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The map label for this gene is lipB [H]

Identifier: 209694543

GI number: 209694543

Start: 1083372

End: 1084058

Strand: Reverse

Name: lipB [H]

Synonym: VSAL_I0980

Alternate gene names: 209694543

Gene position: 1084058-1083372 (Counterclockwise)

Preceding gene: 209694544

Following gene: 209694542

Centisome position: 32.6

GC content: 39.88

Gene sequence:

>687_bases
GTGATCCTATTGAACAAGCGTCTAGTAGTTAAAAACCTTGGCCGACAAGATTATGAGCCGGTATGGAAAGCGATGCATAC
CTTTACTGACGAACGCGATGAAAACTCGTGTGATGAAGTATGGTTAGTTGAGCACAATCCAGTATTCACTCAAGGACAAG
CAGGTAAAGAAGAACATGTTCTGGCTGCTGGAGATATCCCTGTCGTAAAAAGTGACCGTGGCGGGCAAGTAACCTACCAT
GGACCAGGTCAACTCGTCGCTTATGTTTTGATTAACTTACGCAGAAAGAAAATAGGTGTCCGTGAATTAGTCACACACAT
TGAAAACACGGTAATCAACACATTAAGTCAGTTTACTATTGAATCAGCAGCAAGACCTGATGCTCCGGGTGTGTATGTTG
ATAATAAAAAAATATGTTCTCTTGGTTTGCGTATCCGCAAAGGATGTTCATTCCACGGACTGGCACTAAACGTAAACATG
GATTTATCTCCTTTCCTAAGAATTAACCCTTGTGGTTATGCTGGAATGGAAATGATACAACTGCACGATTTAATTAAAGA
AAATGATACGGTAGAGAACAAAGCAGAACACGTTGAAGATGTTCACCCTTTACTTATTAAAGAATTAACAACATTACTTG
ATTACACTGATATTGAGTACATAACGGAAAGCAGCCATCATGAGTAA

Upstream 100 bases:

>100_bases
AATATTACCTGCCATTTTTAAATTTTGATATACCCCTACATTTCTTATCTATTCCTTCCTTTCTATCCTTGCTATCATTA
GCCAAATTTTCAAATCAAGT

Downstream 100 bases:

>100_bases
GCCAATTCAAATGGAAAAAGGCGTTAAATACCGCGATGCCGATAAAATGGCATTAATTCCAGTTAAAAACATGCCAACAG
AGAAAAAAGAAGTATTACGT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MILLNKRLVVKNLGRQDYEPVWKAMHTFTDERDENSCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPVVKSDRGGQVTYH
GPGQLVAYVLINLRRKKIGVRELVTHIENTVINTLSQFTIESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNVNM
DLSPFLRINPCGYAGMEMIQLHDLIKENDTVENKAEHVEDVHPLLIKELTTLLDYTDIEYITESSHHE

Sequences:

>Translated_228_residues
MILLNKRLVVKNLGRQDYEPVWKAMHTFTDERDENSCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPVVKSDRGGQVTYH
GPGQLVAYVLINLRRKKIGVRELVTHIENTVINTLSQFTIESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNVNM
DLSPFLRINPCGYAGMEMIQLHDLIKENDTVENKAEHVEDVHPLLIKELTTLLDYTDIEYITESSHHE
>Mature_228_residues
MILLNKRLVVKNLGRQDYEPVWKAMHTFTDERDENSCDEVWLVEHNPVFTQGQAGKEEHVLAAGDIPVVKSDRGGQVTYH
GPGQLVAYVLINLRRKKIGVRELVTHIENTVINTLSQFTIESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNVNM
DLSPFLRINPCGYAGMEMIQLHDLIKENDTVENKAEHVEDVHPLLIKELTTLLDYTDIEYITESSHHE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=154, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87081767, Length=220, Percent_Identity=62.7272727272727, Blast_Score=295, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6323268, Length=163, Percent_Identity=34.3558282208589, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=159, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25780; Mature: 25780

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLNKRLVVKNLGRQDYEPVWKAMHTFTDERDENSCDEVWLVEHNPVFTQGQAGKEEHV
CEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCE
LAAGDIPVVKSDRGGQVTYHGPGQLVAYVLINLRRKKIGVRELVTHIENTVINTLSQFTI
EEECCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNVNMDLSPFLRINPCGYAGMEMIQ
HHCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHHH
LHDLIKENDTVENKAEHVEDVHPLLIKELTTLLDYTDIEYITESSHHE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MILLNKRLVVKNLGRQDYEPVWKAMHTFTDERDENSCDEVWLVEHNPVFTQGQAGKEEHV
CEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCE
LAAGDIPVVKSDRGGQVTYHGPGQLVAYVLINLRRKKIGVRELVTHIENTVINTLSQFTI
EEECCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESAARPDAPGVYVDNKKICSLGLRIRKGCSFHGLALNVNMDLSPFLRINPCGYAGMEMIQ
HHCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHHH
LHDLIKENDTVENKAEHVEDVHPLLIKELTTLLDYTDIEYITESSHHE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA