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The map label for this gene is purA2 [H]

Identifier: 209694741

GI number: 209694741

Start: 1302210

End: 1303466

Strand: Reverse

Name: purA2 [H]

Synonym: VSAL_I1195

Alternate gene names: 209694741

Gene position: 1303466-1302210 (Counterclockwise)

Preceding gene: 209694745

Following gene: 209694738

Centisome position: 39.2

GC content: 44.15

Gene sequence:

>1257_bases
ATGCCGTCTATTGTTGTTGTTGGTGCTAATTGGGGCGATGAAGGTAAAGGCCGTATCGTTGATTTTCTTGCTGCAGATGC
ATCTGCAAGTATTCGTTTTCAGGGCGGTAATAACGCCGGTCATACTGTGGTAAATGACTTCGGTACGTTTAAATTACACC
AACTACCACGCGGTATTTTTAACCCAGACTGTACAGCGGTTCTTGGCCCTGGCATGGTAATTAGCCCTGCAGCACTAAGC
GAAGAAATCGCAGAAGTTAAAGCCGCTGGCATTGAAGTAAAAATGCACATTTCTGACCGTGCTACGCTTTGCCTTCCTCT
TCATGCGCTTGAAGATACTCTTGAAGAATTACGTTTAGGCGATGGCGCTTATGGTTCAACTCGTCAAGGTATTGCACCTG
CGTACGGTGACCGTGTAATGAAAAAAGGCATCCTTGTTGGTTGGTTAATGCAGCCTGAAGTATTGCTTGAGCGTATTCAA
TTCATGCTTGATTGGAAAATGCCACAACTGAAAGCACTATATCCAACATGTGATTTCAGCCAAACCGCTGAAGAAATGAC
AGAATGGCTTTTAGAAGTAACTGCACCTTGGCGTAAATTCATCTGTAACGTGACTGAGCCTCTAAAAGTAATGCAAAAGA
ACGATGCAAACTTATTGTTTGAAGCACAGTTAGGCGCGGGTCGTGACTTAGTTTACGGTGAATACCCATGGACTACTTCT
TCAAACGTAACAGCAGCTTATGCTGGTATCGGTAGTGGCTTACCAGCGTTACGTCCTGAGCGTATTATTGCAGTTGCTAA
GTCATTCAGCTCATCTGTTGGTACTGGTACATTAGTAACCGCAATGGAAGAGCAAGATAACTTCCGTGAAGCAGCTAATG
AATATGGCGCAGTAACAGGTCGTCCACGTGATATGGGTTACTTTGATGCAGTAGCAACACGCAACGGTGTTGAACTACAA
GCAGCAACAGAAATCGCATTAACAAAAATCGACTGTTTAAGTGGTATGGCGGATCTTAAAATCTGTACGTCTTATGCTGG
CGAACACACTGAGAACCCAATCTGGCCTCAAACTGCTGCATTAAGCCCTGTATATGAAAACATGGAAGCATGGCAAGAAG
ACATCACTGGCTGTCGTACTTTTGAAAGCCTACCAAAAGCAGCTCAAGCGTATGTATTACGTATTGAAGAGTTAATGGGC
GTACCAGTAAGTATGGTGTCAGTAGGTCCTGAACGTGAGCAAATGATCCTTCGTTAA

Upstream 100 bases:

>100_bases
ATGATATATGGATTTCTTATCAAGTCGATAATTATAATTAATTTCTCATATTGATTTATTAGGATTAACATAGCGTCTTA
ATCATTTAGGAGTTTGTGTT

Downstream 100 bases:

>100_bases
TCGAGTCATATAAACCACGTACATTTCCAAACAAAGAATTAGAAAGCCGCACTCCCCTATTTTGTGAGAATTTTAGGGAG
TGCGGCTTTTTTATATCAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPSIVVVGANWGDEGKGRIVDFLAADASASIRFQGGNNAGHTVVNDFGTFKLHQLPRGIFNPDCTAVLGPGMVISPAALS
EEIAEVKAAGIEVKMHISDRATLCLPLHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLMQPEVLLERIQ
FMLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWRKFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERIIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMADLKICTSYAGEHTENPIWPQTAALSPVYENMEAWQEDITGCRTFESLPKAAQAYVLRIEELMG
VPVSMVSVGPEREQMILR

Sequences:

>Translated_418_residues
MPSIVVVGANWGDEGKGRIVDFLAADASASIRFQGGNNAGHTVVNDFGTFKLHQLPRGIFNPDCTAVLGPGMVISPAALS
EEIAEVKAAGIEVKMHISDRATLCLPLHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLMQPEVLLERIQ
FMLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWRKFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERIIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMADLKICTSYAGEHTENPIWPQTAALSPVYENMEAWQEDITGCRTFESLPKAAQAYVLRIEELMG
VPVSMVSVGPEREQMILR
>Mature_417_residues
PSIVVVGANWGDEGKGRIVDFLAADASASIRFQGGNNAGHTVVNDFGTFKLHQLPRGIFNPDCTAVLGPGMVISPAALSE
EIAEVKAAGIEVKMHISDRATLCLPLHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLMQPEVLLERIQF
MLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWRKFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTSS
NVTAAYAGIGSGLPALRPERIIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQA
ATEIALTKIDCLSGMADLKICTSYAGEHTENPIWPQTAALSPVYENMEAWQEDITGCRTFESLPKAAQAYVLRIEELMGV
PVSMVSVGPEREQMILR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=33.7931034482759, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=33.4106728538283, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=31.0945273631841, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=36.214953271028, Blast_Score=270, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=32.6388888888889, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=32.6388888888889, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=29.9086757990868, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=33.4117647058824, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45547; Mature: 45415

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGANWGDEGKGRIVDFLAADASASIRFQGGNNAGHTVVNDFGTFKLHQLPRGIF
CCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEECCCCCEEHHHCCCCCC
NPDCTAVLGPGMVISPAALSEEIAEVKAAGIEVKMHISDRATLCLPLHALEDTLEELRLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLMQPEVLLERIQFMLDWKMPQLKALYPTCDFS
CCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCHHHEECCCCCCH
QTAEEMTEWLLEVTAPWRKFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERIIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMADLKICTSYAGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHH
LSPVYENMEAWQEDITGCRTFESLPKAAQAYVLRIEELMGVPVSMVSVGPEREQMILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
>Mature Secondary Structure 
PSIVVVGANWGDEGKGRIVDFLAADASASIRFQGGNNAGHTVVNDFGTFKLHQLPRGIF
CCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEECCCCCEEHHHCCCCCC
NPDCTAVLGPGMVISPAALSEEIAEVKAAGIEVKMHISDRATLCLPLHALEDTLEELRLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLMQPEVLLERIQFMLDWKMPQLKALYPTCDFS
CCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCHHHEECCCCCCH
QTAEEMTEWLLEVTAPWRKFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERIIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMADLKICTSYAGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHH
LSPVYENMEAWQEDITGCRTFESLPKAAQAYVLRIEELMGVPVSMVSVGPEREQMILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA