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The map label for this gene is mnmC [H]

Identifier: 209694629

GI number: 209694629

Start: 1160921

End: 1162963

Strand: Reverse

Name: mnmC [H]

Synonym: VSAL_I1070

Alternate gene names: 209694629

Gene position: 1162963-1160921 (Counterclockwise)

Preceding gene: 209694640

Following gene: 209694626

Centisome position: 34.97

GC content: 41.36

Gene sequence:

>2043_bases
ATGTCACAAAACCACGTTATACCAAAGAACAACATAACCAATGCTGTCTTAGAATGGAATGAGTCTGGAACACCAGTCTC
CAATGATTTTGATGATGTGTATTTCTCTAATGATAATGGGTTAGAAGAAACACGCTATGTCTTTTTACAACAAAATCATC
TCCTGCAAAGATGGCAAGAGTGTGACTTACGTCGTTTTGTTATTGGCGAAACGGGATTTGGGACGGGTTTAAACTTCCTT
GCCGTGTGGCAATGCTTTAAAGAATTTCGCGCCCAATACCCTGATGCACCACTTAAAGAACTTCATTTTGTTAGCTTTGA
AAAATTTCCAGTCACTAAATCTGATTTAGTTAAAGCACACCAAGCATGGCCAGAGTTGGCCGAATTCGCAGAGCAACTAC
ATAAGTATTACCCTGCCGCCGTTCCTGATTGCCATCGTTTAGTACTAGAAGAAGGCATGATAACACTCGATCTCTGGTTT
GGTGACATAAAAGATTGTATGCCTCAAATTTGGATGGATGATCACGGTATTATTGATGCATGGTTCTTAGATGGTTTTGC
GCCAAGTAAAAACCCTGAGATGTGGAACCAAAATCTCTTCGATAATATGGCAAACCTCGCGAAAGAACACTGTACTTGTG
CAACATTCACTTCTGCGGGCTTTGTTCGTCGAGGATTAATAGAAGCAGGCTTTGACATGAAAAAAGTCAAAGGATTCGCT
CATAAACGAGAGATGATCGCAGGCACATTAACGGAACGAAAAGCAAAAGCAAATCACGCAGTTTGGTATGCCCGAACGAC
TCCGAAAGAGGTTACTGATGTCGCTATTATCGGTGGAGGCGTTGCCAGTGCTGCACTAGCTATGACGCTGCTTCGTCGTG
GTGTAAAAGTGACGGTGTATTGCAAAGATGAAAAAGCAGCACAAGGTGCATCAGGCAATCAACAAGGCGCGGTTTACCCT
CTATTAAATGAGAAATATAATTCACTTTCTCGCTTTTTTGCTCCCGGGTTTATTTTTGCACGTCAATTCATTGAACAAGC
CGCTGAACAGGTCGAATTTGATCACGATTGGTGTGGCGTAACTCAACTAAAATGGGACGATAAATCAGAAAAAAAACTCA
ACAAGATGCTTACTGCCCATTTTCCAAATGAATTAGTCACAGAATTAAACAAAGAAGACACCAATCTACACGTTGGGTTA
CCTATTAACATGGAAAGTGTTCATTATCCTCTTGGTGGATGGCTTTGCCCAAAACAACTCACTCGTGGGTTATTTGATCA
TTTTGCTGCCAACCCATTATTTACCTTACACTGTGATTCAGAAGTAACCTCTCTTCAACAAAGCAATGATAAACAATGGC
ATGTGTTATTAGGCGATAGCGTACATCCACATCAAACCGTCATTGTCGCTAATGGGCATCGTTTCACTGATTTTGAACAA
ACGAAAGATATCCCTGCGACCGCAGTTCGTGGGCAAGTGAGTCATATTCCCACCACTGAATCATTGAAAAAACTCAACAC
CGTATTGTGCTATGACGGTTACTTGACACCAGAAAACACCAAATACCAAACTCATTGTATTGGAGCGAGTTACGATCGTC
GTGATTTAGATTTAAAATTTAAAGAATCAGACCAAGTTGAAAATGGTGAGCGTTTACATAAATGTATTCCTAACGAAGTA
TGGCCAAAAGAAGCAGATACAAGCTCAAACCAAGCTCGTGTTGGTATTCGCTGCGCCAGTCGTGACCACTTGCCCTTCAT
TGGTAATGTCGTTCGATTTGAAGCGATGCAGGAAGAATATAAGAACCTTTATAAAAAGCGTCATTGGCAGCGCGATGCAA
AAGAGATCCCTATCTATGAAGGTTTATTCTGTATGTTGACGCTGGGTTCTAGAGGCTTAAGCTCGGCTCCTTTATTAGCG
GAAACCTTAGTTTCTCAAATAATGGGCGATCCAATCCCATTGCCTAATTCAGTGCTTGAAGGTCTACATCCGGGAAGATT
ATGGGTAAGAAGGTTATTGAAAGGAAAACCGTTGGATATTTAA

Upstream 100 bases:

>100_bases
TAAATCTTGGTAGATAATAGCGAAGATAGCACAACAAAGTGGTCAGCTTTCCTATAAGTCGTTAAAATCCGACGTAGATT
ATCTTTAATGGTTCAAATTT

Downstream 100 bases:

>100_bases
GAGAAAGGAAGAGCCGATTTTAGCTCGGTCGCAAGCTCTCTTACCCGTTTCAGGGGAGTTCGTTGTAAATGATAAACTCA
TAACGAACTCCTCTGATCAC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MSQNHVIPKNNITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLLQRWQECDLRRFVIGETGFGTGLNFL
AVWQCFKEFRAQYPDAPLKELHFVSFEKFPVTKSDLVKAHQAWPELAEFAEQLHKYYPAAVPDCHRLVLEEGMITLDLWF
GDIKDCMPQIWMDDHGIIDAWFLDGFAPSKNPEMWNQNLFDNMANLAKEHCTCATFTSAGFVRRGLIEAGFDMKKVKGFA
HKREMIAGTLTERKAKANHAVWYARTTPKEVTDVAIIGGGVASAALAMTLLRRGVKVTVYCKDEKAAQGASGNQQGAVYP
LLNEKYNSLSRFFAPGFIFARQFIEQAAEQVEFDHDWCGVTQLKWDDKSEKKLNKMLTAHFPNELVTELNKEDTNLHVGL
PINMESVHYPLGGWLCPKQLTRGLFDHFAANPLFTLHCDSEVTSLQQSNDKQWHVLLGDSVHPHQTVIVANGHRFTDFEQ
TKDIPATAVRGQVSHIPTTESLKKLNTVLCYDGYLTPENTKYQTHCIGASYDRRDLDLKFKESDQVENGERLHKCIPNEV
WPKEADTSSNQARVGIRCASRDHLPFIGNVVRFEAMQEEYKNLYKKRHWQRDAKEIPIYEGLFCMLTLGSRGLSSAPLLA
ETLVSQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI

Sequences:

>Translated_680_residues
MSQNHVIPKNNITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLLQRWQECDLRRFVIGETGFGTGLNFL
AVWQCFKEFRAQYPDAPLKELHFVSFEKFPVTKSDLVKAHQAWPELAEFAEQLHKYYPAAVPDCHRLVLEEGMITLDLWF
GDIKDCMPQIWMDDHGIIDAWFLDGFAPSKNPEMWNQNLFDNMANLAKEHCTCATFTSAGFVRRGLIEAGFDMKKVKGFA
HKREMIAGTLTERKAKANHAVWYARTTPKEVTDVAIIGGGVASAALAMTLLRRGVKVTVYCKDEKAAQGASGNQQGAVYP
LLNEKYNSLSRFFAPGFIFARQFIEQAAEQVEFDHDWCGVTQLKWDDKSEKKLNKMLTAHFPNELVTELNKEDTNLHVGL
PINMESVHYPLGGWLCPKQLTRGLFDHFAANPLFTLHCDSEVTSLQQSNDKQWHVLLGDSVHPHQTVIVANGHRFTDFEQ
TKDIPATAVRGQVSHIPTTESLKKLNTVLCYDGYLTPENTKYQTHCIGASYDRRDLDLKFKESDQVENGERLHKCIPNEV
WPKEADTSSNQARVGIRCASRDHLPFIGNVVRFEAMQEEYKNLYKKRHWQRDAKEIPIYEGLFCMLTLGSRGLSSAPLLA
ETLVSQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI
>Mature_679_residues
SQNHVIPKNNITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLLQRWQECDLRRFVIGETGFGTGLNFLA
VWQCFKEFRAQYPDAPLKELHFVSFEKFPVTKSDLVKAHQAWPELAEFAEQLHKYYPAAVPDCHRLVLEEGMITLDLWFG
DIKDCMPQIWMDDHGIIDAWFLDGFAPSKNPEMWNQNLFDNMANLAKEHCTCATFTSAGFVRRGLIEAGFDMKKVKGFAH
KREMIAGTLTERKAKANHAVWYARTTPKEVTDVAIIGGGVASAALAMTLLRRGVKVTVYCKDEKAAQGASGNQQGAVYPL
LNEKYNSLSRFFAPGFIFARQFIEQAAEQVEFDHDWCGVTQLKWDDKSEKKLNKMLTAHFPNELVTELNKEDTNLHVGLP
INMESVHYPLGGWLCPKQLTRGLFDHFAANPLFTLHCDSEVTSLQQSNDKQWHVLLGDSVHPHQTVIVANGHRFTDFEQT
KDIPATAVRGQVSHIPTTESLKKLNTVLCYDGYLTPENTKYQTHCIGASYDRRDLDLKFKESDQVENGERLHKCIPNEVW
PKEADTSSNQARVGIRCASRDHLPFIGNVVRFEAMQEEYKNLYKKRHWQRDAKEIPIYEGLFCMLTLGSRGLSSAPLLAE
TLVSQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=51.9519519519519, Blast_Score=684, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 77347; Mature: 77216

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNHVIPKNNITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLLQRWQE
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCHHHEEEEHHHHHHHHHHH
CDLRRFVIGETGFGTGLNFLAVWQCFKEFRAQYPDAPLKELHFVSFEKFPVTKSDLVKAH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHH
QAWPELAEFAEQLHKYYPAAVPDCHRLVLEEGMITLDLWFGDIKDCMPQIWMDDHGIIDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCEEE
WFLDGFAPSKNPEMWNQNLFDNMANLAKEHCTCATFTSAGFVRRGLIEAGFDMKKVKGFA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHH
HKREMIAGTLTERKAKANHAVWYARTTPKEVTDVAIIGGGVASAALAMTLLRRGVKVTVY
HHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEEECCHHHHHHHHHHHHHCCCEEEEE
CKDEKAAQGASGNQQGAVYPLLNEKYNSLSRFFAPGFIFARQFIEQAAEQVEFDHDWCGV
ECCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQLKWDDKSEKKLNKMLTAHFPNELVTELNKEDTNLHVGLPINMESVHYPLGGWLCPKQL
EEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCEECHHHH
TRGLFDHFAANPLFTLHCDSEVTSLQQSNDKQWHVLLGDSVHPHQTVIVANGHRFTDFEQ
HHHHHHHHHCCCEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHH
TKDIPATAVRGQVSHIPTTESLKKLNTVLCYDGYLTPENTKYQTHCIGASYDRRDLDLKF
HCCCCHHHHCCCCCCCCCHHHHHHHCEEEEECCEECCCCCCEEEEEECCCCCCCCCCEEE
KESDQVENGERLHKCIPNEVWPKEADTSSNQARVGIRCASRDHLPFIGNVVRFEAMQEEY
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KNLYKKRHWQRDAKEIPIYEGLFCMLTLGSRGLSSAPLLAETLVSQIMGDPIPLPNSVLE
HHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHC
GLHPGRLWVRRLLKGKPLDI
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQNHVIPKNNITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLLQRWQE
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCHHHEEEEHHHHHHHHHHH
CDLRRFVIGETGFGTGLNFLAVWQCFKEFRAQYPDAPLKELHFVSFEKFPVTKSDLVKAH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHH
QAWPELAEFAEQLHKYYPAAVPDCHRLVLEEGMITLDLWFGDIKDCMPQIWMDDHGIIDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCCEEE
WFLDGFAPSKNPEMWNQNLFDNMANLAKEHCTCATFTSAGFVRRGLIEAGFDMKKVKGFA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHH
HKREMIAGTLTERKAKANHAVWYARTTPKEVTDVAIIGGGVASAALAMTLLRRGVKVTVY
HHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEEECCHHHHHHHHHHHHHCCCEEEEE
CKDEKAAQGASGNQQGAVYPLLNEKYNSLSRFFAPGFIFARQFIEQAAEQVEFDHDWCGV
ECCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQLKWDDKSEKKLNKMLTAHFPNELVTELNKEDTNLHVGLPINMESVHYPLGGWLCPKQL
EEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCEECHHHH
TRGLFDHFAANPLFTLHCDSEVTSLQQSNDKQWHVLLGDSVHPHQTVIVANGHRFTDFEQ
HHHHHHHHHCCCEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHH
TKDIPATAVRGQVSHIPTTESLKKLNTVLCYDGYLTPENTKYQTHCIGASYDRRDLDLKF
HCCCCHHHHCCCCCCCCCHHHHHHHCEEEEECCEECCCCCCEEEEEECCCCCCCCCCEEE
KESDQVENGERLHKCIPNEVWPKEADTSSNQARVGIRCASRDHLPFIGNVVRFEAMQEEY
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KNLYKKRHWQRDAKEIPIYEGLFCMLTLGSRGLSSAPLLAETLVSQIMGDPIPLPNSVLE
HHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHC
GLHPGRLWVRRLLKGKPLDI
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA