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The map label for this gene is dnaX [H]

Identifier: 209694644

GI number: 209694644

Start: 1180101

End: 1182245

Strand: Direct

Name: dnaX [H]

Synonym: VSAL_I1085

Alternate gene names: 209694644

Gene position: 1180101-1182245 (Clockwise)

Preceding gene: 209694643

Following gene: 209694645

Centisome position: 35.49

GC content: 41.96

Gene sequence:

>2145_bases
ATGAGCTATCAAGTTCTGGCACGTAAATGGCGACCACATCATTTTAAAGATGTTGTTGGTCAATCCCATGTGCTTACGGC
GTTGGAAAACGCGCTTGACCATAATCGACTTCATCACGCTTACTTATTTAGTGGCACTCGCGGTGTGGGTAAAACTACTA
TTGCTCGTTTGTTCGCTAAAGGACTAAACTGTGAAGAGGGGATTACATCAACACCTTGTGGCGTGTGTTCTACCTGTAAA
GAAATTGATGAAGGACGATTTGTCGATTTACTTGAGATTGATGCCGCTTCTAGAACCAAAGTAGAAGATACTCGTGATCT
ATTAGATAACGTTCAATATAAACCTGCGCGTGGTCGTTTTAAGGTTTATTTGATCGATGAAGTTCACATGCTATCGCGAC
ACAGTTTCAATGCATTATTAAAAACATTGGAAGAGCCGCCAGAATACGTCAAATTCATATTAGCCACGACCGATCCACAA
AAACTGCCTGTGACGATTTTATCTCGTTGCTTACAGTTTCATCTGAAACACATCAGTGTTGATCAAATTCATGCGCAATT
AGATCTCGTTTTAGACAAAGAAGAAGTGTCTAAAGATGGAAGAGCGTTAGGGCTGATAGCTCATGCGGCAGATGGCAGTA
TGCGTGATGCATTAAGTTTGACCGATCAAGCGATTGCGTTAGGTAATGGTAATGTAACTGCTGATATTGTGTCTATGATG
TTGGGTACGTTAGATACCGATCAAGCGATTCGTATTCTAGAAGCGATCAGCACAAAAAACATGCAAACGCTGATGAGCTG
TATCAATGATTTAGCAGATATTGGTGTTGAGTGGGATGGGTTAGCAAAAGAAATTGCGGCACAACTGCATAAATTGGCGA
TGTACCAAGCGTTACCTGCAACGTTAGATAAAACTCAACCGGATGCTGAGAGAATTGAATTATTGAGTGGTCAGTTATCG
CCACAAGAAGTTCAATTGTTCTACCAAATTGCATTGCAAAGCCGTAATGATTTACCTTTAGCCCCAACCGAAAAAACGGG
CGTAGAGATGATGTTATTGCGTATGGTCGCATTTCGTCCTGTGCATGCTCAAGCAATAGAGGCATTACCGATTGCCGTGC
CTAGCATGGAACCTGCTGCCGTAAATGTTGCACCTCAACAACCTGCGCCAGCCTCTAGAGTTCAGCAAGTTATTGCATCA
GCTCCAAATGCTGAAGTGCCTGTTGCTCCTAAGCCAATGCAACCGCAAGCAGTATCTCAAACGGTGTATTCTCCGGTTTC
TAATTCGCAACAATCGAGTTCAGCACCGTTAGATTCTACAGAGGCTCAACCGCCAGTATATGATGCACCACCAATGGGGG
AATCATCACAATCTGATTCCGCACCAGTGCAAAATATACCTCAAGAGCCTATAGCAACCCCAAGAAGTGGGTTGTTAGGT
GTGCGTAATCAATTACGTAGTGCAAAAAATAATTTATCAAATACAACCACCGGTACTAGCCCAAAAAAGGGTAGTGCGGC
ATCCGATAAGAAAAAAACGAGCTCAGTGTTAGAACGTATTGCTGAAAAGCAACATCTCGCTCCTCGTACTCAGGTGTCGC
AACTTGGAACGTCTGTTTCTCAAGAGAAAAAGCCAGAAGAACCATACCGTTGGAAAGCCAGCCAGCCTGAAGTGAAAAAA
GTAGTGAGTGATATTACTCCTACGAAATTAAAGCAGGCACTTGAACATGAAAAAACACCTGAGATGGTTCAAGAACTTGT
TGATGAAACAGTAAAGCAAGATACGTGGTCTGCATTAGCGATGTCATTAGCGTTGCCTAAATTGGTACAACAACTTGTAT
TGAATTCTTCTTTTGAAGAACATGACGATCAGGTTTCATTGCATTTACGTTCTGCACAATCACATTTAATTAATGACAAG
GCGCATGAATTGGTTAGCCAAGCGTTAAGCTCTGCTCTAAACAAAGAGATTAAATTAAACATTGATATTAGTGCAGAAGG
TACAAGCCCGTTAGAGTTACGTGAGCAAATGTATCAAACTAAATTACAGACGGCATACCAGAGCTTGCAACAAGATCCTC
ATGTTAATTTCTTTATGCAGCGTTACGGTGCAGAAATAGATCAAGAAAGCGTGAGACCAATTTAA

Upstream 100 bases:

>100_bases
TTCATATACACAATAAATGTAATGAGATCTCTCTATCAATGAAGATCTCATTTTTTTGCTGTGCTACGATTTAGATCTAT
TTTATTTTGGGCTGTATTCT

Downstream 100 bases:

>100_bases
AGAAAGATAAGGTAAGAATGGGTTGAAATTCTACATATCATCCCCATTTTTGTAGTATTAAAGATATAAACACTAAGAGA
GAATGAATATGTTCGGTGGA

Product: DNA polymerase III subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSYQVLARKWRPHHFKDVVGQSHVLTALENALDHNRLHHAYLFSGTRGVGKTTIARLFAKGLNCEEGITSTPCGVCSTCK
EIDEGRFVDLLEIDAASRTKVEDTRDLLDNVQYKPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFILATTDPQ
KLPVTILSRCLQFHLKHISVDQIHAQLDLVLDKEEVSKDGRALGLIAHAADGSMRDALSLTDQAIALGNGNVTADIVSMM
LGTLDTDQAIRILEAISTKNMQTLMSCINDLADIGVEWDGLAKEIAAQLHKLAMYQALPATLDKTQPDAERIELLSGQLS
PQEVQLFYQIALQSRNDLPLAPTEKTGVEMMLLRMVAFRPVHAQAIEALPIAVPSMEPAAVNVAPQQPAPASRVQQVIAS
APNAEVPVAPKPMQPQAVSQTVYSPVSNSQQSSSAPLDSTEAQPPVYDAPPMGESSQSDSAPVQNIPQEPIATPRSGLLG
VRNQLRSAKNNLSNTTTGTSPKKGSAASDKKKTSSVLERIAEKQHLAPRTQVSQLGTSVSQEKKPEEPYRWKASQPEVKK
VVSDITPTKLKQALEHEKTPEMVQELVDETVKQDTWSALAMSLALPKLVQQLVLNSSFEEHDDQVSLHLRSAQSHLINDK
AHELVSQALSSALNKEIKLNIDISAEGTSPLELREQMYQTKLQTAYQSLQQDPHVNFFMQRYGAEIDQESVRPI

Sequences:

>Translated_714_residues
MSYQVLARKWRPHHFKDVVGQSHVLTALENALDHNRLHHAYLFSGTRGVGKTTIARLFAKGLNCEEGITSTPCGVCSTCK
EIDEGRFVDLLEIDAASRTKVEDTRDLLDNVQYKPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFILATTDPQ
KLPVTILSRCLQFHLKHISVDQIHAQLDLVLDKEEVSKDGRALGLIAHAADGSMRDALSLTDQAIALGNGNVTADIVSMM
LGTLDTDQAIRILEAISTKNMQTLMSCINDLADIGVEWDGLAKEIAAQLHKLAMYQALPATLDKTQPDAERIELLSGQLS
PQEVQLFYQIALQSRNDLPLAPTEKTGVEMMLLRMVAFRPVHAQAIEALPIAVPSMEPAAVNVAPQQPAPASRVQQVIAS
APNAEVPVAPKPMQPQAVSQTVYSPVSNSQQSSSAPLDSTEAQPPVYDAPPMGESSQSDSAPVQNIPQEPIATPRSGLLG
VRNQLRSAKNNLSNTTTGTSPKKGSAASDKKKTSSVLERIAEKQHLAPRTQVSQLGTSVSQEKKPEEPYRWKASQPEVKK
VVSDITPTKLKQALEHEKTPEMVQELVDETVKQDTWSALAMSLALPKLVQQLVLNSSFEEHDDQVSLHLRSAQSHLINDK
AHELVSQALSSALNKEIKLNIDISAEGTSPLELREQMYQTKLQTAYQSLQQDPHVNFFMQRYGAEIDQESVRPI
>Mature_713_residues
SYQVLARKWRPHHFKDVVGQSHVLTALENALDHNRLHHAYLFSGTRGVGKTTIARLFAKGLNCEEGITSTPCGVCSTCKE
IDEGRFVDLLEIDAASRTKVEDTRDLLDNVQYKPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFILATTDPQK
LPVTILSRCLQFHLKHISVDQIHAQLDLVLDKEEVSKDGRALGLIAHAADGSMRDALSLTDQAIALGNGNVTADIVSMML
GTLDTDQAIRILEAISTKNMQTLMSCINDLADIGVEWDGLAKEIAAQLHKLAMYQALPATLDKTQPDAERIELLSGQLSP
QEVQLFYQIALQSRNDLPLAPTEKTGVEMMLLRMVAFRPVHAQAIEALPIAVPSMEPAAVNVAPQQPAPASRVQQVIASA
PNAEVPVAPKPMQPQAVSQTVYSPVSNSQQSSSAPLDSTEAQPPVYDAPPMGESSQSDSAPVQNIPQEPIATPRSGLLGV
RNQLRSAKNNLSNTTTGTSPKKGSAASDKKKTSSVLERIAEKQHLAPRTQVSQLGTSVSQEKKPEEPYRWKASQPEVKKV
VSDITPTKLKQALEHEKTPEMVQELVDETVKQDTWSALAMSLALPKLVQQLVLNSSFEEHDDQVSLHLRSAQSHLINDKA
HELVSQALSSALNKEIKLNIDISAEGTSPLELREQMYQTKLQTAYQSLQQDPHVNFFMQRYGAEIDQESVRPI

