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The map label for this gene is hisF [H]

Identifier: 209694689

GI number: 209694689

Start: 1242338

End: 1243111

Strand: Direct

Name: hisF [H]

Synonym: VSAL_I1139

Alternate gene names: 209694689

Gene position: 1242338-1243111 (Clockwise)

Preceding gene: 209694688

Following gene: 209694690

Centisome position: 37.36

GC content: 42.51

Gene sequence:

>774_bases
ATGTTGGCAAAGCGAATAATCCCTTGTTTAGATGTTAAAGATGGACAAGTCGTAAAAGGCGTTCAATTTCGTAATCACGA
GATCATTGGTGATATTGTTCCTCTAGCGCAGCGTTATGCAGAAGAGGGCGCAGATGAATTGGTCTTTTATGATATCACCG
CATCTAGTGATGGTCGCGTTGTAGATAAAAGCTGGGTCGCACGTGTAGCTGAAGTGATTGATATTCCATTCTGTGTGGCG
GGTGGCATTAAAACAGCAGAAGATGCCGCGAAGATTTTAGAGTTTGGTGCTGATAAAGTATCGATTAACTCCCCTGCACT
GGCTAACCCTGAACTCATCACTGAGCTTGCGGATAAATTTGGCGTTCAATGTATCGTTGTTGGTATTGATTCATACTTTG
ATAAAGAAACAGGTAAGTACCAAGTTTACCAATTCACGGGTGATGAAGAACGCACCAAAGCAACCAAATGGGAAACCAAA
GATTGGGTGCAAGAAGTACAAAAACGCGGTGCGGGTGAAATCGTGCTTAATATGATGAATCAAGATGGTGTGCGTAACGG
TTACGATCTTGAACAATTAAACATGGTACGTGAAGTGTGTCATGTTCCGCTTATCGCTTCAGGCGGCGCGGGTGAAATGG
TGCACTTTGCCGATGTATACAAGAAAACAAACGTGGACGGTGCTTTAGCAGCATCGGTATTCCATAAGCAAATCATCAAT
ATTGGTGAATTAAAAGAATATTTAGCACAACAAAAAATAGAGGTACGCCGATGA

Upstream 100 bases:

>100_bases
CTCTTGATGATATCGTCGCGCTTAAAGGCTCAGGTGTTGCTGGAGTCATCGTTGGACGAGCACTGCTTGATGGCAAGTTC
ACCGCAGAGGAGGCATTCCA

Downstream 100 bases:

>100_bases
GCCAACAAGATTTTGCAACACGTATTGATTGGGAAAAAGTGGACGGCTTAGTACCTGCAATTATTCAAGACTTTAGTTCA
AGCCAAGTATTGATGATGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCHVPLIASGGAGEMVHFADVYKKTNVDGALAASVFHKQIIN
IGELKEYLAQQKIEVRR

Sequences:

>Translated_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCHVPLIASGGAGEMVHFADVYKKTNVDGALAASVFHKQIIN
IGELKEYLAQQKIEVRR
>Mature_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKETGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCHVPLIASGGAGEMVHFADVYKKTNVDGALAASVFHKQIIN
IGELKEYLAQQKIEVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=84.375, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=31.6455696202532, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28497; Mature: 28497

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKTAEDAAKILEFGADKVSINSPALANPELITELADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDSYFDKETGKYQVYQFTGDEERTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHHCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLNMVREVCHVPLIASGGAGEMVHFADVYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKEYLAQQKIEVRR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKTAEDAAKILEFGADKVSINSPALANPELITELADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDSYFDKETGKYQVYQFTGDEERTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHHCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLNMVREVCHVPLIASGGAGEMVHFADVYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKEYLAQQKIEVRR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA