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The map label for this gene is glnS

Identifier: 209694411

GI number: 209694411

Start: 927768

End: 929426

Strand: Direct

Name: glnS

Synonym: VSAL_I0832

Alternate gene names: 209694411

Gene position: 927768-929426 (Clockwise)

Preceding gene: 209694410

Following gene: 209694413

Centisome position: 27.9

GC content: 38.58

Gene sequence:

>1659_bases
ATGAGTGAAACTGAAACTCGTCCTACCAACTTTATCCGTCAAATTATTGATGAAGACCTAAAGTCAGGTAAACATTCCAG
TGTCCATACTCGTTTCCCGCCAGAGCCAAACGGTTATCTGCATATCGGACACGCAAAATCTATCTGTTTGAATTTTGGTA
TTGCTCAAGATTACCAGGGACAGTGTAATCTACGTTTTGATGATACTAACCCTGAAAAAGAAGATATTGAATACGTTGAA
TCAATCAAAAATGACGTTAAATGGTTAGGTTTTGATTGGAGTGGTGATATTCATTATTCTTCGAATTACTTCGATAAATT
GTATGATTACGCGGTCGAATTGATTGAGAAAGGACTGGCTTATGTTGATGAGTTAACCTCTGATCAAATCCGTGAGTACC
GTGGTTCTCTTAAAGAGCCGGGTAAAAATAGCCCATACCGTGATCGTAGTGTCGAAGAGAACCTTGCGCTATTTGAACAA
ATGCGTGATGGTAAATTTAAAGAAGGCACTATTTGCTTACGTGCAAAAATCGACATGGCTTCTTCATTTATCGTTATGCG
CGATCCTGTTATTTATCGTATTCGTTTTGCTACTCATCACCAAACTGGCGATAAGTGGTGTATTTACCCAATGTACGATT
TTACTCATTGTATTTCGGATGCACTTGAAGGCATTACGCATTCTATCTGTACATTAGAATTCCAAGACAACCGTCGTTTA
TATGATTGGGTTTTGGAAAATATTACGATTGATTGCCAACCACATCAATATGAGTTTAGCCGTTTAAACTTAGAATACAC
GATCATGTCTAAGCGTAAGCTTAATGAACTGGTTACTGAGAAATTAGTAAACGGTTGGGATGATCCACGTATGCCTACGG
TTTCTGGTTTACGTCGTCGTGGTTTTACTGCGGGGTCTATTCGTGAATTCTGTAAGCGCATTGGTGTAACTAAGCAAGAG
AACATGATTGAATTTGGTTCATTAGAATCGTGTATTCGTGATGATCTAAATGAAAATGCACCTCGTGCAATGGCTGTACT
TGAACCTGTAAAAGTAGTGATTGAAAACTACGAAGAAGGTAAAGTCGAAACGCTAAACGTTGCAAATCATCCAAATAAAC
CAGAAATGGGTACTCGTGAAGTTCCATTTACTCGTGAAATTTATATTGAGCAAGATGATTTCCGTGAAGAAGCAAACAAG
AAATACAAACGTTTAGTACTTGGTAAAGAAGTGCGTTTACGCGGTGCTTATGTTATTCAAGCAAATCGAATTGAAAAAGA
CGAAGCGGGTAACATCACGACTATTTTCTGTAGCTATGATACAGAAACGCTAGGTAAAAACCCAGCAGATGGTCGTAAAG
TGAAGGGCGTTATTCATTGGGTATCTGCAGACAAAGCGTTCCCTGCAGAAATTCGTCTATATGATCGTTTATTCACAGTG
CCAAACTCTGCGGCTTTAGAGGATTTTTCAGAAAGTATTAATCCTGAATCATTAATTACTAAACAGGGTTTTGTTGAACC
AAGTTTAGTAACTGCAGAAGCGGAAGCTGGCTATCAATTTGAACGTACAGGCTATTTCTGTATTGATAACAAAGACTCTA
CTCCAGAGGCTCTTGTATTCAACCGTACAGTAGGTTTACGTGATACTTGGGAAGGTTAA

Upstream 100 bases:

>100_bases
AACTATTTGATTCTATATATTGAGTATTGGCCCATAATTGTGGATCATAGGGAGATATGAACAATTTGTTCTTTCTCCTA
ATACCAATGAGGTGCTTTAG

Downstream 100 bases:

>100_bases
TCCTAATCAAGTAATTTATAGATAATAAAAAACCAGCAGAGATGCTGGTTTTTTTATGCACGTAAACTAAATATTAGCCA
TTATGAGCAGTGGTATTTTC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSETETRPTNFIRQIIDEDLKSGKHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDIEYVE
SIKNDVKWLGFDWSGDIHYSSNYFDKLYDYAVELIEKGLAYVDELTSDQIREYRGSLKEPGKNSPYRDRSVEENLALFEQ
MRDGKFKEGTICLRAKIDMASSFIVMRDPVIYRIRFATHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLENITIDCQPHQYEFSRLNLEYTIMSKRKLNELVTEKLVNGWDDPRMPTVSGLRRRGFTAGSIREFCKRIGVTKQE
NMIEFGSLESCIRDDLNENAPRAMAVLEPVKVVIENYEEGKVETLNVANHPNKPEMGTREVPFTREIYIEQDDFREEANK
KYKRLVLGKEVRLRGAYVIQANRIEKDEAGNITTIFCSYDTETLGKNPADGRKVKGVIHWVSADKAFPAEIRLYDRLFTV
PNSAALEDFSESINPESLITKQGFVEPSLVTAEAEAGYQFERTGYFCIDNKDSTPEALVFNRTVGLRDTWEG

Sequences:

>Translated_552_residues
MSETETRPTNFIRQIIDEDLKSGKHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDIEYVE
SIKNDVKWLGFDWSGDIHYSSNYFDKLYDYAVELIEKGLAYVDELTSDQIREYRGSLKEPGKNSPYRDRSVEENLALFEQ
MRDGKFKEGTICLRAKIDMASSFIVMRDPVIYRIRFATHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLENITIDCQPHQYEFSRLNLEYTIMSKRKLNELVTEKLVNGWDDPRMPTVSGLRRRGFTAGSIREFCKRIGVTKQE
NMIEFGSLESCIRDDLNENAPRAMAVLEPVKVVIENYEEGKVETLNVANHPNKPEMGTREVPFTREIYIEQDDFREEANK
KYKRLVLGKEVRLRGAYVIQANRIEKDEAGNITTIFCSYDTETLGKNPADGRKVKGVIHWVSADKAFPAEIRLYDRLFTV
PNSAALEDFSESINPESLITKQGFVEPSLVTAEAEAGYQFERTGYFCIDNKDSTPEALVFNRTVGLRDTWEG
>Mature_551_residues
SETETRPTNFIRQIIDEDLKSGKHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDIEYVES
IKNDVKWLGFDWSGDIHYSSNYFDKLYDYAVELIEKGLAYVDELTSDQIREYRGSLKEPGKNSPYRDRSVEENLALFEQM
RDGKFKEGTICLRAKIDMASSFIVMRDPVIYRIRFATHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRLY
DWVLENITIDCQPHQYEFSRLNLEYTIMSKRKLNELVTEKLVNGWDDPRMPTVSGLRRRGFTAGSIREFCKRIGVTKQEN
MIEFGSLESCIRDDLNENAPRAMAVLEPVKVVIENYEEGKVETLNVANHPNKPEMGTREVPFTREIYIEQDDFREEANKK
YKRLVLGKEVRLRGAYVIQANRIEKDEAGNITTIFCSYDTETLGKNPADGRKVKGVIHWVSADKAFPAEIRLYDRLFTVP
NSAALEDFSESINPESLITKQGFVEPSLVTAEAEAGYQFERTGYFCIDNKDSTPEALVFNRTVGLRDTWEG

