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The map label for this gene is aphB [H]

Identifier: 209694640

GI number: 209694640

Start: 1176715

End: 1177590

Strand: Reverse

Name: aphB [H]

Synonym: VSAL_I1081

Alternate gene names: 209694640

Gene position: 1177590-1176715 (Counterclockwise)

Preceding gene: 209694647

Following gene: 209694629

Centisome position: 35.41

GC content: 38.36

Gene sequence:

>876_bases
ATGAAGTTAGATGATTTAAATTTGTTTCGACAAGTAGTGGATAATGGCAGCTATACTGCAGCATCAAGAAAGACTCTAAT
CCCTGTTGCAACAATTACTCGTCGCATTCAAGCCTTAGAAGATTCCTTAGATATTCGCCTTCTCAACCGCCATGCAAGAA
AACTGTCTCTCACCGAATCTGGTCAAAAATTTTATGACGAATGTTCTCCAATTCTGTCTAACTTAGTTAATATCAGTGAA
TATTTGAGTGAAGAGTGTCGTGGTGCCGCTGGGAAACTTAATATTGCGGCTCCCTCTAATTTAACAAAACGCATGATACA
GCCTATTTTAAATCAATTTATGCTGATTTATCCTAATATTAATATCAACCTAACCATGAGCAACCAACCTGAGCAGCTAG
ACCCAACGGATTGGGACATTATTTTCAGAGTTGGAAAACAACGTGACTCATCTCTTATTGCTCGCCAAATTGGTTCATTA
GAAGATATTTTAGTCGCTAGCCCTATTTACTTAGAGCAACGTTCTGAACCGACTCATGCGCAAGAATTACATGACCACAG
CTTATTGAAAGGCCATCCATTAATGCGCTGGCGCTTAACTAACATTGAGGGTGAATCTGTAACGATTACTGATGCGGGTC
GTTTTGAAGCCAGTGAGCTTAATGTTATTCGCATGGCTTGTTCTGATGGCTTAGGCATTACGTTAATGCCTGATATTATG
ATCAGTAAGTACCTTGAAGACGGTTCATTAGTTCGCGTATTAAAAGATTGGTCAGCCAACCCTAGAGACATTTACATGAT
CTATAACTACAAAGATCATCAGCCTGAAAAAGTACGATTATTCATTGATTTTGTTAGTAATCATATTCCAAAATAA

Upstream 100 bases:

>100_bases
TTTACTGATAGATTTCCACTTTAGTTGACTTATAACAACACCTACTTTAGTGTTTTAGTTACAATTCTACATTCGCTAAA
CCAGAGAGAATCTCAATATC

Downstream 100 bases:

>100_bases
CAAAAAAGCCTGTGAATACATCACAGGCTTTTTTAACTTTATAGGCGTAACGTAATTAAAATACGCTCATTGCTAAACGA
ATTTAAACGCCTTCATTGTA

Product: transcriptional regulator AphB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKLDDLNLFRQVVDNGSYTAASRKTLIPVATITRRIQALEDSLDIRLLNRHARKLSLTESGQKFYDECSPILSNLVNISE
YLSEECRGAAGKLNIAAPSNLTKRMIQPILNQFMLIYPNININLTMSNQPEQLDPTDWDIIFRVGKQRDSSLIARQIGSL
EDILVASPIYLEQRSEPTHAQELHDHSLLKGHPLMRWRLTNIEGESVTITDAGRFEASELNVIRMACSDGLGITLMPDIM
ISKYLEDGSLVRVLKDWSANPRDIYMIYNYKDHQPEKVRLFIDFVSNHIPK

Sequences:

>Translated_291_residues
MKLDDLNLFRQVVDNGSYTAASRKTLIPVATITRRIQALEDSLDIRLLNRHARKLSLTESGQKFYDECSPILSNLVNISE
YLSEECRGAAGKLNIAAPSNLTKRMIQPILNQFMLIYPNININLTMSNQPEQLDPTDWDIIFRVGKQRDSSLIARQIGSL
EDILVASPIYLEQRSEPTHAQELHDHSLLKGHPLMRWRLTNIEGESVTITDAGRFEASELNVIRMACSDGLGITLMPDIM
ISKYLEDGSLVRVLKDWSANPRDIYMIYNYKDHQPEKVRLFIDFVSNHIPK
>Mature_291_residues
MKLDDLNLFRQVVDNGSYTAASRKTLIPVATITRRIQALEDSLDIRLLNRHARKLSLTESGQKFYDECSPILSNLVNISE
YLSEECRGAAGKLNIAAPSNLTKRMIQPILNQFMLIYPNININLTMSNQPEQLDPTDWDIIFRVGKQRDSSLIARQIGSL
EDILVASPIYLEQRSEPTHAQELHDHSLLKGHPLMRWRLTNIEGESVTITDAGRFEASELNVIRMACSDGLGITLMPDIM
ISKYLEDGSLVRVLKDWSANPRDIYMIYNYKDHQPEKVRLFIDFVSNHIPK

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=28.6206896551724, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=26.8965517241379, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=25.938566552901, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=26.9230769230769, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=26.8965517241379, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=25.6944444444444, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=25.5972696245734, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789173, Length=285, Percent_Identity=22.4561403508772, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082132, Length=263, Percent_Identity=24.3346007604563, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=124, Percent_Identity=25, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33195; Mature: 33195

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDDLNLFRQVVDNGSYTAASRKTLIPVATITRRIQALEDSLDIRLLNRHARKLSLTES
CCCCHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHH
GQKFYDECSPILSNLVNISEYLSEECRGAAGKLNIAAPSNLTKRMIQPILNQFMLIYPNI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCC
NINLTMSNQPEQLDPTDWDIIFRVGKQRDSSLIARQIGSLEDILVASPIYLEQRSEPTHA
EEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCEEECCCCCCHH
QELHDHSLLKGHPLMRWRLTNIEGESVTITDAGRFEASELNVIRMACSDGLGITLMPDIM
HHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEEEECCCCCCEEEEHHHH
ISKYLEDGSLVRVLKDWSANPRDIYMIYNYKDHQPEKVRLFIDFVSNHIPK
HHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEHEEHHHHCCCC
>Mature Secondary Structure
MKLDDLNLFRQVVDNGSYTAASRKTLIPVATITRRIQALEDSLDIRLLNRHARKLSLTES
CCCCHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHH
GQKFYDECSPILSNLVNISEYLSEECRGAAGKLNIAAPSNLTKRMIQPILNQFMLIYPNI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCC
NINLTMSNQPEQLDPTDWDIIFRVGKQRDSSLIARQIGSLEDILVASPIYLEQRSEPTHA
EEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCEEECCCCCCHH
QELHDHSLLKGHPLMRWRLTNIEGESVTITDAGRFEASELNVIRMACSDGLGITLMPDIM
HHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEEEECCCCCCEEEEHHHH
ISKYLEDGSLVRVLKDWSANPRDIYMIYNYKDHQPEKVRLFIDFVSNHIPK
HHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEHEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]