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The map label for this gene is apbE [H]

Identifier: 209694522

GI number: 209694522

Start: 1052339

End: 1053376

Strand: Direct

Name: apbE [H]

Synonym: VSAL_I0955

Alternate gene names: 209694522

Gene position: 1052339-1053376 (Clockwise)

Preceding gene: 209694521

Following gene: 209694523

Centisome position: 31.65

GC content: 37.48

Gene sequence:

>1038_bases
GTGATTGTCAGTGTTACTCATTTAGTGAATAGAACCAAACTGATTTTGTTATCTTGTTTTGCTTTACTACTACTTACCGG
TTGTGGTGAACAACAACGTGAACAAGTGTATTTAAATGGTCCAACGATGGGGACAAGCTATAATATTAAATTTATTAATA
AAGATGGCTTACCAAAGCAAGATGTTATTCATGCAGAGATAGATAAACGACTAGAGTTAGTAAATGACCAAATGTCGACG
TACCGTAAAGACTCAGAACTGAGTCAATTTAATCAGAGTACAGCATTAACGCCATTTACGGTTTCTAAAGATACAGCCAC
AGTGATTAGTGAAGCGATTCGCTTAAATAAAGTCACTTTGGGTGCATTAGATATCACGGTTGGTCCTTTAGTTAATATAT
GGGGTTTTGGTCCAGAAGCTCGTCCTGAGGTTGTTCCTACTGAAGATGAATTGAATGCTCGTCGTGCTATTGTCGGTATT
CAGCATTTAAGTGCGACAGAAACAACCGTTACAAAAGATATCCCTAACCTTTATGTTGATTTATCAACAATAGCAAAAGG
CTGGGGAGTTGACGTTGTTGCTAATTATTTAGAAGGATTAGGCATTACTGACTATATGGTCGAAGTCGGTGGTGAAATGC
GAGTTAAAGGGAATAATAAAGAGAATGTTGCTTGGCGTATCGCGATAGAAAAGCCAACGGTTGATGAACGTTCTATTCAA
GAAATAATAGAACCAGGGCATCATGCGGTTGCAACGTCTGGTGATTACCGTATTTACTTTGAAAAAGAAGGTGTACGTTA
CTCTCATATTATTAATCCTATTACAGGGAACCCAATAGCAAATAAAGTTGTCTCAGTTACAGTACTTGATAAATCATCAA
TGACTGCTGATGGACTTGCTACAGGGTTAATGGTGTTAGGTGAAGAGCAAGGCATGGCCGTTGCGAATAAACATAACATT
CCTGTTTTCATGATTGTGAAAACTAAAAATGGCTTTAAAGAACTCTCTTCTGAAGCCTTTAAGCCTTTTTTGAAATAA

Upstream 100 bases:

>100_bases
TACATTTAACCAGACAAAGTACAGGGTTATAGGTTTTTTGGAATGTATATTCCTGTGTATTGTTTTGTTATTTAAATTTA
TGTTAATTAAGGAGTTGGCA

Downstream 100 bases:

>100_bases
GTGAGTAGATAATGAATACTTTTCTAATTACGTTTGGTGTTTTTCTTACTGTTATTGGTGCAATGGCTATTGGTTATATT
GTACAACGTAAAGTAGTGAA

Product: thiamine biosynthesis lipoprotein ApbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIVSVTHLVNRTKLILLSCFALLLLTGCGEQQREQVYLNGPTMGTSYNIKFINKDGLPKQDVIHAEIDKRLELVNDQMST
YRKDSELSQFNQSTALTPFTVSKDTATVISEAIRLNKVTLGALDITVGPLVNIWGFGPEARPEVVPTEDELNARRAIVGI
QHLSATETTVTKDIPNLYVDLSTIAKGWGVDVVANYLEGLGITDYMVEVGGEMRVKGNNKENVAWRIAIEKPTVDERSIQ
EIIEPGHHAVATSGDYRIYFEKEGVRYSHIINPITGNPIANKVVSVTVLDKSSMTADGLATGLMVLGEEQGMAVANKHNI
PVFMIVKTKNGFKELSSEAFKPFLK

Sequences:

>Translated_345_residues
MIVSVTHLVNRTKLILLSCFALLLLTGCGEQQREQVYLNGPTMGTSYNIKFINKDGLPKQDVIHAEIDKRLELVNDQMST
YRKDSELSQFNQSTALTPFTVSKDTATVISEAIRLNKVTLGALDITVGPLVNIWGFGPEARPEVVPTEDELNARRAIVGI
QHLSATETTVTKDIPNLYVDLSTIAKGWGVDVVANYLEGLGITDYMVEVGGEMRVKGNNKENVAWRIAIEKPTVDERSIQ
EIIEPGHHAVATSGDYRIYFEKEGVRYSHIINPITGNPIANKVVSVTVLDKSSMTADGLATGLMVLGEEQGMAVANKHNI
PVFMIVKTKNGFKELSSEAFKPFLK
>Mature_345_residues
MIVSVTHLVNRTKLILLSCFALLLLTGCGEQQREQVYLNGPTMGTSYNIKFINKDGLPKQDVIHAEIDKRLELVNDQMST
YRKDSELSQFNQSTALTPFTVSKDTATVISEAIRLNKVTLGALDITVGPLVNIWGFGPEARPEVVPTEDELNARRAIVGI
QHLSATETTVTKDIPNLYVDLSTIAKGWGVDVVANYLEGLGITDYMVEVGGEMRVKGNNKENVAWRIAIEKPTVDERSIQ
EIIEPGHHAVATSGDYRIYFEKEGVRYSHIINPITGNPIANKVVSVTVLDKSSMTADGLATGLMVLGEEQGMAVANKHNI
PVFMIVKTKNGFKELSSEAFKPFLK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=347, Percent_Identity=39.4812680115274, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37959; Mature: 37959

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSVTHLVNRTKLILLSCFALLLLTGCGEQQREQVYLNGPTMGTSYNIKFINKDGLPKQ
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCEEEEEEECCCCCCHH
DVIHAEIDKRLELVNDQMSTYRKDSELSQFNQSTALTPFTVSKDTATVISEAIRLNKVTL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCEEEE
GALDITVGPLVNIWGFGPEARPEVVPTEDELNARRAIVGIQHLSATETTVTKDIPNLYVD
EEEEEECCCHHHEECCCCCCCCCCCCCCHHHCHHHHEEEHHHCCCCCHHHHHCCCHHEEE
LSTIAKGWGVDVVANYLEGLGITDYMVEVGGEMRVKGNNKENVAWRIAIEKPTVDERSIQ
HHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCEEEEEEEECCCCCHHHHH
EIIEPGHHAVATSGDYRIYFEKEGVRYSHIINPITGNPIANKVVSVTVLDKSSMTADGLA
HHHCCCCCEEEECCCEEEEEECCCCEEEHEECCCCCCCHHCCEEEEEEECCCCCCHHHHH
TGLMVLGEEQGMAVANKHNIPVFMIVKTKNGFKELSSEAFKPFLK
HHHEEEECCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVSVTHLVNRTKLILLSCFALLLLTGCGEQQREQVYLNGPTMGTSYNIKFINKDGLPKQ
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCEEEEEEECCCCCCHH
DVIHAEIDKRLELVNDQMSTYRKDSELSQFNQSTALTPFTVSKDTATVISEAIRLNKVTL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCEEEE
GALDITVGPLVNIWGFGPEARPEVVPTEDELNARRAIVGIQHLSATETTVTKDIPNLYVD
EEEEEECCCHHHEECCCCCCCCCCCCCCHHHCHHHHEEEHHHCCCCCHHHHHCCCHHEEE
LSTIAKGWGVDVVANYLEGLGITDYMVEVGGEMRVKGNNKENVAWRIAIEKPTVDERSIQ
HHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCEEEEEEEECCCCCHHHHH
EIIEPGHHAVATSGDYRIYFEKEGVRYSHIINPITGNPIANKVVSVTVLDKSSMTADGLA
HHHCCCCCEEEECCCEEEEEECCCCEEEHEECCCCCCCHHCCEEEEEEECCCCCCHHHHH
TGLMVLGEEQGMAVANKHNIPVFMIVKTKNGFKELSSEAFKPFLK
HHHEEEECCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]