295, Percent_Identity=28.4745762711864, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24668781, Length=178, Percent_Identity=33.7078651685393, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356513, Length=178, Percent_Identity=33.7078651685393, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_ALISL (B6ELC0)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262972.1
- ProteinModelPortal:   B6ELC0
- GeneID:   6987183
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1538
- HOGENOM:   HBG726909
- OMA:   VANEYSA
- ProtClustDB:   PRK11933
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 53203; Mature: 53203

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 246-246 BINDING 148-148 BINDING 175-175 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDNIFLPDAFLAQVQETMPSHLSMDEFVAACKRPLRRSIRVNTLKNSVEEFKKRAEEKQ
CCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WQLDPVPWCDTGFWITRQESDTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDNIEMA
CCCCCCCCCCCCCEEEECCCCCEECCCCHHHHHHHHEEECCCCCCCEEEEEECCCCEEEE
LDMASAPGSKTTQLAAGMKNKGALVANEYSSSRVKILCSNVQRCGVSNVALTHFDGRVFG
EEECCCCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEC
GWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIKSAFHALKPGGV
CCCHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCCHHEECCCCHHHHHHHHHHHCCCCCE
MVYSTCTLNHEENQNICHHLVTEFGDAVTFEPLGDLFENAEKALTKEGFLHIYPQIFDSE
EEEEEEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHCCCEEECHHHCCCC
GFFVAKIRKNSATIAPEVKKRLGKFPFALANQKEIKAIEDELHNTLQLSIPETNELWIRD
CEEEEEEECCCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHCCEEEEECCCCCCEEEEC
KEVWAFPKRMSSLIGEMRYHRIGFKLAETHKKGYRWQHEAIMAIAKADNPTSSELTIEQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCEEHHHH
REWYMGRDVRPDNVGKGETIVTFNGAVIGLGKWVGNRIKNGLPRELVRDGNLF
HHHHCCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MHDNIFLPDAFLAQVQETMPSHLSMDEFVAACKRPLRRSIRVNTLKNSVEEFKKRAEEKQ
CCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WQLDPVPWCDTGFWITRQESDTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDNIEMA
CCCCCCCCCCCCCEEEECCCCCEECCCCHHHHHHHHEEECCCCCCCEEEEEECCCCEEEE
LDMASAPGSKTTQLAAGMKNKGALVANEYSSSRVKILCSNVQRCGVSNVALTHFDGRVFG
EEECCCCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEEC
GWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIKSAFHALKPGGV
CCCHHHHHHHEECCCCCCCCCCCCCCHHHHCCCCCHHEECCCCHHHHHHHHHHHCCCCCE
MVYSTCTLNHEENQNICHHLVTEFGDAVTFEPLGDLFENAEKALTKEGFLHIYPQIFDSE
EEEEEEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHCCCEEECHHHCCCC
GFFVAKIRKNSATIAPEVKKRLGKFPFALANQKEIKAIEDELHNTLQLSIPETNELWIRD
CEEEEEEECCCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHCCEEEEECCCCCCEEEEC
KEVWAFPKRMSSLIGEMRYHRIGFKLAETHKKGYRWQHEAIMAIAKADNPTSSELTIEQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCEEHHHH
REWYMGRDVRPDNVGKGETIVTFNGAVIGLGKWVGNRIKNGLPRELVRDGNLF
HHHHCCCCCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA