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The map label for this gene is hisH [H]

Identifier: 209694687

GI number: 209694687

Start: 1240890

End: 1241504

Strand: Direct

Name: hisH [H]

Synonym: VSAL_I1137

Alternate gene names: 209694687

Gene position: 1240890-1241504 (Clockwise)

Preceding gene: 209694686

Following gene: 209694688

Centisome position: 37.32

GC content: 41.63

Gene sequence:

>615_bases
ATGACAAAGCAAAATGTCGTTATTATTGATACAGGCTGCTCTAACGTATCGTCAGTGAGATTTGCGATTGAGCGTTTGGG
TTATGACGTTACGATAAGCAGAGATCCACAAGTGGTATTAGCAGCAGATAAGCTGTTTTTACCGGGCGTTGGCACAGCAA
GCGAAGCGATGAAAAACTTGGCTGAACGAGATCTCATTGAATTGGTTAAACAAGTTGAGAAGCCACTGTTGGGTATCTGT
TTAGGTATGCAACTTTTGGGTAAGCTTTCTGAAGAGAAAGGCCAAAAAGCAGATCAAATTGTAGATTGTTTAGGCTTATG
TGACGGTGAAGTTCGTAAGCTTCAAACGGGTGATTTACCGCTGCCTCACATGGGTTGGAATACGATTAAGCCTATTGAAG
GTAATCCGCTGTTTAAAGACATTCCAGAGAACAGTTACTTTTACTTCGTACACAGCTTTGGCATGCAAGTGGGTGATTAT
ACCATTGCGACTTGTGACTATGGACAACCATTTACTGCGGCATTACAAGTGGGTAATTATTACGGTGTTCAGTTTCACCC
TGAGCGTTCAAGTAAAGTGGGTTCAACGCTTATTCAGAACTTTTTGGAGTTATAA

Upstream 100 bases:

>100_bases
TTGAAAGCTTATTTAAAGCCTTTGGCCGTACTTTACGCCAAGCAATTAAAGTGGAAGGTAATGAACTACCAAGCTCAAAA
GGCGTATTGTAGGAGTGACT

Downstream 100 bases:

>100_bases
GAGCAGGGACTAGGTGCGAGGTCGCTTCGCTTCGAGGTTTTAGGGCTCTTACTCGTCCCTCGGATCTCGTAATGAGCGTA
GCGAATGTTCTCGATTCTGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTKQNVVIIDTGCSNVSSVRFAIERLGYDVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGIC
LGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPIEGNPLFKDIPENSYFYFVHSFGMQVGDY
TIATCDYGQPFTAALQVGNYYGVQFHPERSSKVGSTLIQNFLEL

Sequences:

>Translated_204_residues
MTKQNVVIIDTGCSNVSSVRFAIERLGYDVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGIC
LGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPIEGNPLFKDIPENSYFYFVHSFGMQVGDY
TIATCDYGQPFTAALQVGNYYGVQFHPERSSKVGSTLIQNFLEL
>Mature_203_residues
TKQNVVIIDTGCSNVSSVRFAIERLGYDVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGICL
GMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPIEGNPLFKDIPENSYFYFVHSFGMQVGDYT
IATCDYGQPFTAALQVGNYYGVQFHPERSSKVGSTLIQNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=58.5, Blast_Score=245, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=32.7102803738318, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22491; Mature: 22360

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQNVVIIDTGCSNVSSVRFAIERLGYDVTISRDPQVVLAADKLFLPGVGTASEAMKNL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECEEECCCCCCHHHHHHHH
AERDLIELVKQVEKPLLGICLGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHEEECCCCC
LPHMGWNTIKPIEGNPLFKDIPENSYFYFVHSFGMQVGDYTIATCDYGQPFTAALQVGNY
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHEECCCE
YGVQFHPERSSKVGSTLIQNFLEL
ECEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKQNVVIIDTGCSNVSSVRFAIERLGYDVTISRDPQVVLAADKLFLPGVGTASEAMKNL
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECEEECCCCCCHHHHHHHH
AERDLIELVKQVEKPLLGICLGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHEEECCCCC
LPHMGWNTIKPIEGNPLFKDIPENSYFYFVHSFGMQVGDYTIATCDYGQPFTAALQVGNY
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHEECCCE
YGVQFHPERSSKVGSTLIQNFLEL
ECEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA