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The map label for this gene is asnO [H]

Identifier: 209694839

GI number: 209694839

Start: 1416608

End: 1417630

Strand: Reverse

Name: asnO [H]

Synonym: VSAL_I1305

Alternate gene names: 209694839

Gene position: 1417630-1416608 (Counterclockwise)

Preceding gene: 209694842

Following gene: 209694838

Centisome position: 42.63

GC content: 34.6

Gene sequence:

>1023_bases
GTGTTCAATTTAAAAAAAGAAAGCAGCCAAGAGCTGCTAAAAATAATAGAAATTCATATTCCTTACGATTCAGCAAGGAA
ATTAAGCAGCGCATTTTCTCACTTGAGATTAGGAGATGACAAATCATGGCTAAGAACTAGTCATTGGTTCGGAACTACAA
TAAAGCATTGTTTAATAAAAGAACTTAATTATAAAATTTCTAATTTCAAAGAAAATAAAGATCAACAGGCATTAATTATT
AGAGGCTTACCTATTGATCAAAACCTTTGTAAAACACCATATAATGGCTATGTTTCACCAAGTAAATTACCCATCATCAG
CGCCATTAACATTGGCATATATCAATTAGCTAAAATTGAACCAACTTCATATCAAAGTGAAAACGATGGACTGCTATTTA
GACATGTCGTTCCATCACTAAAAGGGCGAAATGATAAATCCTCACACGGTTCAAAACATACTTTTGGTCATCATGTCGAT
AATCCTGATCTTCCCCTTACTAATGAAAAAATAACAGATAAAAGTGGCTGTCCAGAGTTCCTATCTCTAATGTCACTACG
CTCAGATCTAAAAGTTAAATCTAATTTCATACTCGTTGATGACGTACTTAATCAATTAAGTAACGGAGTTATTGAACAGT
TGAGTAAACCTCACTTTGAAATTTCAAGACCGGATTCATTTAAACAATCTGTTAAAACAATTCTCCCACTTATCAGCTTT
GATAATGATGGAGTGGCCTACTGCCGTTACGACAAAGAAAATACAACACCACTCACAACAGAAGCTGCAGCCGCTCTTGT
TATGTGGGAAGCACAACTCAAAAATACAGAGTTAAATAATGCCATTACTTATCAACCTGGCGATTTCTTAATCATAAAAA
ATCAACGCTTAATGCATAGCAGAGAAGGATTTTCACCACGAGATGACGGAACAGATCGCTGGTTAATTCGCTTATTCGGC
ATGAGCTCATTAGAACGAATTATCCCTATTAATGAAGAAAATAAACATATTGCACAAGACTAG

Upstream 100 bases:

>100_bases
TTATGAATGAAATACTTTAATACAATTTAATTGACATATGTCACTAGGTTGTTTTATAGTCAACTCTCAGTTGGTAATTA
ACATATAGGACATACGGAAA

Downstream 100 bases:

>100_bases
GAGCATAGAATGGAAGCACAAAAAGTAGCATTTATTGGATTAGGTGTAATGGGATACCCAATGGCTGGGCACTTACAACA
CTCAGGACTAGAGACAACTG

Product: hypothetical protein

Products: NA

Alternate protein names: L-asparagine 3-hydroxylase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MFNLKKESSQELLKIIEIHIPYDSARKLSSAFSHLRLGDDKSWLRTSHWFGTTIKHCLIKELNYKISNFKENKDQQALII
RGLPIDQNLCKTPYNGYVSPSKLPIISAINIGIYQLAKIEPTSYQSENDGLLFRHVVPSLKGRNDKSSHGSKHTFGHHVD
NPDLPLTNEKITDKSGCPEFLSLMSLRSDLKVKSNFILVDDVLNQLSNGVIEQLSKPHFEISRPDSFKQSVKTILPLISF
DNDGVAYCRYDKENTTPLTTEAAAALVMWEAQLKNTELNNAITYQPGDFLIIKNQRLMHSREGFSPRDDGTDRWLIRLFG
MSSLERIIPINEENKHIAQD

Sequences:

>Translated_340_residues
MFNLKKESSQELLKIIEIHIPYDSARKLSSAFSHLRLGDDKSWLRTSHWFGTTIKHCLIKELNYKISNFKENKDQQALII
RGLPIDQNLCKTPYNGYVSPSKLPIISAINIGIYQLAKIEPTSYQSENDGLLFRHVVPSLKGRNDKSSHGSKHTFGHHVD
NPDLPLTNEKITDKSGCPEFLSLMSLRSDLKVKSNFILVDDVLNQLSNGVIEQLSKPHFEISRPDSFKQSVKTILPLISF
DNDGVAYCRYDKENTTPLTTEAAAALVMWEAQLKNTELNNAITYQPGDFLIIKNQRLMHSREGFSPRDDGTDRWLIRLFG
MSSLERIIPINEENKHIAQD
>Mature_340_residues
MFNLKKESSQELLKIIEIHIPYDSARKLSSAFSHLRLGDDKSWLRTSHWFGTTIKHCLIKELNYKISNFKENKDQQALII
RGLPIDQNLCKTPYNGYVSPSKLPIISAINIGIYQLAKIEPTSYQSENDGLLFRHVVPSLKGRNDKSSHGSKHTFGHHVD
NPDLPLTNEKITDKSGCPEFLSLMSLRSDLKVKSNFILVDDVLNQLSNGVIEQLSKPHFEISRPDSFKQSVKTILPLISF
DNDGVAYCRYDKENTTPLTTEAAAALVMWEAQLKNTELNNAITYQPGDFLIIKNQRLMHSREGFSPRDDGTDRWLIRLFG
MSSLERIIPINEENKHIAQD

Specific function: Catalyzes the 3-hydroxylation of L-asparagine to (2S,3S)-3-hydroxyasparagine. The 3-hydroxylated asparagine produced is incorporated at position 9 during the biosynthesis of the non-ribosomally synthesized calcium-dependent antibiotic (CDA), a 11-residue

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clavaminate synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014503
- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: NA

Molecular weight: Translated: 38736; Mature: 38736

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLKKESSQELLKIIEIHIPYDSARKLSSAFSHLRLGDDKSWLRTSHWFGTTIKHCLIK
CCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELNYKISNFKENKDQQALIIRGLPIDQNLCKTPYNGYVSPSKLPIISAINIGIYQLAKIE
HHCCHHHCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEECCCEEEEEEEC
PTSYQSENDGLLFRHVVPSLKGRNDKSSHGSKHTFGHHVDNPDLPLTNEKITDKSGCPEF
CCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LSLMSLRSDLKVKSNFILVDDVLNQLSNGVIEQLSKPHFEISRPDSFKQSVKTILPLISF
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHEE
DNDGVAYCRYDKENTTPLTTEAAAALVMWEAQLKNTELNNAITYQPGDFLIIKNQRLMHS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHH
REGFSPRDDGTDRWLIRLFGMSSLERIIPINEENKHIAQD
CCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFNLKKESSQELLKIIEIHIPYDSARKLSSAFSHLRLGDDKSWLRTSHWFGTTIKHCLIK
CCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELNYKISNFKENKDQQALIIRGLPIDQNLCKTPYNGYVSPSKLPIISAINIGIYQLAKIE
HHCCHHHCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEECCCEEEEEEEC
PTSYQSENDGLLFRHVVPSLKGRNDKSSHGSKHTFGHHVDNPDLPLTNEKITDKSGCPEF
CCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LSLMSLRSDLKVKSNFILVDDVLNQLSNGVIEQLSKPHFEISRPDSFKQSVKTILPLISF
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHEE
DNDGVAYCRYDKENTTPLTTEAAAALVMWEAQLKNTELNNAITYQPGDFLIIKNQRLMHS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHH
REGFSPRDDGTDRWLIRLFGMSSLERIIPINEENKHIAQD
CCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]