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The map label for this gene is ubiF [H]

Identifier: 209694560

GI number: 209694560

Start: 1101750

End: 1102907

Strand: Direct

Name: ubiF [H]

Synonym: VSAL_I0997

Alternate gene names: 209694560

Gene position: 1101750-1102907 (Clockwise)

Preceding gene: 209694554

Following gene: 209694561

Centisome position: 33.13

GC content: 37.91

Gene sequence:

>1158_bases
ATGATGAAAGAATATGATTTCGCTATTGTTGGTGGTGGGATGGTTGGTGCCGCTGCCGCTATAGGTTTAGCTAAATTAGG
TTATTTAGTCGCTATTATTGAGCATAAAGAGCCAAGTACTTATGACAATAAACAGCCGATGGATATTCGAGTCTCTGCAA
TCTCTCAATCATCCGTTGATCTGCTTGAAAAACTAGGTGCATGGGAAAGTATTAAAGAAATGCGAGTTTGTCCTTATAAG
CGCTTAGAAACATGGGAACACCCTGAGTGTCGAACTCGCTTTCATTCTGATGCACTTAATATGGACCAGCTTGGCTTCAT
TGTTGAAAATCGTCTTATTCAATTAGGGTTATGGCAGACATTCTCTTGTTATGACAACCTCAACGTTATTACGCCTACAA
AAATAACCGATCTTGAGTTTTTTAGTGATCATAATGAACTGACGTTAGAAAATGGAGATAAAATTAAAGCGAAATGGGTT
CTTGGTGCTGATGGCGCTAATTCTTATGTTAGACAAAAAACTAACATAGGGATTACGGCTTGGGACTATCGTCAACATTG
CATGTTAATTAATATAGAAACAGAGCAACCACAACAAGATATTACGTGGCAATGGTTTACACCGTCAGGTCCTCGCTCAT
TTCTACCTTTACAGGGTAATAAAGGATCTTTGGTATGGTATGACTCACCAAAACGCATTAAACAGCTTTGCGCGATGAAC
TCGACTCAATTAGAAAGTGAAATAAAAGCATACTTTCCGAAAGAACTCGGAGAATTTACGGTAGAGCAATATGGTTCTTT
CCCTTTAACTCGTCGACATGCTCAAAAATACGTTGATCATCATTGTGTATTGCTTGGTGATGCCGCTCATACAATAAATC
CTCTAGCAGGACAGGGCGTTAATCTTGGATTTAAAGACATCAAAGTGCTATTAGAAGAAGTAGAGAAAAAAGGACTGAAT
ACAGATAGAGCTTTTAAAATGTATGAATCAAGACGAAAGATAGATAACTTAGCGATGCAAAGCAGTATGGATTTATTTTA
CACTTTATTTAGTAACTCTTTACCGCCGGTTAAGTTATTACGAAATATAGGGTTAAAATTAGCGGACTCGTCTGGGAAGT
TAAAAACACAAGCGCTGAAGTATGCGTTAGGTTTATAG

Upstream 100 bases:

>100_bases
AGACAACCACGAATCTTTTATGGGTATTATTTGAGAGATGTAGATCTGAAGTAAATCGCGGTAGAATAGTTAAAATTGTT
GGTTCATATAAGGTCTATGG

Downstream 100 bases:

>100_bases
AAATAATTCTAGGGACTAGAGCTTAGGGACGAGTAAAAGCGAATCTACAGAAAGTAAGGTGATCATTTGGTCACCTTTTT
TATTGCGTTAAAACCATTGA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MMKEYDFAIVGGGMVGAAAAIGLAKLGYLVAIIEHKEPSTYDNKQPMDIRVSAISQSSVDLLEKLGAWESIKEMRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWQTFSCYDNLNVITPTKITDLEFFSDHNELTLENGDKIKAKWV
LGADGANSYVRQKTNIGITAWDYRQHCMLINIETEQPQQDITWQWFTPSGPRSFLPLQGNKGSLVWYDSPKRIKQLCAMN
STQLESEIKAYFPKELGEFTVEQYGSFPLTRRHAQKYVDHHCVLLGDAAHTINPLAGQGVNLGFKDIKVLLEEVEKKGLN
TDRAFKMYESRRKIDNLAMQSSMDLFYTLFSNSLPPVKLLRNIGLKLADSSGKLKTQALKYALGL

Sequences:

>Translated_385_residues
MMKEYDFAIVGGGMVGAAAAIGLAKLGYLVAIIEHKEPSTYDNKQPMDIRVSAISQSSVDLLEKLGAWESIKEMRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWQTFSCYDNLNVITPTKITDLEFFSDHNELTLENGDKIKAKWV
LGADGANSYVRQKTNIGITAWDYRQHCMLINIETEQPQQDITWQWFTPSGPRSFLPLQGNKGSLVWYDSPKRIKQLCAMN
STQLESEIKAYFPKELGEFTVEQYGSFPLTRRHAQKYVDHHCVLLGDAAHTINPLAGQGVNLGFKDIKVLLEEVEKKGLN
TDRAFKMYESRRKIDNLAMQSSMDLFYTLFSNSLPPVKLLRNIGLKLADSSGKLKTQALKYALGL
>Mature_385_residues
MMKEYDFAIVGGGMVGAAAAIGLAKLGYLVAIIEHKEPSTYDNKQPMDIRVSAISQSSVDLLEKLGAWESIKEMRVCPYK
RLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWQTFSCYDNLNVITPTKITDLEFFSDHNELTLENGDKIKAKWV
LGADGANSYVRQKTNIGITAWDYRQHCMLINIETEQPQQDITWQWFTPSGPRSFLPLQGNKGSLVWYDSPKRIKQLCAMN
STQLESEIKAYFPKELGEFTVEQYGSFPLTRRHAQKYVDHHCVLLGDAAHTINPLAGQGVNLGFKDIKVLLEEVEKKGLN
TDRAFKMYESRRKIDNLAMQSSMDLFYTLFSNSLPPVKLLRNIGLKLADSSGKLKTQALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=397, Percent_Identity=30.4785894206549, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI32967307, Length=335, Percent_Identity=29.5522388059701, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=50.1298701298701, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=37.2448979591837, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789274, Length=366, Percent_Identity=25.9562841530055, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=30.3944315545244, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321694, Length=452, Percent_Identity=25.8849557522124, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19920760, Length=431, Percent_Identity=29.9303944315545, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43712; Mature: 43712

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKEYDFAIVGGGMVGAAAAIGLAKLGYLVAIIEHKEPSTYDNKQPMDIRVSAISQSSVD
CCCCCCEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCCHHHH
LLEKLGAWESIKEMRVCPYKRLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWQT
HHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCE
FSCYDNLNVITPTKITDLEFFSDHNELTLENGDKIKAKWVLGADGANSYVRQKTNIGITA
EHHHCCCCEECCCCEECCCEECCCCEEEECCCCEEEEEEEEECCCCCHHHHHHCCCCEEE
WDYRQHCMLINIETEQPQQDITWQWFTPSGPRSFLPLQGNKGSLVWYDSPKRIKQLCAMN
EECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCCEEEECCHHHHHHHHCCC
STQLESEIKAYFPKELGEFTVEQYGSFPLTRRHAQKYVDHHCVLLGDAAHTINPLAGQGV
CHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCHHHCCCCCCCCC
NLGFKDIKVLLEEVEKKGLNTDRAFKMYESRRKIDNLAMQSSMDLFYTLFSNSLPPVKLL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RNIGLKLADSSGKLKTQALKYALGL
HHCCCEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MMKEYDFAIVGGGMVGAAAAIGLAKLGYLVAIIEHKEPSTYDNKQPMDIRVSAISQSSVD
CCCCCCEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCCHHHH
LLEKLGAWESIKEMRVCPYKRLETWEHPECRTRFHSDALNMDQLGFIVENRLIQLGLWQT
HHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCE
FSCYDNLNVITPTKITDLEFFSDHNELTLENGDKIKAKWVLGADGANSYVRQKTNIGITA
EHHHCCCCEECCCCEECCCEECCCCEEEECCCCEEEEEEEEECCCCCHHHHHHCCCCEEE
WDYRQHCMLINIETEQPQQDITWQWFTPSGPRSFLPLQGNKGSLVWYDSPKRIKQLCAMN
EECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCCEEEECCHHHHHHHHCCC
STQLESEIKAYFPKELGEFTVEQYGSFPLTRRHAQKYVDHHCVLLGDAAHTINPLAGQGV
CHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCHHHCCCCCCCCC
NLGFKDIKVLLEEVEKKGLNTDRAFKMYESRRKIDNLAMQSSMDLFYTLFSNSLPPVKLL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RNIGLKLADSSGKLKTQALKYALGL
HHCCCEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]