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI4826960, Length=527, Percent_Identity=45.73055028463, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI62241042, Length=517, Percent_Identity=34.0425531914894, Blast_Score=291, Evalue=7e-79,
Organism=Escherichia coli, GI1786895, Length=550, Percent_Identity=79.0909090909091, Blast_Score=932, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=122, Percent_Identity=28.6885245901639, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=530, Percent_Identity=43.2075471698113, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=33.9731285988484, Blast_Score=267, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=42.4511545293073, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321192, Length=541, Percent_Identity=36.4140480591497, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17864488, Length=528, Percent_Identity=44.1287878787879, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24649466, Length=521, Percent_Identity=35.3166986564299, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYQ_ALISL (B6EHL7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262339.1
- GeneID:   6988880
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0832
- HOGENOM:   HBG334108
- OMA:   RMPTIAG
- ProtClustDB:   PRK05347
- GO:   GO:0005737
- HAMAP:   MF_00126
- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119:SF3
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00440

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.18

Molecular weight: Translated: 63726; Mature: 63595

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETETRPTNFIRQIIDEDLKSGKHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKEDIEYVESIKNDVKWLGFDWSGDIHYSSNYFDKLYDYAVELIEKGLA
CEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTSDQIREYRGSLKEPGKNSPYRDRSVEENLALFEQMRDGKFKEGTICLRAKIDMA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
SSFIVMRDPVIYRIRFATHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
YDWVLENITIDCQPHQYEFSRLNLEYTIMSKRKLNELVTEKLVNGWDDPRMPTVSGLRRR
HHHHHHCCEEECCCCCEEHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTAGSIREFCKRIGVTKQENMIEFGSLESCIRDDLNENAPRAMAVLEPVKVVIENYEEG
CCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
KVETLNVANHPNKPEMGTREVPFTREIYIEQDDFREEANKKYKRLVLGKEVRLRGAYVIQ
CEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
ANRIEKDEAGNITTIFCSYDTETLGKNPADGRKVKGVIHWVSADKAFPAEIRLYDRLFTV
ECCCCCCCCCCEEEEEEECCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHEEEHHHHCC
PNSAALEDFSESINPESLITKQGFVEPSLVTAEAEAGYQFERTGYFCIDNKDSTPEALVF
CCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCEEEECCCEEEECCCCCCCCEEEE
NRTVGLRDTWEG
ECCCCCCCCCCC
>Mature Secondary Structure 
SETETRPTNFIRQIIDEDLKSGKHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKEDIEYVESIKNDVKWLGFDWSGDIHYSSNYFDKLYDYAVELIEKGLA
CEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTSDQIREYRGSLKEPGKNSPYRDRSVEENLALFEQMRDGKFKEGTICLRAKIDMA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
SSFIVMRDPVIYRIRFATHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
YDWVLENITIDCQPHQYEFSRLNLEYTIMSKRKLNELVTEKLVNGWDDPRMPTVSGLRRR
HHHHHHCCEEECCCCCEEHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTAGSIREFCKRIGVTKQENMIEFGSLESCIRDDLNENAPRAMAVLEPVKVVIENYEEG
CCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
KVETLNVANHPNKPEMGTREVPFTREIYIEQDDFREEANKKYKRLVLGKEVRLRGAYVIQ
CEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
ANRIEKDEAGNITTIFCSYDTETLGKNPADGRKVKGVIHWVSADKAFPAEIRLYDRLFTV
ECCCCCCCCCCEEEEEEECCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHEEEHHHHCC
PNSAALEDFSESINPESLITKQGFVEPSLVTAEAEAGYQFERTGYFCIDNKDSTPEALVF
CCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCEEEECCCEEEECCCCCCCCEEEE
NRTVGLRDTWEG
ECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA