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The map label for this gene is hisA

Identifier: 209694688

GI number: 209694688

Start: 1241619

End: 1242356

Strand: Direct

Name: hisA

Synonym: VSAL_I1138

Alternate gene names: 209694688

Gene position: 1241619-1242356 (Clockwise)

Preceding gene: 209694687

Following gene: 209694689

Centisome position: 37.34

GC content: 44.85

Gene sequence:

>738_bases
ATGATCATTCCAGCATTAGATTTAATTGAAGGCCAAGTAGTTCGTCTTTTCCAAGGCGACTACGGGCAAGTAACAGAATA
CAAAGTTGATCCGGCTGAACAGTTCAATCTGTATCACCAAGCGGGTGCTAATTGGCTGCACCTTGTTGATTTAACTGGCG
CAAAAGACACTACCGCACGTCAATTGGATTTGATCGCTCGTTTGCTAGCAAGCACGCCTGCCAATATTCAAATCGGTGGT
GGTGTACGTAATGAAGCGGATGTACAAGATTTATTGAACGCCGGCGCACAACGTGTGGTGATTGGTTCTACTGCGGTAAA
ACAGCCAGAGCTTGTTAAAGGTTGGATGGAAAAATACGGCGCAGAAAAAATCGTATTAGCGCTAGATATTAACATTGATG
AAAACGGCACACGTAATGTTGCTATTTCAGGTTGGCAAGAAGATTCAGGTGTAACTATTGAAGCACTGCTTGAAGATTAT
CTAACCGTTGGCCTTAAGCACGTCTTATGTACTGATATTTCTCGTGACGGCACACTGCAAGGTTCAAACGTAGAGCTGTA
CGTAGACTTATGTAAGCAGTACCCACAAGTGCAATTCCAATCTTCAGGTGGCATTGGTTCTCTTGATGATATCGTCGCGC
TTAAAGGCTCAGGTGTTGCTGGAGTCATCGTTGGACGAGCACTGCTTGATGGCAAGTTCACCGCAGAGGAGGCATTCCAA
TGTTGGCAAAGCGAATAA

Upstream 100 bases:

>100_bases
TGCGAGGTCGCTTCGCTTCGAGGTTTTAGGGCTCTTACTCGTCCCTCGGATCTCGTAATGAGCGTAGCGAATGTTCTCGA
TTCTGCTAATAAGGATATTT

Downstream 100 bases:

>100_bases
TCCCTTGTTTAGATGTTAAAGATGGACAAGTCGTAAAAGGCGTTCAATTTCGTAATCACGAGATCATTGGTGATATTGTT
CCTCTAGCGCAGCGTTATGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIPALDLIEGQVVRLFQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTARQLDLIARLLASTPANIQIGG
GVRNEADVQDLLNAGAQRVVIGSTAVKQPELVKGWMEKYGAEKIVLALDINIDENGTRNVAISGWQEDSGVTIEALLEDY
LTVGLKHVLCTDISRDGTLQGSNVELYVDLCKQYPQVQFQSSGGIGSLDDIVALKGSGVAGVIVGRALLDGKFTAEEAFQ
CWQSE

Sequences:

>Translated_245_residues
MIIPALDLIEGQVVRLFQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTARQLDLIARLLASTPANIQIGG
GVRNEADVQDLLNAGAQRVVIGSTAVKQPELVKGWMEKYGAEKIVLALDINIDENGTRNVAISGWQEDSGVTIEALLEDY
LTVGLKHVLCTDISRDGTLQGSNVELYVDLCKQYPQVQFQSSGGIGSLDDIVALKGSGVAGVIVGRALLDGKFTAEEAFQ
CWQSE
>Mature_245_residues
MIIPALDLIEGQVVRLFQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTARQLDLIARLLASTPANIQIGG
GVRNEADVQDLLNAGAQRVVIGSTAVKQPELVKGWMEKYGAEKIVLALDINIDENGTRNVAISGWQEDSGVTIEALLEDY
LTVGLKHVLCTDISRDGTLQGSNVELYVDLCKQYPQVQFQSSGGIGSLDDIVALKGSGVAGVIVGRALLDGKFTAEEAFQ
CWQSE

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=63.9344262295082, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ALISL (B6EJ92)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262616.1
- ProteinModelPortal:   B6EJ92
- GeneID:   6988666
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1138
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26460; Mature: 26460

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: ACT_SITE 7-7 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPALDLIEGQVVRLFQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTAR
CCCCCHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLDLIARLLASTPANIQIGGGVRNEADVQDLLNAGAQRVVIGSTAVKQPELVKGWMEKYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCC
AEKIVLALDINIDENGTRNVAISGWQEDSGVTIEALLEDYLTVGLKHVLCTDISRDGTLQ
CCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVELYVDLCKQYPQVQFQSSGGIGSLDDIVALKGSGVAGVIVGRALLDGKFTAEEAFQ
CCCEEEEEHHHHHCCCEEEECCCCCCCHHHEEEECCCCEEHHHHHHHHHCCCCCHHHHHH
CWQSE
HHCCC
>Mature Secondary Structure
MIIPALDLIEGQVVRLFQGDYGQVTEYKVDPAEQFNLYHQAGANWLHLVDLTGAKDTTAR
CCCCCHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCHHH
QLDLIARLLASTPANIQIGGGVRNEADVQDLLNAGAQRVVIGSTAVKQPELVKGWMEKYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCC
AEKIVLALDINIDENGTRNVAISGWQEDSGVTIEALLEDYLTVGLKHVLCTDISRDGTLQ
CCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVELYVDLCKQYPQVQFQSSGGIGSLDDIVALKGSGVAGVIVGRALLDGKFTAEEAFQ
CCCEEEEEHHHHHCCCEEEECCCCCCCHHHEEEECCCCEEHHHHHHHHHCCCCCHHHHHH
CWQSE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA