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The map label for this gene is ampC [H]

Identifier: 209694787

GI number: 209694787

Start: 1354293

End: 1355156

Strand: Reverse

Name: ampC [H]

Synonym: VSAL_I1245

Alternate gene names: 209694787

Gene position: 1355156-1354293 (Counterclockwise)

Preceding gene: 209694788

Following gene: 209694786

Centisome position: 40.75

GC content: 40.16

Gene sequence:

>864_bases
ATGATACGTAAGACGGTTTTGACCCCATTTTTTATCTTATTTATGCTATTCGCCAACACCTCAATGGCGGCATCACTTAA
TGACACTATGATCGATCTTGAAAAACAAACATCCGGTGACATTGGTGTGGCCGTTTTAGATACCAAAACAAACCAAATAT
GGAGCTATAAAGGCAATCAACGATTTCCAATGATGAGCACCTTTAAAACACTTGCTTGTGCGAAGATGCTGCATGACGCA
GACAAAAAAATCATTAATAAAAAAACTGAAACAGCCATCGCACAAAAGGATTTAATCCCTTGGTCTCCAATAACAGAAAA
GTACGTAGGTAAAAGCATTTCTACAGAAAAAGCCTGTGAAGCCACAATGCTCATGAGTGATAACACCGCCGCCAATATCG
TATTACAGCAAATAGGCAGCCCAAGTTCGCTAACTGCTTTTCTTCGATTAATGGGTGATAGCACGACACAACTGGATCGC
ATTGAACCTACATTGAATGAGGCAAAAAAAGGCGATATCAGAGACACAACAACACCTATTGCAATGGTTCATACCATTAA
CAAACTTCTGTTAGGTAATACACTGACCACTACCGATAAAGTGACACTGAAAAAGTGGATGATGGATAACAAGGTGTCTG
ATCCCCTATTACGATCTATTTTACCAATACATTGGTCTATTGCTGATCGCTCAGGGGCTGGAGGTTATGGGTCTCGTGGG
ATCACCGCAATTATTTGGAATGAACATCGCTTACCGTTGATCATCAGTATTTATCTAACTCAAACGGACCTTACATTGGC
AGAGCGTAATGACATTATCGTTCACGTTGGCTCCGCATTATTTACACAATTCAACGTAAAATAG

Upstream 100 bases:

>100_bases
AACAATCCCAATACCACACTTGTTTATATCCACCAAGGCGGCTTGTTAGGTAATGAAAGCATGCTACCGAGATACATAAG
AAAATATGAGAACTAATGAC

Downstream 100 bases:

>100_bases
AACTCGTGAAGCCAAAGTTCACTTAATTAACGCCTCGGTTCACTGATTCTCGTTTTATGAATAATGCCTTTTCTATATAG
CTTGAAGAGGTAAATGCGTA

Product: beta-lactamase

Products: NA

Alternate protein names: Carbenicillinase 4 [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MIRKTVLTPFFILFMLFANTSMAASLNDTMIDLEKQTSGDIGVAVLDTKTNQIWSYKGNQRFPMMSTFKTLACAKMLHDA
DKKIINKKTETAIAQKDLIPWSPITEKYVGKSISTEKACEATMLMSDNTAANIVLQQIGSPSSLTAFLRLMGDSTTQLDR
IEPTLNEAKKGDIRDTTTPIAMVHTINKLLLGNTLTTTDKVTLKKWMMDNKVSDPLLRSILPIHWSIADRSGAGGYGSRG
ITAIIWNEHRLPLIISIYLTQTDLTLAERNDIIVHVGSALFTQFNVK

Sequences:

>Translated_287_residues
MIRKTVLTPFFILFMLFANTSMAASLNDTMIDLEKQTSGDIGVAVLDTKTNQIWSYKGNQRFPMMSTFKTLACAKMLHDA
DKKIINKKTETAIAQKDLIPWSPITEKYVGKSISTEKACEATMLMSDNTAANIVLQQIGSPSSLTAFLRLMGDSTTQLDR
IEPTLNEAKKGDIRDTTTPIAMVHTINKLLLGNTLTTTDKVTLKKWMMDNKVSDPLLRSILPIHWSIADRSGAGGYGSRG
ITAIIWNEHRLPLIISIYLTQTDLTLAERNDIIVHVGSALFTQFNVK
>Mature_287_residues
MIRKTVLTPFFILFMLFANTSMAASLNDTMIDLEKQTSGDIGVAVLDTKTNQIWSYKGNQRFPMMSTFKTLACAKMLHDA
DKKIINKKTETAIAQKDLIPWSPITEKYVGKSISTEKACEATMLMSDNTAANIVLQQIGSPSSLTAFLRLMGDSTTQLDR
IEPTLNEAKKGDIRDTTTPIAMVHTINKLLLGNTLTTTDKVTLKKWMMDNKVSDPLLRSILPIHWSIADRSGAGGYGSRG
ITAIIWNEHRLPLIISIYLTQTDLTLAERNDIIVHVGSALFTQFNVK

Specific function: Hydrolyzes carbenicillin. Methicillin and oxacillin are weakly hydrolyzed [H]

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 31885; Mature: 31885

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKTVLTPFFILFMLFANTSMAASLNDTMIDLEKQTSGDIGVAVLDTKTNQIWSYKGNQ
CCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCC
RFPMMSTFKTLACAKMLHDADKKIINKKTETAIAQKDLIPWSPITEKYVGKSISTEKACE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHH
ATMLMSDNTAANIVLQQIGSPSSLTAFLRLMGDSTTQLDRIEPTLNEAKKGDIRDTTTPI
EEEEECCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCCCCCCCHH
AMVHTINKLLLGNTLTTTDKVTLKKWMMDNKVSDPLLRSILPIHWSIADRSGAGGYGSRG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCC
ITAIIWNEHRLPLIISIYLTQTDLTLAERNDIIVHVGSALFTQFNVK
EEEEEECCCCCCEEEEEEEEECCEEEECCCCEEEEECHHHHHEECCC
>Mature Secondary Structure
MIRKTVLTPFFILFMLFANTSMAASLNDTMIDLEKQTSGDIGVAVLDTKTNQIWSYKGNQ
CCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCC
RFPMMSTFKTLACAKMLHDADKKIINKKTETAIAQKDLIPWSPITEKYVGKSISTEKACE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHH
ATMLMSDNTAANIVLQQIGSPSSLTAFLRLMGDSTTQLDRIEPTLNEAKKGDIRDTTTPI
EEEEECCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCCCCCCCHH
AMVHTINKLLLGNTLTTTDKVTLKKWMMDNKVSDPLLRSILPIHWSIADRSGAGGYGSRG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCC
ITAIIWNEHRLPLIISIYLTQTDLTLAERNDIIVHVGSALFTQFNVK
EEEEEECCCCCCEEEEEEEEECCEEEECCCCEEEEECHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9687391 [H]