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The map label for this gene is nemR [H]

Identifier: 209694784

GI number: 209694784

Start: 1351891

End: 1352463

Strand: Reverse

Name: nemR [H]

Synonym: VSAL_I1242

Alternate gene names: 209694784

Gene position: 1352463-1351891 (Counterclockwise)

Preceding gene: 209694785

Following gene: 209694776

Centisome position: 40.67

GC content: 38.22

Gene sequence:

>573_bases
ATGAAAGACATGCGACAATCTATGCTCGATGCAGGATTCAAACTAATTAATGAACATGGTTTTGCTGGCGTAGGCTTGAT
GAAAATCATCAATGAAGCCCAAGGAACGAAAGGTTCTTTTTATCATTACTTCAAATCTAAAGAACATTTTGGAGAGACTT
TACTTAGTGATTATTTTGAAGAACACTTAGCGATCTTAGAAACATTTCTAAGTGATGATTCAATGACTCGCAATGAGCGT
GTAAAAGCATACTTCCAGTACTGGGCAACCTCTAAATTGACTGATGATTTCCATATTAAATGCCTCGTGGTAAAACTGGC
AGGTGAAATCTCTGGAGCGTCTGGTAAGATGCAGATGGTTATGGCTGAAGGTGCAGAAAAAGTGATTCAGCGTATGGCTC
AGTTCTTTGAACAAGGTAATACTGAGAACGATTTTACTATCCAAAATCCAGAAGACACCTCTCGTATGATATATAGCTTA
TGGCTTGGTAGCACACTGTTAGCAGCAATGCAGAAACGCAGAGAGATCCTTAATAACGCAATGAAAGACACGCTAATCAT
TCTTAAATGTTAA

Upstream 100 bases:

>100_bases
CAACCTTTTTTATTGACACATTTTCAACCAAATTAAACTAGACGATTGCTATACTAGCCAGTTACACTTTCTAGCTTACT
TTGGTCTCGGGTAAATAACG

Downstream 100 bases:

>100_bases
TCAACCTTATTAAAAATAATTTCAATAAATAAAGCAAGAGCATAATTAGTCGAATAAACATTCGATTATTAGTATCAATG
AAAAACAGGTCACTTTATTA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKDMRQSMLDAGFKLINEHGFAGVGLMKIINEAQGTKGSFYHYFKSKEHFGETLLSDYFEEHLAILETFLSDDSMTRNER
VKAYFQYWATSKLTDDFHIKCLVVKLAGEISGASGKMQMVMAEGAEKVIQRMAQFFEQGNTENDFTIQNPEDTSRMIYSL
WLGSTLLAAMQKRREILNNAMKDTLIILKC

Sequences:

>Translated_190_residues
MKDMRQSMLDAGFKLINEHGFAGVGLMKIINEAQGTKGSFYHYFKSKEHFGETLLSDYFEEHLAILETFLSDDSMTRNER
VKAYFQYWATSKLTDDFHIKCLVVKLAGEISGASGKMQMVMAEGAEKVIQRMAQFFEQGNTENDFTIQNPEDTSRMIYSL
WLGSTLLAAMQKRREILNNAMKDTLIILKC
>Mature_190_residues
MKDMRQSMLDAGFKLINEHGFAGVGLMKIINEAQGTKGSFYHYFKSKEHFGETLLSDYFEEHLAILETFLSDDSMTRNER
VKAYFQYWATSKLTDDFHIKCLVVKLAGEISGASGKMQMVMAEGAEKVIQRMAQFFEQGNTENDFTIQNPEDTSRMIYSL
WLGSTLLAAMQKRREILNNAMKDTLIILKC

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=180, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 21752; Mature: 21752

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDMRQSMLDAGFKLINEHGFAGVGLMKIINEAQGTKGSFYHYFKSKEHFGETLLSDYFE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EHLAILETFLSDDSMTRNERVKAYFQYWATSKLTDDFHIKCLVVKLAGEISGASGKMQMV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEHHHCCCCCCCHHHH
MAEGAEKVIQRMAQFFEQGNTENDFTIQNPEDTSRMIYSLWLGSTLLAAMQKRREILNNA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKDTLIILKC
HHHHEEEEEC
>Mature Secondary Structure
MKDMRQSMLDAGFKLINEHGFAGVGLMKIINEAQGTKGSFYHYFKSKEHFGETLLSDYFE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EHLAILETFLSDDSMTRNERVKAYFQYWATSKLTDDFHIKCLVVKLAGEISGASGKMQMV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEHHHCCCCCCCHHHH
MAEGAEKVIQRMAQFFEQGNTENDFTIQNPEDTSRMIYSLWLGSTLLAAMQKRREILNNA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKDTLIILKC
HHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]