Specific function: The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506491, Length=302, Percent_Identity=22.5165562913907, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI31881687, Length=302, Percent_Identity=22.5165562913907, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1786676, Length=361, Percent_Identity=66.2049861495845, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1787119, Length=231, Percent_Identity=29.004329004329, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322528, Length=308, Percent_Identity=23.0519480519481, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR001270
- InterPro:   IPR008921
- InterPro:   IPR022001
- InterPro:   IPR022754
- InterPro:   IPR012763
- InterPro:   IPR021029 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; PF12168 DNA_pol3_tau_4; PF12170 DNA_pol3_tau_5 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 78588; Mature: 78457

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQVLARKWRPHHFKDVVGQSHVLTALENALDHNRLHHAYLFSGTRGVGKTTIARLFAK
CCHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
GLNCEEGITSTPCGVCSTCKEIDEGRFVDLLEIDAASRTKVEDTRDLLDNVQYKPARGRF
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFILATTDPQKLPVTILSRCLQFHLKHISV
EEEEECHHHHHHHHHHHHHHHHHHCCHHHHEEEHCCCCCCCCCHHHHHHHHHHHHHHCCH
DQIHAQLDLVLDKEEVSKDGRALGLIAHAADGSMRDALSLTDQAIALGNGNVTADIVSMM
HHHHHHHHHEECHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHH
LGTLDTDQAIRILEAISTKNMQTLMSCINDLADIGVEWDGLAKEIAAQLHKLAMYQALPA
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLDKTQPDAERIELLSGQLSPQEVQLFYQIALQSRNDLPLAPTEKTGVEMMLLRMVAFRP
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
VHAQAIEALPIAVPSMEPAAVNVAPQQPAPASRVQQVIASAPNAEVPVAPKPMQPQAVSQ
HHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
TVYSPVSNSQQSSSAPLDSTEAQPPVYDAPPMGESSQSDSAPVQNIPQEPIATPRSGLLG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHH
VRNQLRSAKNNLSNTTTGTSPKKGSAASDKKKTSSVLERIAEKQHLAPRTQVSQLGTSVS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QEKKPEEPYRWKASQPEVKKVVSDITPTKLKQALEHEKTPEMVQELVDETVKQDTWSALA
HHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MSLALPKLVQQLVLNSSFEEHDDQVSLHLRSAQSHLINDKAHELVSQALSSALNKEIKLN
HHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEE
IDISAEGTSPLELREQMYQTKLQTAYQSLQQDPHVNFFMQRYGAEIDQESVRPI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SYQVLARKWRPHHFKDVVGQSHVLTALENALDHNRLHHAYLFSGTRGVGKTTIARLFAK
CHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
GLNCEEGITSTPCGVCSTCKEIDEGRFVDLLEIDAASRTKVEDTRDLLDNVQYKPARGRF
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCE
KVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFILATTDPQKLPVTILSRCLQFHLKHISV
EEEEECHHHHHHHHHHHHHHHHHHCCHHHHEEEHCCCCCCCCCHHHHHHHHHHHHHHCCH
DQIHAQLDLVLDKEEVSKDGRALGLIAHAADGSMRDALSLTDQAIALGNGNVTADIVSMM
HHHHHHHHHEECHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHHHH
LGTLDTDQAIRILEAISTKNMQTLMSCINDLADIGVEWDGLAKEIAAQLHKLAMYQALPA
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLDKTQPDAERIELLSGQLSPQEVQLFYQIALQSRNDLPLAPTEKTGVEMMLLRMVAFRP
HHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
VHAQAIEALPIAVPSMEPAAVNVAPQQPAPASRVQQVIASAPNAEVPVAPKPMQPQAVSQ
HHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
TVYSPVSNSQQSSSAPLDSTEAQPPVYDAPPMGESSQSDSAPVQNIPQEPIATPRSGLLG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHH
VRNQLRSAKNNLSNTTTGTSPKKGSAASDKKKTSSVLERIAEKQHLAPRTQVSQLGTSVS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QEKKPEEPYRWKASQPEVKKVVSDITPTKLKQALEHEKTPEMVQELVDETVKQDTWSALA
HHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MSLALPKLVQQLVLNSSFEEHDDQVSLHLRSAQSHLINDKAHELVSQALSSALNKEIKLN
HHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEE
IDISAEGTSPLELREQMYQTKLQTAYQSLQQDPHVNFFMQRYGAEIDQESVRPI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534795; 3018672; 9278503; 2186364; 2187190; 2181440; 1575709 [H]