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The map label for this gene is garR [C]

Identifier: 209694838

GI number: 209694838

Start: 1415702

End: 1416598

Strand: Reverse

Name: garR [C]

Synonym: VSAL_I1304

Alternate gene names: 209694838

Gene position: 1416598-1415702 (Counterclockwise)

Preceding gene: 209694839

Following gene: 209694837

Centisome position: 42.6

GC content: 38.91

Gene sequence:

>897_bases
ATGGAAGCACAAAAAGTAGCATTTATTGGATTAGGTGTAATGGGATACCCAATGGCTGGGCACTTACAACACTCAGGACT
AGAGACAACTGTTTATAACCGTTCTTTAGATAAAGCCCTTCAATGGTGTACTGAATACCAAGGTTTTTTAGCTTCAAGCC
CTGCTGAAGCAGCTAAGAACTGTGACATTGTATTTATTTGCGTAGGAAATGACGATGATGTCCGAAGCGTTGTTTATGGG
CCAGAAGGCGTACTGGAAGGAATGAAAGAAAATGCCATTCTTGTTGATCATACAACAGTTTCAGCTGACTTAGCGATAGA
GCTTGCGCAACAGTGCAAACAAAAAAAGATCAACTTTATTGATGCTCCTGTTTCTGGGGGGCAAGCTGGTGCAGAAAATG
GAACACTTACTATTATGTGCGGCGGAGCAAAAGAAACCTTCGACCAAATTGTTACTACAATAGATTGCTATGCAAAACAA
GCAACACTATTAGGAGATAATGGGCAAGGACAACGATGCAAAATGGTTAATCAGATTTGTATCGCTGGTGTATTAAAAGG
ACTAAGCGAAGCTCTACTTTTAGCAGAAAAATCAAATCTTGATATTGAACAAGTAGTTAACGTACTTAAGCACGGTGCTG
CTGGCTCATGGCAAATGGAAAACCGAGCAGTGACTATGGCTGAGCGTAAATTTGATTTCGGCTTTGCTATTGATTGGATG
AGAAAAGATCTCTCTATTTGCTTAAATGAAGCTAAGCAACACAATTTATCTCTACCTATGACACAAGAGATTGATAGGTC
ATACGCGATCTTACAAGATCTCAATCTTGGTCGTATGGATACCTCAATTTTGATTAAAAGTTATGATCAACCTATTACAA
ATGAATTAGCAATTTAA

Upstream 100 bases:

>100_bases
AGATCGCTGGTTAATTCGCTTATTCGGCATGAGCTCATTAGAACGAATTATCCCTATTAATGAAGAAAATAAACATATTG
CACAAGACTAGGAGCATAGA

Downstream 100 bases:

>100_bases
GGAGGCAATAATGGAATGGATAAGTAATGACATCACACTATGGATTTTAATCGCGTTAATGTTATCTGCATTTGCAGCTG
GATTCATTGACAGTGTTGCA

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MEAQKVAFIGLGVMGYPMAGHLQHSGLETTVYNRSLDKALQWCTEYQGFLASSPAEAAKNCDIVFICVGNDDDVRSVVYG
PEGVLEGMKENAILVDHTTVSADLAIELAQQCKQKKINFIDAPVSGGQAGAENGTLTIMCGGAKETFDQIVTTIDCYAKQ
ATLLGDNGQGQRCKMVNQICIAGVLKGLSEALLLAEKSNLDIEQVVNVLKHGAAGSWQMENRAVTMAERKFDFGFAIDWM
RKDLSICLNEAKQHNLSLPMTQEIDRSYAILQDLNLGRMDTSILIKSYDQPITNELAI

Sequences:

>Translated_298_residues
MEAQKVAFIGLGVMGYPMAGHLQHSGLETTVYNRSLDKALQWCTEYQGFLASSPAEAAKNCDIVFICVGNDDDVRSVVYG
PEGVLEGMKENAILVDHTTVSADLAIELAQQCKQKKINFIDAPVSGGQAGAENGTLTIMCGGAKETFDQIVTTIDCYAKQ
ATLLGDNGQGQRCKMVNQICIAGVLKGLSEALLLAEKSNLDIEQVVNVLKHGAAGSWQMENRAVTMAERKFDFGFAIDWM
RKDLSICLNEAKQHNLSLPMTQEIDRSYAILQDLNLGRMDTSILIKSYDQPITNELAI
>Mature_298_residues
MEAQKVAFIGLGVMGYPMAGHLQHSGLETTVYNRSLDKALQWCTEYQGFLASSPAEAAKNCDIVFICVGNDDDVRSVVYG
PEGVLEGMKENAILVDHTTVSADLAIELAQQCKQKKINFIDAPVSGGQAGAENGTLTIMCGGAKETFDQIVTTIDCYAKQ
ATLLGDNGQGQRCKMVNQICIAGVLKGLSEALLLAEKSNLDIEQVVNVLKHGAAGSWQMENRAVTMAERKFDFGFAIDWM
RKDLSICLNEAKQHNLSLPMTQEIDRSYAILQDLNLGRMDTSILIKSYDQPITNELAI

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=27.0175438596491, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI23308751, Length=256, Percent_Identity=28.515625, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI145693186, Length=286, Percent_Identity=32.5174825174825, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786719, Length=299, Percent_Identity=33.4448160535117, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=30.4498269896194, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789092, Length=288, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=27.4725274725275, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=30.2721088435374, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=30.2721088435374, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=25.4355400696864, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32474; Mature: 32474

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAQKVAFIGLGVMGYPMAGHLQHSGLETTVYNRSLDKALQWCTEYQGFLASSPAEAAKN
CCCCEEEEEECCHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
CDIVFICVGNDDDVRSVVYGPEGVLEGMKENAILVDHTTVSADLAIELAQQCKQKKINFI
CCEEEEEECCCCCCHHEEECCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
DAPVSGGQAGAENGTLTIMCGGAKETFDQIVTTIDCYAKQATLLGDNGQGQRCKMVNQIC
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
IAGVLKGLSEALLLAEKSNLDIEQVVNVLKHGAAGSWQMENRAVTMAERKFDFGFAIDWM
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEHHHCCCCCCHHHHH
RKDLSICLNEAKQHNLSLPMTQEIDRSYAILQDLNLGRMDTSILIKSYDQPITNELAI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MEAQKVAFIGLGVMGYPMAGHLQHSGLETTVYNRSLDKALQWCTEYQGFLASSPAEAAKN
CCCCEEEEEECCHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
CDIVFICVGNDDDVRSVVYGPEGVLEGMKENAILVDHTTVSADLAIELAQQCKQKKINFI
CCEEEEEECCCCCCHHEEECCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
DAPVSGGQAGAENGTLTIMCGGAKETFDQIVTTIDCYAKQATLLGDNGQGQRCKMVNQIC
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
IAGVLKGLSEALLLAEKSNLDIEQVVNVLKHGAAGSWQMENRAVTMAERKFDFGFAIDWM
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEHHHCCCCCCHHHHH
RKDLSICLNEAKQHNLSLPMTQEIDRSYAILQDLNLGRMDTSILIKSYDQPITNELAI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA