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The map label for this gene is lipA

Identifier: 209694542

GI number: 209694542

Start: 1082414

End: 1083379

Strand: Reverse

Name: lipA

Synonym: VSAL_I0979

Alternate gene names: 209694542

Gene position: 1083379-1082414 (Counterclockwise)

Preceding gene: 209694543

Following gene: 209694536

Centisome position: 32.58

GC content: 42.75

Gene sequence:

>966_bases
ATGAGTAAGCCAATTCAAATGGAAAAAGGCGTTAAATACCGCGATGCCGATAAAATGGCATTAATTCCAGTTAAAAACAT
GCCAACAGAGAAAAAAGAAGTATTACGTAAGCCTGAGTGGATGAAGATCAAACTTCCATCAAGCAGTAAGCGTATTGATG
AAATTAAATCAGCAATGCGTAAAAATAATCTTCACTCTGTATGTGAAGAAGCATCTTGCCCTAACTTAGCAGAATGCTTT
AACCATGGTACGGCAACCTTCATGATCTTAGGTGCAATTTGTACCCGTCGCTGCCCTTTCTGTGATGTTGCTCATGGACG
CCCAGTCACTCCTGAAGCTGAAGAGCCGAAGAAACTGGCAAAAACGATTAAAGACATGAAACTGAAATATGTGGTAATCA
CATCAGTCGACCGTGATGATTTACGTGATGGCGGAGCACAACATTTTGCGGATTGTAATCGTGAAATTCGTGAATTAAAC
CCTGAGATCCGTATTGAAACGCTAGTTCCTGATTTTCGTGGTCGTATGGATCGCGCATTAGATGCGATGCATAGCAACCC
ACCAGATGTATTCAACCACAATTTAGAGACCGCTCCACGCCTATACCGTAAGGTTCGCCCTGGCGCAAATTACCAGTGGT
CACTTGACCTATTGAAGAAATTCAAAGAACAACACCCAACAGTACCAACCAAATCTGGTGTGATGATGGGTCTAGGCGAA
ACAAAAGAAGAAATAGTCGAAGTACTAAAAGACTTACGTGCTCACGGTGTAACGATGTTAACACTGGGTCAGTATTTAGC
ACCAAGTCGCCATCACCTACCCGTTGAGCGTTACGTTCCACCTGCAGAGTTTGATGAATTAAAAGTGATTGCTCTTGAAC
TTGGATTTACCCATGCGGCTTGTGGTCCATTTGTTCGCTCTTCTTACCATGCTGATCTTCAAGCGAAAGGCGAAGAAGTT
AAATAG

Upstream 100 bases:

>100_bases
AAAGCAGAACACGTTGAAGATGTTCACCCTTTACTTATTAAAGAATTAACAACATTACTTGATTACACTGATATTGAGTA
CATAACGGAAAGCAGCCATC

Downstream 100 bases:

>100_bases
GGTCTAGGGTCTAGGGTCTAGGGTCTAGGGTCTGAATAATAAAAAAGGTTGACGTTTTAGCGTCAACCTTTTTTGTTTTT
ACTAAACATTAATCAATATG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPTEKKEVLRKPEWMKIKLPSSSKRIDEIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRELN
PEIRIETLVPDFRGRMDRALDAMHSNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPTVPTKSGVMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKVIALELGFTHAACGPFVRSSYHADLQAKGEEV
K

Sequences:

>Translated_321_residues
MSKPIQMEKGVKYRDADKMALIPVKNMPTEKKEVLRKPEWMKIKLPSSSKRIDEIKSAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVTPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRELN
PEIRIETLVPDFRGRMDRALDAMHSNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPTVPTKSGVMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKVIALELGFTHAACGPFVRSSYHADLQAKGEEV
K
>Mature_320_residues
SKPIQMEKGVKYRDADKMALIPVKNMPTEKKEVLRKPEWMKIKLPSSSKRIDEIKSAMRKNNLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPVTPEAEEPKKLAKTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRELNP
EIRIETLVPDFRGRMDRALDAMHSNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPTVPTKSGVMMGLGET
KEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKVIALELGFTHAACGPFVRSSYHADLQAKGEEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=46.0750853242321, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=45.9143968871595, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=80.0623052959502, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.1602787456446, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.2343234323432, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=45.7746478873239, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ALISL (B6EIF3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262470.1
- GeneID:   6989011
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0979
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36438; Mature: 36306

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMEKGVKYRDADKMALIPVKNMPTEKKEVLRKPEWMKIKLPSSSKRIDEIKSAMR
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRELNPEIRIETLVPDFRGRMDRAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
DAMHSNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPTVPTKSGVMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKVIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCHHH
CGPFVRSSYHADLQAKGEEVK
HHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIQMEKGVKYRDADKMALIPVKNMPTEKKEVLRKPEWMKIKLPSSSKRIDEIKSAMR
CCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVTPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KTIKDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRELNPEIRIETLVPDFRGRMDRAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
DAMHSNPPDVFNHNLETAPRLYRKVRPGANYQWSLDLLKKFKEQHPTVPTKSGVMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKVIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCHHH
CGPFVRSSYHADLQAKGEEVK
HHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA