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The map label for this gene is nagE [H]

Identifier: 209694410

GI number: 209694410

Start: 926109

End: 927599

Strand: Direct

Name: nagE [H]

Synonym: VSAL_I0831

Alternate gene names: 209694410

Gene position: 926109-927599 (Clockwise)

Preceding gene: 209694405

Following gene: 209694411

Centisome position: 27.85

GC content: 42.05

Gene sequence:

>1491_bases
GTGAATATTTTAGGAAAGTTTCAAAAAGTAGGTAAAGCACTGATGGTACCAGTTGCTACATTACCTGCAGCGGCCATTTT
AATGGGTATTGGTTACTGGATTGATCCGGTGGCTTGGGGCGGCAATAGTGCATTAGCAGCGTTTTTAATCAAAGCTGGTG
CGTCTATTATTGATAACATGTCTGTACTATTCGCAATTGGTGTTGCTTATGGTATGTCTAAAGATAAAGATGGTGCAGCG
GCACTTTCTGGTTTTGTTGGTTTCCTTGTTGTAACGAATCTTCTTTCACCAGGTGCGGTAGCTCAAATTCAAGGCATTGA
TCCTGCTGCTGTTCCTGCTGCTTTTAGTAAGATCAACAACCAATTTGTTGGTATTTTAGTTGGTATCATATCTGCTGAAA
TTTACAACCGTTTCTCTGCTGTTGAATTGCATAAAACACTTGCATTCTTCTCAGGTAAACGTCTTGTACCTATTCTAACG
TCATTTGCAGGTATTTTAATTGCGTTCGTGCTTATGTACGTATGGCCAGCAATCTACGATGGTCTTGTTCACTTCGGCGA
ATCAATTCAAGGTATGGGTGCTACGGGTGCAGGTATCTATGCATTCTTCAACCGTCTATTGATTCCTGTAGGTCTTCACC
ATGCACTTAACTCAGTGTTCTGGTTCGACGTTGCTGGTATTAACGACATCCCTAACTTCCTAGGTGGCGCTAAGTCTATC
GCTGAAGGTACTGCAACTGTCGGTGTTACCGGTATGTACCAAGCTGGTTTCTTCCCAATCATGATGTTTGGTCTACCAGG
CGCAGCACTAGCTATCTACCACACTGCAAAAGTTGAGAACAAAGAAAAAGTAGCATCGATTATGATCGCTGCAGGTTTCG
CATCGTTCTTTACGGGTGTAACTGAACCTCTAGAATTCTCATTCATGTTCCTTGCTCCTGCATTGTATGTAGTTCACGCA
GCATTGACGGGTATCTCTGTATTCATCGCAGCATCTATGCATTGGATTGCAGGCTTCGGTTTCTCTGCCGGCCTAGTTGA
TATGGTTCTTTCTAGTCGTAACCCACTAGCGGTTAATTGGTATATGCTAATTATCCAAGGTATCGTTTTCTTCGCTCTAT
ACTACTTTATCTTCCGCGCTGTAATCCTTAAGTTTAACCTTAAGACTCCAGGACGTGAAGACGATGATGAAGAAGAAAGC
TCAATCCGTGGTTCTAAAGAAACTGGTGAACTAGCTAAGCAATACCTTAAAGCGCTTGGTGGTCACGACAATATAGAAAA
CATTGATGCTTGTATTACTCGTTTACGCTTAACTCTTAGAGATACTAGCGTAATCAGCGAGAAGCAACTTAAAGGTCTTG
GTGCAATGGGTGTTGTTAAACTTGGTTCAAACAATGTTCAAGTTATCCTAGGTCCTCTTACTGAAATCGTTGCTGGTGAA
ATGAAAAAAATCCCAGCGTCTGAAGACTTAACAGCAGTTGCACTACCATAA

Upstream 100 bases:

>100_bases
TTACACTGACCCTGTTAATAACGAGTAGCGAAATAAGTTTATAATAAGCTAACGTAAGCTACTCAAAAAAATCTAAAATC
CTTATAGGGGGAATCTAAAG

Downstream 100 bases:

>100_bases
TTATTGAGTAAATTAACTCGATTTAATAAGCCGCTCTAATTAGAGCGGCTTTTTTATGGAAATAAAGCAACTATTTGATT
CTATATATTGAGTATTGGCC

Product: PTS permease for N-acetylglucosamine and glucose

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNILGKFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGASIIDNMSVLFAIGVAYGMSKDKDGAA
ALSGFVGFLVVTNLLSPGAVAQIQGIDPAAVPAAFSKINNQFVGILVGIISAEIYNRFSAVELHKTLAFFSGKRLVPILT
SFAGILIAFVLMYVWPAIYDGLVHFGESIQGMGATGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLAVNWYMLIIQGIVFFALYYFIFRAVILKFNLKTPGREDDDEEES
SIRGSKETGELAKQYLKALGGHDNIENIDACITRLRLTLRDTSVISEKQLKGLGAMGVVKLGSNNVQVILGPLTEIVAGE
MKKIPASEDLTAVALP

Sequences:

>Translated_496_residues
MNILGKFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGASIIDNMSVLFAIGVAYGMSKDKDGAA
ALSGFVGFLVVTNLLSPGAVAQIQGIDPAAVPAAFSKINNQFVGILVGIISAEIYNRFSAVELHKTLAFFSGKRLVPILT
SFAGILIAFVLMYVWPAIYDGLVHFGESIQGMGATGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLAVNWYMLIIQGIVFFALYYFIFRAVILKFNLKTPGREDDDEEES
SIRGSKETGELAKQYLKALGGHDNIENIDACITRLRLTLRDTSVISEKQLKGLGAMGVVKLGSNNVQVILGPLTEIVAGE
MKKIPASEDLTAVALP
>Mature_496_residues
MNILGKFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGASIIDNMSVLFAIGVAYGMSKDKDGAA
ALSGFVGFLVVTNLLSPGAVAQIQGIDPAAVPAAFSKINNQFVGILVGIISAEIYNRFSAVELHKTLAFFSGKRLVPILT
SFAGILIAFVLMYVWPAIYDGLVHFGESIQGMGATGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGVTEPLEFSFMFLAPALYVVHA
ALTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLAVNWYMLIIQGIVFFALYYFIFRAVILKFNLKTPGREDDDEEES
SIRGSKETGELAKQYLKALGGHDNIENIDACITRLRLTLRDTSVISEKQLKGLGAMGVVKLGSNNVQVILGPLTEIVAGE
MKKIPASEDLTAVALP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=497, Percent_Identity=44.6680080482897, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1787343, Length=497, Percent_Identity=38.2293762575453, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1787908, Length=514, Percent_Identity=35.0194552529183, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 52715; Mature: 52715

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGKFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGASIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SVLFAIGVAYGMSKDKDGAAALSGFVGFLVVTNLLSPGAVAQIQGIDPAAVPAAFSKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
QFVGILVGIISAEIYNRFSAVELHKTLAFFSGKRLVPILTSFAGILIAFVLMYVWPAIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVHFGESIQGMGATGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
HHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCHHHH
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGV
HCCCHHCCCCHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPALYVVHAALTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLAVNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHH
YMLIIQGIVFFALYYFIFRAVILKFNLKTPGREDDDEEESSIRGSKETGELAKQYLKALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHC
GHDNIENIDACITRLRLTLRDTSVISEKQLKGLGAMGVVKLGSNNVQVILGPLTEIVAGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHH
MKKIPASEDLTAVALP
HHCCCCCCCCEEEECC
>Mature Secondary Structure
MNILGKFQKVGKALMVPVATLPAAAILMGIGYWIDPVAWGGNSALAAFLIKAGASIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SVLFAIGVAYGMSKDKDGAAALSGFVGFLVVTNLLSPGAVAQIQGIDPAAVPAAFSKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHH
QFVGILVGIISAEIYNRFSAVELHKTLAFFSGKRLVPILTSFAGILIAFVLMYVWPAIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVHFGESIQGMGATGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSI
HHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCHHHH
AEGTATVGVTGMYQAGFFPIMMFGLPGAALAIYHTAKVENKEKVASIMIAAGFASFFTGV
HCCCHHCCCCHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPALYVVHAALTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLAVNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHH
YMLIIQGIVFFALYYFIFRAVILKFNLKTPGREDDDEEESSIRGSKETGELAKQYLKALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHC
GHDNIENIDACITRLRLTLRDTSVISEKQLKGLGAMGVVKLGSNNVQVILGPLTEIVAGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHH
MKKIPASEDLTAVALP
HHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]