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The map label for this gene is rluB [H]

Identifier: 209694708

GI number: 209694708

Start: 1262544

End: 1263608

Strand: Direct

Name: rluB [H]

Synonym: VSAL_I1159

Alternate gene names: 209694708

Gene position: 1262544-1263608 (Clockwise)

Preceding gene: 209694705

Following gene: 209694710

Centisome position: 37.97

GC content: 42.44

Gene sequence:

>1065_bases
ATGAGCGAAAAATTACAGAAAGTATTAGCACGAGCTGGTCTTGGTTCTCGTCGTGAGTTAGAAACGATGATTCAAGCTGG
TCGAGTTAGTATTGATGGTCAGAAAGCAAAATTAGGCGATCGCCTAGAAAACGTTGATGCAAGAATCCGCATCGATGGCC
ATGTAGTATCAGTAAAAGAATCTACTGATGTGATCTGTCGAGTGCTTGCTTACCACAAGCCTGAAGGCGAATTATGTACA
CGTCATGATCCAGAAGGTCGTCGTACTGTTTTTGACCGTTTACCAAAAATTAAAGATTCTCGTTGGGTATCTGTTGGTCG
TCTGGATGCCAACACTGCGGGTCTATTGTTGTTTACAACAGATGGTGAACTTGCAAACCGTCTAATGCACCCAAGCCGCA
AAGTTGAACGTGAATATATGTGTCGTATATTCGGTGACATTAACGACAAAATGGTTAAAAACCTAGTTACTGGTGTAAAA
CTAGAAGATGGTGAAGCTCGTTTTGAAGATGTAATGTACGCCGGTGGCGAAGGCATGAACCACACTTTCTATGTTGTGAT
TAATGAAGGTCGTAACCGTGAAGTTCGTCGTTTATGGGATTCACAAGGCGTAACAGTAAGTCGTCTAAAACGTGTACGTT
ACGGTGATATTTTCCTAGAGAAAGCATTACCACGTGGCGGTTGGATGGAACTTGAGCTTAAAGAAGTAAACTACTTGCGT
GATATGGTTGAATTACCACACGAAAAAGAAAGTTTATTAGATGTAGATAAAGAATCACGTAAGCGTGCTAAATCTAAATC
TCAAAAGATCCGCCGTGCAGTTAAGCGTCATGATGAGCGTACAACGGAAGATCGTAATACCGCTGGTGGTCGTCGTGGTC
GTAATAATAAAAAACCAGTCGGTGGTTCATTGCCAACAGCGAAGAAACCGACAAGCATTGAAAGTGCAAAACCTGCCGGT
AAAGGCAAGCCAAAGGGCAAACCTTCAGCTAATGGTGCAAAACCGATCAGAACTAAGTTTTCACCAAATGGTAAAGCACC
AAAATCAGCACCAAGACAGCGTTAA

Upstream 100 bases:

>100_bases
CATAGAATTTTATAGTGATTGAGGGATAGAGTGATTTTCACTTAGGCCCTTGATTAACAAGAAAATAATTGACGCCTGTG
AAGGCGACATAAGGTAAAAA

Downstream 100 bases:

>100_bases
TTAACGCTTTCTCGATGAATTAAAAAAAGGGTTGAAGCCACATGGTTTCAGCCCTTTTTACTGTCTGGTGTAATCTATTA
TTGTTAGTACATCACACCAA

Product: ribosomal large subunit pseudouridine synthase B (rRNA pseudouridylate synthase B)

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSEKLQKVLARAGLGSRRELETMIQAGRVSIDGQKAKLGDRLENVDARIRIDGHVVSVKESTDVICRVLAYHKPEGELCT
RHDPEGRRTVFDRLPKIKDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRIFGDINDKMVKNLVTGVK
LEDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
DMVELPHEKESLLDVDKESRKRAKSKSQKIRRAVKRHDERTTEDRNTAGGRRGRNNKKPVGGSLPTAKKPTSIESAKPAG
KGKPKGKPSANGAKPIRTKFSPNGKAPKSAPRQR

Sequences:

>Translated_354_residues
MSEKLQKVLARAGLGSRRELETMIQAGRVSIDGQKAKLGDRLENVDARIRIDGHVVSVKESTDVICRVLAYHKPEGELCT
RHDPEGRRTVFDRLPKIKDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRIFGDINDKMVKNLVTGVK
LEDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
DMVELPHEKESLLDVDKESRKRAKSKSQKIRRAVKRHDERTTEDRNTAGGRRGRNNKKPVGGSLPTAKKPTSIESAKPAG
KGKPKGKPSANGAKPIRTKFSPNGKAPKSAPRQR
>Mature_353_residues
SEKLQKVLARAGLGSRRELETMIQAGRVSIDGQKAKLGDRLENVDARIRIDGHVVSVKESTDVICRVLAYHKPEGELCTR
HDPEGRRTVFDRLPKIKDSRWVSVGRLDANTAGLLLFTTDGELANRLMHPSRKVEREYMCRIFGDINDKMVKNLVTGVKL
EDGEARFEDVMYAGGEGMNHTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLRD
MVELPHEKESLLDVDKESRKRAKSKSQKIRRAVKRHDERTTEDRNTAGGRRGRNNKKPVGGSLPTAKKPTSIESAKPAGK
GKPKGKPSANGAKPIRTKFSPNGKAPKSAPRQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=299, Percent_Identity=63.8795986622074, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790453, Length=252, Percent_Identity=26.984126984127, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=28.494623655914, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 39943; Mature: 39812

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGLGSRRELETMIQAGRVSIDGQKAKLGDRLENVDARIRIDGHVVSVKE
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHCCCEEEEECCEEEEECC
STDVICRVLAYHKPEGELCTRHDPEGRRTVFDRLPKIKDSRWVSVGRLDANTAGLLLFTT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEC
DGELANRLMHPSRKVEREYMCRIFGDINDKMVKNLVTGVKLEDGEARFEDVMYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
EEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
DMVELPHEKESLLDVDKESRKRAKSKSQKIRRAVKRHDERTTEDRNTAGGRRGRNNKKPV
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GGSLPTAKKPTSIESAKPAGKGKPKGKPSANGAKPIRTKFSPNGKAPKSAPRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGLGSRRELETMIQAGRVSIDGQKAKLGDRLENVDARIRIDGHVVSVKE
CHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHHCCCEEEEECCEEEEECC
STDVICRVLAYHKPEGELCTRHDPEGRRTVFDRLPKIKDSRWVSVGRLDANTAGLLLFTT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEC
DGELANRLMHPSRKVEREYMCRIFGDINDKMVKNLVTGVKLEDGEARFEDVMYAGGEGMN
CHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCHHHHHHHHCCCCCCC
HTFYVVINEGRNREVRRLWDSQGVTVSRLKRVRYGDIFLEKALPRGGWMELELKEVNYLR
EEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
DMVELPHEKESLLDVDKESRKRAKSKSQKIRRAVKRHDERTTEDRNTAGGRRGRNNKKPV
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GGSLPTAKKPTSIESAKPAGKGKPKGKPSANGAKPIRTKFSPNGKAPKSAPRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]